LOCUS BBa_K876011 3941 bp DNA linear 04-May-2024 DEFINITION pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI ACCESSION BBa_K876011 VERSION BBa_K876011.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 CDS 61..777 /label=lasR misc_feature 778..810 /label=LVA misc_feature 540..540 /label=G misc_feature 817..841 /label=Help:Barcodes CDS 61..841 /label=BBa_C0079 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 terminator 850..978 /label=BBa_B0015 misc_feature 1032..1049 /label=OP2 misc_feature 1092..1111 /label=OP1 promoter 1103..1108 /label=-35 promoter 1126..1131 /label=-10 promoter 987..1143 /label=BBa_R0079 misc_feature 2239..2277 /label=LVA CDS 1150..2277 /label=lacI-LVA misc_feature 1150..2277 /label=BBa_C0012 misc_feature 2278..2302 /label=Help:Barcodes CDS 1150..2277 /label=BBa_C0012 RBS 2311..2325 /label=RBS-1\Strong RBS 2318..2322 /label=RBS misc_feature 2311..2325 /label=BBa_B0030 RBS 2311..2325 /label=BBa_B0030 CDS 2332..3006 /label=mrfp1 misc_feature 3013..3037 /label=Help:Barcodes CDS 2332..3037 /label=BBa_E1010 misc_feature 3046..3084 /label=B1006 stem_loop 3055..3076 /label=modified thr terminator polyA_site 3077..3084 /label=PolyA polyA_site 3046..3054 /label=PolyA terminator 3046..3084 /label=BBa_B1006 misc_feature 2311..3084 /label=BBa_K081014 CDS 3093..3180 /label=end of LacI coding region (inactive) misc_feature 3093..3292 /label=BBa_R0010 misc_feature 3181..3218 /label=CAP binding site promoter 3229..3234 /label=-35 promoter 3253..3258 /label=-10 misc_feature 3258..3292 /label=LacI binding site misc_feature 3265..3265 /label=start promoter 3093..3292 /label=BBa_R0010 misc_feature 3878..3909 /label=LVA CDS 3299..3877 /label=luxI misc_feature 3299..3916 /label=BBa_C0061 misc_feature 3917..3941 /label=Help:Barcodes CDS 3299..3916 /label=BBa_C0061 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc 121 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg 181 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg 241 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc 301 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag 361 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt 421 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc 481 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa 541 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg 601 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc 661 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt 721 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctcgct 781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagaggccc ctcgctgagc gcgtcccgga gctgggggca 1021 acctagctgc cacctgcttt tctgctagct attccagcga aaacatacag atttccggcg 1081 aaatcaaggc tacctgccag ttctggcagg tttggccgcg ggttcttttt ggtacacgaa 1141 agctactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 1201 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 1261 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 1321 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 1381 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 1441 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 1501 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 1561 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 1621 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 1681 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 1741 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 1801 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 1861 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 1921 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 1981 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 2041 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 2101 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 2161 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 2221 cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 2281 tgatagtgct agtgtagatc tctactagag attaaagagg agaaatacta gatggcttcc 2341 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac 2401 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc 2461 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg 2521 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg 2581 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt 2641 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa 2701 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg 2761 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg 2821 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct 2881 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc 2941 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc 3001 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagaaaaa aaaaccccgc 3061 ccctgacagg gcggggtttt tttttactag agcaatacgc aaaccgcctc tccccgcgcg 3121 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 3181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 3241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagat 3301 gactataatg ataaaaaaat cggatttttt ggcaattcca tcggaggagt ataaaggtat 3361 tctaagtctt cgttatcaag tgtttaagca aagacttgag tgggacttag ttgtagaaaa 3421 taaccttgaa tcagatgagt atgataactc aaatgcagaa tatatttatg cttgtgatga 3481 tactgaaaat gtaagtggat gctggcgttt attacctaca acaggtgatt atatgctgaa 3541 aagtgttttt cctgaattgc ttggtcaaca gagtgctccc aaagatccta atatagtcga 3601 attaagtcgt tttgctgtag gtaaaaatag ctcaaagata aataactctg ctagtgaaat 3661 tacaatgaaa ctatttgaag ctatatataa acacgctgtt agtcaaggta ttacagaata 3721 tgtaacagta acatcaacag caatagagcg atttttaaag cgtattaaag ttccttgtca 3781 tcgtattgga gacaaagaaa ttcatgtatt aggtgatact aaatcggttg tattgtctat 3841 gcctattaat gaacagttta aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc 3901 tttagtagct taataactct gatagtgcta gtgtagatct c //