BBa_K875006
1
BBa_K875006
T5LacO-PelB-scFv (scFv 54.6 antinorovirus)-6HIS-TT
2012-09-22T11:00:00Z
2015-05-08T01:13:38Z
Gene synthesis.
Reference:
???Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands??? K. Ettayebi and M. E. Hardy. Published: 31 January 2008 in Virology Journal 2008, 5:21?
This construct is designed for the expression of secreted, already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6).
The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. PelB is a leader sequence often used to produce secreted proteins.
The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-scFv 54.6 antinorovirus, HIstidine tag (6HIS), Terminator (B0015).
false
false
_1136_
0
12664
9
It's complicated
false
This coding sequence was optimized for expression into the prokaryotic cells. It has a Histidine tag.
false
Marija Drikic, Giorgio Gargari
annotation2194479
1
protein
range2194479
1
101
958
annotation2194477
1
tag
range2194477
1
965
982
annotation2194476
1
rbs
range2194476
1
89
100
annotation2194475
1
promoter
range2194475
1
1
80
annotation2194478
1
stem_loop
range2194478
1
991
1121
BBa_K875006_sequence
1
aatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggatgatattctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattgggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctgaccgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z