BBa_K875003
1
BBa_K875003
CymR
2012-09-22T11:00:00Z
2015-05-08T01:13:38Z
1. The cumate gene-switch: a system for regulated expression in mammalian cells
Alaka Mullick, Yan Xu, Ren?? Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, F??lix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 )
2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains
Young J. Choi, Lyne Morel, Teffanie Le Fran??ois Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. M??guez (Applied and Environmental Microbiology, Aug. 2010, p. 5058???5066 Vol.76 doi:10.1128/AEM.00413-10)
This composite produces constitutively the CymR regulator protein, that binds the Cumate Operator repressing the transcription from the promoter.
false
false
_1136_
0
12660
9
It's complicated
true
The CymR has a SV5 tag at the C-terminus in order to be detected by an anti-SV5 Ab.
false
Federico Colombo, Elisa Clagnan
annotation2194312
1
BBa_B0034
range2194312
1
44
55
annotation2194311
1
CymR
range2194311
1
62
679
annotation2194313
1
SV5 Tag
range2194313
1
680
724
annotation2194310
1
BBa_J23100
range2194310
1
1
35
annotation2194314
1
BBa_B0015
range2194314
1
739
867
BBa_K875003_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggtgatcatgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgcagcaggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgcggcaaaccaatcccaaacccactgctgggcctggatagtacttgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z