BBa_K875006 1 BBa_K875006 T5LacO-PelB-scFv (scFv 54.6 antinorovirus)-6HIS-TT 2012-09-22T11:00:00Z 2015-05-08T01:13:38Z Gene synthesis. Reference: ???Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands??? K. Ettayebi and M. E. Hardy. Published: 31 January 2008 in Virology Journal 2008, 5:21? This construct is designed for the expression of secreted, already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6). The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. PelB is a leader sequence often used to produce secreted proteins. The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-scFv 54.6 antinorovirus, HIstidine tag (6HIS), Terminator (B0015). false false _1136_ 0 12664 9 It's complicated false This coding sequence was optimized for expression into the prokaryotic cells. It has a Histidine tag. false Marija Drikic, Giorgio Gargari annotation2194476 1 rbs range2194476 1 89 100 annotation2194475 1 promoter range2194475 1 1 80 annotation2194479 1 protein range2194479 1 101 958 annotation2194477 1 tag range2194477 1 965 982 annotation2194478 1 stem_loop range2194478 1 991 1121 BBa_K875006_sequence 1 aatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggatgatattctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattgggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctgaccgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z