##gff-version 3
##sequence-region BBa_K876001 1 3827
BBa_K876001	.	CDS	2361	3045	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K876001	.	engineered_tag	3021	3045	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K876001	.	tag	2981	3014	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K876001	.	CDS	2364	2980	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K876001	.	promoter	2155	2354	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K876001	.	non_covalent_binding_site	2320	2354	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K876001	.	non_covalent_binding_site	2243	2280	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K876001	.	engineered_region	2155	2354	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K876001	.	promoter	2291	2296	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K876001	.	CDS	2155	2242	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K876001	.	start_codon	2327	2327	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K876001	.	promoter	2315	2320	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K876001	.	terminator	2018	2146	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876001	.	terminator	2018	2097	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876001	.	stem_loop	2029	2072	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876001	.	engineered_region	2018	2097	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876001	.	terminator	2106	2146	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876001	.	engineered_region	2106	2146	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876001	.	stop_codon	2139	2139	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876001	.	stem_loop	2113	2132	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876001	.	polyA_site	2133	2146	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876001	.	engineered_region	1222	2009	.	+	0	ID=BBa_K081012;Name=BBa_K081012
BBa_K876001	.	CDS	1243	1962	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K081012
BBa_K876001	.	CDS	1243	1962	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K876001	.	ribosome_entry_site	1222	1236	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081012
BBa_K876001	.	engineered_region	1222	1236	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K876001	.	ribosome_entry_site	1229	1233	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K876001	.	ribosome_entry_site	1222	1236	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K876001	.	terminator	1971	2009	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081012
BBa_K876001	.	polyA_site	2002	2009	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K876001	.	polyA_site	1971	1979	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K876001	.	engineered_region	1971	2009	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K876001	.	stem_loop	1980	2001	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K876001	.	engineered_region	3054	3827	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K876001	.	ribosome_entry_site	3054	3068	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K876001	.	engineered_region	3054	3068	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K876001	.	ribosome_entry_site	3061	3065	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K876001	.	ribosome_entry_site	3054	3068	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K876001	.	terminator	3789	3827	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K876001	.	polyA_site	3820	3827	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K876001	.	polyA_site	3789	3797	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K876001	.	engineered_region	3789	3827	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K876001	.	stem_loop	3798	3819	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K876001	.	CDS	3075	3780	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K876001	.	CDS	3075	3749	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K876001	.	engineered_tag	3756	3780	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K876001	.	CDS	61	1188	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K876001	.	engineered_tag	1189	1213	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K876001	.	CDS	61	1188	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K876001	.	tag	1150	1188	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K876001	.	engineered_region	61	1188	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K876001	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K876001	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K876001	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K876001	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K876001	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K876001	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K876001
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggtgaatgtgaaaccag
taacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgt
ttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactg
gcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga
ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagc
ggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgct
gtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttct
cccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggccc
attaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcg
gaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactg
cgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgga
tatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatttt
cgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgc
ccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt
aatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaa
taactctgatagtgctagtgtagatctctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttt
tcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtga
aggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacactt
gtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtg
ccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaa
gtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacac
aaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttca
aaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccc
tgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatg
gtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaaaa
aaaaccccgcccctgacagggcggggtttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacga
caggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggct
ttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgtccagat
tagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaact
cgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcc
attgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacg
ctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaaca
gtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagc
gctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta
ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttatt
cggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactac
gctttagtagcttaataacactgatagtgctagtgtagatcactactagagattaaagaggagaaatactagatggctt
cctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaat
cgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccg
ttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggact
acctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttac
ccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccg
gttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaa
tcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggt
tcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacag
tacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaa
aaaaaccccgcccctgacagggcggggtttttttt