LOCUS BBa_K876001 3827 bp DNA linear 08-Dec-2025 DEFINITION Toggle Mechanism ACCESSION BBa_K876001 VERSION BBa_K876001.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 1150..1188 /label=LVA CDS 61..1188 /label=lacI-LVA misc_feature 61..1188 /label=BBa_C0012 misc_feature 1189..1213 /label=Help:Barcodes CDS 61..1188 /label=BBa_C0012 RBS 1222..1236 /label=RBS-1\Strong RBS 1229..1233 /label=RBS misc_feature 1222..1236 /label=BBa_B0030 RBS 1222..1236 /label=BBa_B0030 CDS 1243..1962 /label=GFP protein CDS 1243..1962 /label=BBa_E0040 misc_feature 1971..2009 /label=B1006 stem_loop 1980..2001 /label=modified thr terminator polyA_site 2002..2009 /label=PolyA polyA_site 1971..1979 /label=PolyA terminator 1971..2009 /label=BBa_B1006 misc_feature 1222..2009 /label=BBa_K081012 stem_loop 2029..2072 /label=stem_loop misc_feature 2018..2097 /label=BBa_B0010 terminator 2018..2097 /label=BBa_B0010 stem_loop 2113..2132 /label=T7 TE misc_feature 2139..2139 /label=stop polyA_site 2133..2146 /label=polya misc_feature 2106..2146 /label=BBa_B0012 terminator 2106..2146 /label=BBa_B0012 terminator 2018..2146 /label=BBa_B0015 CDS 2155..2242 /label=end of LacI coding region (inactive) misc_feature 2155..2354 /label=BBa_R0010 misc_feature 2243..2280 /label=CAP binding site promoter 2291..2296 /label=-35 promoter 2315..2320 /label=-10 misc_feature 2320..2354 /label=LacI binding site misc_feature 2327..2327 /label=start promoter 2155..2354 /label=BBa_R0010 CDS 2364..2980 /label=tetR misc_feature 2981..3014 /label=SsrA misc_feature 3021..3045 /label=Help:Barcodes CDS 2361..3045 /label=BBa_C0040 RBS 3054..3068 /label=RBS-1\Strong RBS 3061..3065 /label=RBS misc_feature 3054..3068 /label=BBa_B0030 RBS 3054..3068 /label=BBa_B0030 CDS 3075..3749 /label=mrfp1 misc_feature 3756..3780 /label=Help:Barcodes CDS 3075..3780 /label=BBa_E1010 misc_feature 3789..3827 /label=B1006 stem_loop 3798..3819 /label=modified thr terminator polyA_site 3820..3827 /label=PolyA polyA_site 3789..3797 /label=PolyA terminator 3789..3827 /label=BBa_B1006 misc_feature 3054..3827 /label=BBa_K081014 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 121 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 181 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 241 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 301 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 361 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 421 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 481 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 541 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 601 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 661 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 721 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 781 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 841 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 901 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 961 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 1021 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 1081 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 1141 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 1201 tagtgtagat ctctactaga gattaaagag gagaaatact agatgcgtaa aggagaagaa 1261 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 1321 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 1381 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 1441 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 1501 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 1561 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 1621 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 1681 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 1741 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 1801 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 1861 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct 1921 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag aaaaaaaaac 1981 cccgcccctg acagggcggg gttttttttt actagagcca ggcatcaaat aaaacgaaag 2041 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2101 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagcaatac 2161 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 2221 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 2281 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 2341 aacaatttca cacatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 2401 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 2461 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 2521 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 2581 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 2641 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 2701 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 2761 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 2821 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 2881 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 2941 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 3001 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagattaaag 3061 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag 3121 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc 3181 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt 3241 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc 3301 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg 3361 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg 3421 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta 3481 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg 3541 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg 3601 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg 3661 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac 3721 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc 3781 tactagagaa aaaaaaaccc cgcccctgac agggcggggt ttttttt //