##gff-version 3
##sequence-region BBa_K876008 1 3409
BBa_K876008	.	CDS	2743	3409	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_K876008	.	engineered_tag	3385	3409	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K876008	.	CDS	2743	3345	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K876008	.	tag	3346	3378	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K876008	.	promoter	1133	1187	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K876008	.	promoter	1152	1157	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K876008	.	non_covalent_binding_site	1133	1152	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K876008	.	start_codon	1185	1185	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K876008	.	engineered_region	1133	1187	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K876008	.	promoter	1174	1179	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K876008	.	terminator	996	1124	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876008	.	terminator	996	1075	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876008	.	stem_loop	1007	1050	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876008	.	engineered_region	996	1075	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876008	.	terminator	1084	1124	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876008	.	stem_loop	1091	1110	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876008	.	polyA_site	1111	1124	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876008	.	stop_codon	1117	1117	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876008	.	engineered_region	1084	1124	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876008	.	promoter	2683	2736	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K876008	.	promoter	2702	2707	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K876008	.	engineered_region	2683	2736	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K876008	.	non_covalent_binding_site	2683	2701	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K876008	.	non_covalent_binding_site	2708	2726	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K876008	.	promoter	2725	2730	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K876008	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K876008	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K876008	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K876008	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K876008	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K876008	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K876008	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K876008	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K876008	.	CDS	207	962	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K876008	.	engineered_region	207	208	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K876008	.	engineered_tag	963	987	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K876008	.	point_mutation	698	698	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K876008	.	point_mutation	380	380	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K876008	.	engineered_region	207	962	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K876008	.	CDS	207	956	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K876008	.	engineered_region	1887	2674	.	+	0	ID=BBa_K081012;Name=BBa_K081012
BBa_K876008	.	terminator	2636	2674	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081012
BBa_K876008	.	polyA_site	2636	2644	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K876008	.	polyA_site	2667	2674	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K876008	.	engineered_region	2636	2674	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K876008	.	stem_loop	2645	2666	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K876008	.	CDS	1908	2627	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K081012
BBa_K876008	.	CDS	1908	2627	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K876008	.	ribosome_entry_site	1887	1901	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081012
BBa_K876008	.	ribosome_entry_site	1887	1901	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K876008	.	ribosome_entry_site	1894	1898	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K876008	.	engineered_region	1887	1901	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K876008	.	CDS	1194	1878	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K876008	.	tag	1814	1847	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K876008	.	CDS	1197	1813	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K876008	.	engineered_tag	1854	1878	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_K876008
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgaaaaacataaatgccgacgacacataca
gaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtaca
ttgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccct
aaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcatt
caccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatc
aggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaa
aaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatt
atcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatg
cgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcg
caaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatact
ttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaata
aatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaagg
tttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggct
ttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaag
attttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtac
acggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaat
gcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaag
aagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgc
agagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagattaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaataataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagtccc
tatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgatcgttcagatcggtcgtcg
tgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggac
gtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccc
cggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaact
gctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggt
tccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccc
tggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacct
gaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgtt
ctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgct
agtgtagatctc