LOCUS       BBa_K876013             2792 bp    DNA     linear       22-Nov-2024
DEFINITION  LacI-RFP-ST-pLac-LuxI
ACCESSION   BBa_K876013
VERSION     BBa_K876013.1  
FEATURES             Location/Qualifiers
     misc_feature    1090..1128
                     /label=LVA
     CDS             1..1128
                     /label=lacI-LVA
     misc_feature    1..1128
                     /label=BBa_C0012
     misc_feature    1129..1153
                     /label=Help:Barcodes
     CDS             1..1128
                     /label=BBa_C0012
     RBS             1162..1176
                     /label=RBS-1\Strong
     RBS             1169..1173
                     /label=RBS
     misc_feature    1162..1176
                     /label=BBa_B0030
     RBS             1162..1176
                     /label=BBa_B0030
     CDS             1183..1857
                     /label=mrfp1
     misc_feature    1864..1888
                     /label=Help:Barcodes
     CDS             1183..1888
                     /label=BBa_E1010
     misc_feature    1897..1935
                     /label=B1006
     stem_loop       1906..1927
                     /label=modified thr terminator
     polyA_site      1928..1935
                     /label=PolyA
     polyA_site      1897..1905
                     /label=PolyA
     terminator      1897..1935
                     /label=BBa_B1006
     misc_feature    1162..1935
                     /label=BBa_K081014
     CDS             1944..2031
                     /label=end of LacI coding region (inactive)
     misc_feature    1944..2143
                     /label=BBa_R0010
     misc_feature    2032..2069
                     /label=CAP binding site
     promoter        2080..2085
                     /label=-35
     promoter        2104..2109
                     /label=-10
     misc_feature    2109..2143
                     /label=LacI binding site
     misc_feature    2116..2116
                     /label=start
     promoter        1944..2143
                     /label=BBa_R0010
     misc_feature    2729..2760
                     /label=LVA
     CDS             2150..2728
                     /label=luxI
     misc_feature    2150..2767
                     /label=BBa_C0061
     misc_feature    2768..2792
                     /label=Help:Barcodes
     CDS             2150..2767
                     /label=BBa_C0061
ORIGIN
        1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
       61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
      961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1141 tagtgtagat ctctactaga gattaaagag gagaaatact agatggcttc ctccgaagac
     1201 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
     1261 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
     1321 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
     1381 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
     1441 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
     1501 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
     1561 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
     1621 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
     1681 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
     1741 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
     1801 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
     1861 taacgctgat agtgctagtg tagatcgcta ctagagaaaa aaaaaccccg cccctgacag
     1921 ggcggggttt ttttttacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat
     1981 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     2041 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     2101 tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga tgactataat
     2161 gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct
     2221 tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga
     2281 atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa
     2341 tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt
     2401 tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg
     2461 ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa
     2521 actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt
     2581 aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg
     2641 agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa
     2701 tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc
     2761 ttaataactc tgatagtgct agtgtagatc tc
//