LOCUS       BBa_K876005             2217 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-RBS-GFP-T-pTet-LacI
ACCESSION   BBa_K876005
VERSION     BBa_K876005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..949
                     /label=GFP protein
     CDS             230..949
                     /label=BBa_E0040
     misc_feature    958..996
                     /label=B1006
     stem_loop       967..988
                     /label=modified thr terminator
     polyA_site      989..996
                     /label=PolyA
     polyA_site      958..966
                     /label=PolyA
     terminator      958..996
                     /label=BBa_B1006
     misc_feature    209..996
                     /label=BBa_K081012
     misc_feature    1005..1023
                     /label=TetR 1
     misc_feature    1005..1058
                     /label=BBa_R0040
     promoter        1024..1029
                     /label=-35
     misc_feature    1030..1048
                     /label=TetR 2
     promoter        1047..1052
                     /label=-10
     promoter        1005..1058
                     /label=BBa_R0040
     misc_feature    2154..2192
                     /label=LVA
     CDS             1065..2192
                     /label=lacI-LVA
     misc_feature    1065..2192
                     /label=BBa_C0012
     misc_feature    2193..2217
                     /label=Help:Barcodes
     CDS             1065..2192
                     /label=BBa_C0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcgtaaagg
      241 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      301 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      361 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      421 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      481 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      541 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      601 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      661 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      721 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      781 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      841 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      901 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagaaa
      961 aaaaaacccc gcccctgaca gggcggggtt tttttttact agagtcccta tcagtgatag
     1021 agattgacat ccctatcagt gatagagata ctgagcacta ctagatggtg aatgtgaaac
     1081 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
     1141 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
     1201 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
     1261 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
     1321 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
     1381 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
     1441 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
     1501 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
     1561 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
     1621 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
     1681 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
     1741 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
     1801 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
     1861 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
     1921 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     1981 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     2041 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2101 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa
     2161 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctc
//