LOCUS       BBa_K875008              722 bp    DNA     linear       22-Nov-2024
DEFINITION  IPTG inducible Tse2 toxin
ACCESSION   BBa_K875008
VERSION     BBa_K875008.1  
FEATURES             Location/Qualifiers
     promoter        1..80
                     /label=BBa_K875002
     misc_feature    95..98
                     /label=conserved
     RBS             89..102
                     /label=BBa_B0031
     misc_feature    109..585
                     /label=tse2
     CDS             109..585
                     /label=BBa_K314200
     stem_loop       605..648
                     /label=stem_loop
     misc_feature    594..673
                     /label=BBa_B0010
     terminator      594..673
                     /label=BBa_B0010
     stem_loop       689..708
                     /label=T7 TE
     misc_feature    715..715
                     /label=stop
     polyA_site      709..722
                     /label=polya
     misc_feature    682..722
                     /label=BBa_B0012
     terminator      682..722
                     /label=BBa_B0012
     terminator      594..722
                     /label=BBa_B0015
ORIGIN
        1 aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat tcaattgtga
       61 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat gtcctacgac
      121 tacgagaaaa ccagcctcac cctctaccgg gcggtattca aggccaacta cgacggcgac
      181 gtcggtcgct acctgcatcc cgacaaggaa ctcgccgagg ctgcggaagt cgccccgctg
      241 ctgcatccga ccttcgacag ccccaacacc cctggcgtcc ccgcccgcgc gccggacatc
      301 gtcgccggcc gcgacggcct ctacgccccg gacaccggcg gcacctcggt gttcgaccgc
      361 gccggcgtgc tgcgccgcgc cgacggcgac ttcgtgatac ccgacggcac cgacatcccg
      421 ccggacctta aggtgaagca ggacagctac aacaagcgcc tgcaagccac ccactacacc
      481 atcatgccgg ccaagccgat gtaccgggag gtcctcatgg gccaactgga caacttcgtg
      541 cgcaacgcca tccgccgcca atgggaaaaa gcccgcgggc tctagtacta gagccaggca
      601 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      661 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      721 ta
//