##gff-version 3
##sequence-region BBa_K876011 1 3916
BBa_K876011	.	engineered_region	2311	3084	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K876011	.	terminator	3046	3084	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K876011	.	polyA_site	3046	3054	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K876011	.	polyA_site	3077	3084	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K876011	.	engineered_region	3046	3084	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K876011	.	stem_loop	3055	3076	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K876011	.	ribosome_entry_site	2311	2325	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K876011	.	engineered_region	2311	2325	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K876011	.	ribosome_entry_site	2311	2325	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K876011	.	ribosome_entry_site	2318	2322	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K876011	.	CDS	2332	3037	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K876011	.	CDS	2332	3006	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K876011	.	engineered_tag	3013	3037	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K876011	.	CDS	3299	3916	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K876011	.	engineered_region	3299	3916	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K876011	.	engineered_tag	3917	3941	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K876011	.	tag	3878	3909	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K876011	.	CDS	3299	3877	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K876011	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K876011	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K876011	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K876011	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K876011	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K876011	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K876011	.	CDS	1150	2277	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K876011	.	engineered_tag	2278	2302	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K876011	.	CDS	1150	2277	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K876011	.	tag	2239	2277	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K876011	.	engineered_region	1150	2277	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K876011	.	promoter	3093	3292	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K876011	.	non_covalent_binding_site	3181	3218	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K876011	.	engineered_region	3093	3292	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K876011	.	non_covalent_binding_site	3258	3292	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K876011	.	start_codon	3265	3265	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K876011	.	promoter	3229	3234	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K876011	.	CDS	3093	3180	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K876011	.	promoter	3253	3258	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K876011	.	terminator	850	978	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876011	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876011	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876011	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876011	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876011	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876011	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876011	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876011	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876011	.	CDS	61	841	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_K876011	.	engineered_tag	817	841	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K876011	.	tag	778	810	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K876011	.	sequence_alteration	540	540	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K876011	.	CDS	61	777	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K876011	.	promoter	987	1143	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K876011	.	non_covalent_binding_site	1092	1111	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K876011	.	promoter	1103	1108	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K876011	.	non_covalent_binding_site	1032	1049	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K876011	.	promoter	1126	1131	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
>BBa_K876011
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccttggttgacggtt
ttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctc
gaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcc
tggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccga
ttttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgta
tgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggcc
gaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggac
tggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcgg
caagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcgg
aagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagc
tgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctgg
caggtttggccgcgggttctttttggtacacgaaagctactagatggtgaatgtgaaaccagtaacgttatacgatgtc
gcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggg
aaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgtt
gctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgat
caactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttc
tcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcac
taatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacg
cgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcgg
cgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactg
gagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaac
gatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggat
acgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaac
cagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa
agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac
aggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgcta
gtgtagatctctactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgt
ttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaag
gtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttcca
gtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaa
tgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttca
tctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagc
ttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggt
cactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatca
aactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccgg
tgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacagggcggggttttt
ttttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttccc
gactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacacttta
tgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgactataatgataaaaaa
atcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagactt
gagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtg
atgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctga
attgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctca
aagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtatta
cagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggaga
caaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtc
ttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc