LOCUS BBa_K876008 3409 bp DNA linear 22-Nov-2024 DEFINITION pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI ACCESSION BBa_K876008 VERSION BBa_K876008.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 207..208 /label=prefix misc_feature 380..380 /label=T misc_feature 698..698 /label=A CDS 207..956 /label=luxR misc_feature 207..962 /label=BBa_C0062 misc_feature 963..987 /label=Help:Barcodes CDS 207..962 /label=BBa_C0062 stem_loop 1007..1050 /label=stem_loop misc_feature 996..1075 /label=BBa_B0010 terminator 996..1075 /label=BBa_B0010 stem_loop 1091..1110 /label=T7 TE misc_feature 1117..1117 /label=stop polyA_site 1111..1124 /label=polya misc_feature 1084..1124 /label=BBa_B0012 terminator 1084..1124 /label=BBa_B0012 terminator 996..1124 /label=BBa_B0015 misc_feature 1133..1152 /label=LuxR/HSL promoter 1152..1157 /label=-35 promoter 1174..1179 /label=-10 misc_feature 1185..1185 /label=start misc_feature 1133..1187 /label=BBa_R0062 promoter 1133..1187 /label=BBa_R0062 CDS 1197..1813 /label=tetR misc_feature 1814..1847 /label=SsrA misc_feature 1854..1878 /label=Help:Barcodes CDS 1194..1878 /label=BBa_C0040 RBS 1887..1901 /label=RBS-1\Strong RBS 1894..1898 /label=RBS misc_feature 1887..1901 /label=BBa_B0030 RBS 1887..1901 /label=BBa_B0030 CDS 1908..2627 /label=GFP protein CDS 1908..2627 /label=BBa_E0040 misc_feature 2636..2674 /label=B1006 stem_loop 2645..2666 /label=modified thr terminator polyA_site 2667..2674 /label=PolyA polyA_site 2636..2644 /label=PolyA terminator 2636..2674 /label=BBa_B1006 misc_feature 1887..2674 /label=BBa_K081012 misc_feature 2683..2701 /label=TetR 1 misc_feature 2683..2736 /label=BBa_R0040 promoter 2702..2707 /label=-35 misc_feature 2708..2726 /label=TetR 2 promoter 2725..2730 /label=-10 promoter 2683..2736 /label=BBa_R0040 CDS 2743..3345 /label=LasI misc_feature 3346..3378 /label=LVA misc_feature 3385..3409 /label=Help:Barcodes CDS 2743..3409 /label=BBa_C0078 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagatga aaaacataaa tgccgacgac acatacagaa 241 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata 301 tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg 361 ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa tattatgatg 421 acgctaattt aataaaatat gatcctatag tagattattc taactccaat cattcaccaa 481 ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag 541 aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg 601 gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt 661 tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa 721 taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag 781 cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt tgcagtgagc 841 gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc 901 aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaat 961 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc 1021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1081 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 1141 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagatgtcca 1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1861 agtgctagtg tagatcacta ctagagatta aagaggagaa atactagatg cgtaaaggag 1921 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 1981 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta 2041 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg 2101 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca 2161 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga 2221 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt 2281 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact 2341 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact 2401 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa 2461 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat 2521 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa 2581 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagaaaaa 2641 aaaaccccgc ccctgacagg gcggggtttt tttttactag agtccctatc agtgatagag 2701 attgacatcc ctatcagtga tagagatact gagcactact agatgatcgt tcagatcggt 2761 cgtcgtgaag agttcgacaa aaaactgctg ggtgaaatgc acaaactgcg tgctcaggtt 2821 ttcaaagaac gtaaaggttg ggacgtttcc gttatcgacg aaatggaaat cgacggttac 2881 gacgctctgt ccccgtacta catgctgatc caggaagaca ccccggaagc tcaggttttc 2941 ggttgctggc gtatcttcga caccaccggt ccgtacatgc tgaaaaacac cttcccggaa 3001 ctgctgcacg gtaaagaagc tccgtgctcc ccgcacatct gggaactgtc ccgtttcgct 3061 atcaactccg gtcagaaagg ttccctgggt ttctccgact gcaccctgga agctatgcgt 3121 gctctggctc gttactcctt gcagaacgac atccagaccc tggttaccgt taccaccgtt 3181 ggtgttgaaa aaatgatgat ccgtgctggt ctggacgttt cccgtttcgg tccgcacctg 3241 aaaatcggta tcgaacgtgc tgttgctctg cgtatcgaac tgaacgctaa aacccagatc 3301 gctctgtacg gtggtgttct ggttgaacag cgtctggctg tttccgctgc taacgacgaa 3361 aactacgctc tggttgctta ataactctga tagtgctagt gtagatctc //