LOCUS       BBa_K876012             2955 bp    DNA     linear       22-Dec-2024
DEFINITION  pLas-LacI-RFP-ST-pLac-LuxI
ACCESSION   BBa_K876012
VERSION     BBa_K876012.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    1253..1291
                     /label=LVA
     CDS             164..1291
                     /label=lacI-LVA
     misc_feature    164..1291
                     /label=BBa_C0012
     misc_feature    1292..1316
                     /label=Help:Barcodes
     CDS             164..1291
                     /label=BBa_C0012
     RBS             1325..1339
                     /label=RBS-1\Strong
     RBS             1332..1336
                     /label=RBS
     misc_feature    1325..1339
                     /label=BBa_B0030
     RBS             1325..1339
                     /label=BBa_B0030
     CDS             1346..2020
                     /label=mrfp1
     misc_feature    2027..2051
                     /label=Help:Barcodes
     CDS             1346..2051
                     /label=BBa_E1010
     misc_feature    2060..2098
                     /label=B1006
     stem_loop       2069..2090
                     /label=modified thr terminator
     polyA_site      2091..2098
                     /label=PolyA
     polyA_site      2060..2068
                     /label=PolyA
     terminator      2060..2098
                     /label=BBa_B1006
     misc_feature    1325..2098
                     /label=BBa_K081014
     CDS             2107..2194
                     /label=end of LacI coding region (inactive)
     misc_feature    2107..2306
                     /label=BBa_R0010
     misc_feature    2195..2232
                     /label=CAP binding site
     promoter        2243..2248
                     /label=-35
     promoter        2267..2272
                     /label=-10
     misc_feature    2272..2306
                     /label=LacI binding site
     misc_feature    2279..2279
                     /label=start
     promoter        2107..2306
                     /label=BBa_R0010
     misc_feature    2892..2923
                     /label=LVA
     CDS             2313..2891
                     /label=luxI
     misc_feature    2313..2930
                     /label=BBa_C0061
     misc_feature    2931..2955
                     /label=Help:Barcodes
     CDS             2313..2930
                     /label=BBa_C0061
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagatggtga atgtgaaacc
      181 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      241 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      301 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      361 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      421 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      481 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      541 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      601 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      661 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
      721 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
      781 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
      841 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
      901 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
      961 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1021 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1081 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1141 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1201 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa
     1261 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact
     1321 agagattaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
     1381 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
     1441 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
     1501 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
     1561 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
     1621 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
     1681 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
     1741 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
     1801 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
     1861 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
     1921 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
     1981 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
     2041 gtgtagatcg ctactagaga aaaaaaaacc ccgcccctga cagggcgggg ttttttttta
     2101 ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2161 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     2221 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     2281 ttgtgagcgg ataacaattt cacacatact agatgactat aatgataaaa aaatcggatt
     2341 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta
     2401 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata
     2461 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc
     2521 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc
     2581 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa
     2641 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat
     2701 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag
     2761 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg
     2821 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag
     2881 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     2941 gctagtgtag atctc
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