BBa_K875003 1 BBa_K875003 CymR 2012-09-22T11:00:00Z 2015-05-08T01:13:38Z 1. The cumate gene-switch: a system for regulated expression in mammalian cells Alaka Mullick, Yan Xu, Ren?? Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, F??lix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 ) 2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains Young J. Choi, Lyne Morel, Teffanie Le Fran??ois Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. M??guez (Applied and Environmental Microbiology, Aug. 2010, p. 5058???5066 Vol.76 doi:10.1128/AEM.00413-10) This composite produces constitutively the CymR regulator protein, that binds the Cumate Operator repressing the transcription from the promoter. false false _1136_ 0 12660 9 It's complicated true The CymR has a SV5 tag at the C-terminus in order to be detected by an anti-SV5 Ab. false Federico Colombo, Elisa Clagnan annotation2194312 1 BBa_B0034 range2194312 1 44 55 annotation2194311 1 CymR range2194311 1 62 679 annotation2194313 1 SV5 Tag range2194313 1 680 724 annotation2194310 1 BBa_J23100 range2194310 1 1 35 annotation2194314 1 BBa_B0015 range2194314 1 739 867 BBa_K875003_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggtgatcatgagtccaaagagaagaacacaggcagagcgcgcaatggagacccagggcaagttgattgcagcggccctgggggttttacgggaaaaaggttacgcgggattccggatcgcagatgtgcccggtgcagcaggtgtctcgagaggagcgcagagccatcatttcccgacaaagcttgagcttctgcttgccacttttgaatggctttacgaacagatcaccgaacgcagtcgggctcgattagcgaaattgaagccagaggatgacgtcatccagcaaatgctggacgacgccgccgaatttttcctcgacgatgacttctctatcagccttgatttgattgtggctgccgaccgggatccagcgttacgcgagggtattcagcgcacggtagagaggaatcggtttgtcgtcgaggatatgtggcttggtgttctggtgagccgtggtctttcgcgtgatgatgcagaagatatcctttggttgatattcaattcggtgcgtgggcttgctgttcgtagcctatggcagaaggacaaagaacgctttgagcgtgtcaggaactcgacactcgaaattgcgcgagagcggtacgcgaaattcaagcgcggcaaaccaatcccaaacccactgctgggcctggatagtacttgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z