LOCUS BBa_K876036 2822 bp DNA linear 22-Nov-2024 DEFINITION pTetR-LasR-TT-pLasR-RBS-CFP-TT-pLuxR-TetR ACCESSION BBa_K876036 VERSION BBa_K876036.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 CDS 61..777 /label=lasR misc_feature 778..810 /label=LVA misc_feature 540..540 /label=G misc_feature 817..841 /label=Help:Barcodes CDS 61..816 /label=BBa_C0079 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 terminator 850..978 /label=BBa_B0015 misc_feature 1032..1049 /label=OP2 misc_feature 1092..1111 /label=OP1 promoter 1103..1108 /label=-35 promoter 1126..1131 /label=-10 promoter 987..1143 /label=BBa_R0079 misc_feature 1156..1159 /label=conserved RBS 1152..1163 /label=BBa_B0034 CDS 1170..1931 /label=CFP (LVA) misc_feature 1888..1925 /label=SsrA misc_feature 1926..1931 /label=2 misc_feature 1238..1238 /label=A misc_feature 1170..1931 /label=BBa_E0022 misc_feature 1170..1931 /label=BBa_E0022 stem_loop 1951..1994 /label=stem_loop misc_feature 1940..2019 /label=BBa_B0010 terminator 1940..2019 /label=BBa_B0010 stem_loop 2035..2054 /label=T7 TE misc_feature 2061..2061 /label=stop polyA_site 2055..2068 /label=polya misc_feature 2028..2068 /label=BBa_B0012 terminator 2028..2068 /label=BBa_B0012 misc_feature 1152..2068 /label=BBa_E0422 misc_feature 2077..2096 /label=LuxR/HSL promoter 2096..2101 /label=-35 promoter 2118..2123 /label=-10 misc_feature 2129..2129 /label=start misc_feature 2077..2131 /label=BBa_R0062 promoter 2077..2131 /label=BBa_R0062 CDS 2141..2757 /label=tetR misc_feature 2758..2791 /label=SsrA misc_feature 2798..2822 /label=Help:Barcodes CDS 2138..2797 /label=BBa_C0040 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc 121 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg 181 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg 241 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc 301 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag 361 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt 421 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc 481 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa 541 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg 601 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc 661 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt 721 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctcgct 781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagaggccc ctcgctgagc gcgtcccgga gctgggggca 1021 acctagctgc cacctgcttt tctgctagct attccagcga aaacatacag atttccggcg 1081 aaatcaaggc tacctgccag ttctggcagg tttggccgcg ggttcttttt ggtacacgaa 1141 agctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 1201 gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt 1261 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 1321 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt 1381 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 1441 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 1501 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 1561 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg 1621 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca 1681 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 1741 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 1801 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 1861 ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag 1921 tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1981 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2041 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac 2101 gcaagaaaat ggtttgttat agtcgaataa atactagatg tccagattag ataaaagtaa 2161 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg 2221 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa 2281 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg 2341 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg 2401 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa 2461 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga 2521 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga 2581 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc 2641 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt 2701 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc 2761 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 2821 ac //