BBa_K875007 1 BBa_K875007 T5 LacO-PelB-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT 2012-09-22T11:00:00Z 2015-05-08T01:13:38Z Gene synthesis. Reference: ???Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands??? K. Ettayebi and M. E. Hardy. Published: 31 January 2008 in Virology Journal 2008, 5:21? This construct is designed for the expression of secreted, already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6). The antibody is expressed in a small immuno protein (SIP) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker and constant domain (CH3) of heavy chain of human immunoglobulin A (IgA). It has already been reported that the scFv 54.6 (which makes part of SIP) binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. Alpha isotype CH3 domain homodimerizes confering bivalent binding properties to the antibody. PelB is a leader sequence often used to produce secreted proteins. The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-SIP 54.6 antinorovirus, Hystidine tag (6HIS), Terminator (B0015). false false _1136_ 0 12664 9 It's complicated false This sequence was optimized for the expression in prokaryotic cells. It has a Histidine tag. false Marija Drikic, Giorgio Gargari annotation2194338 1 promoter range2194338 1 1 80 annotation2194403 1 protein range2194403 1 101 1366 annotation2194339 1 rbs range2194339 1 89 100 annotation2194362 1 tag range2194362 1 1373 1390 annotation2194348 1 stem_loop range2194348 1 1402 1529 BBa_K875007_sequence 1 aatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggatgatattctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtgagctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcaccaacggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcagcggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagctggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggcaaaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaaactgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattgggtgcgccagaccccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccagataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaagcgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctgaccgtgagcagctccggaggctctgggggcaacacctttcgcccggaagtgcatctgctgccgccgccgagcgaagaactggcgctgaacgaactggtgaccctgacctgcctggcgcgcggctttagcccgaaagatgtgctggtgcgctggctacagggcagccaggaactgccgcgcgaaaaatatctgacctgggcgagccgccaggaaccgagccagggcaccaccacctttgcggtgaccagcattctgcgcgtggcggcggaagattggaaaaaaggcgatacctttagctgcatggtgggccatgaagcgctgccgctggcgtttacccagaaaaccattgatcgcctggcgggcaaaccgacccatgtgaacgtgagcgtggtgatggcggaagtggatggcacctgctataccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z