LOCUS       BBa_K876040             2495 bp    DNA     linear       17-May-2024
DEFINITION  TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR
ACCESSION   BBa_K876040
VERSION     BBa_K876040.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     CDS             301..816
                     /label=BBa_K091109
     misc_feature    829..832
                     /label=conserved
     RBS             825..836
                     /label=BBa_B0034
     CDS             843..1604
                     /label=CFP (LVA)
     misc_feature    1561..1598
                     /label=SsrA
     misc_feature    1599..1604
                     /label=2
     misc_feature    911..911
                     /label=A
     misc_feature    843..1604
                     /label=BBa_E0022
     misc_feature    843..1604
                     /label=BBa_E0022
     stem_loop       1624..1667
                     /label=stem_loop
     misc_feature    1613..1692
                     /label=BBa_B0010
     terminator      1613..1692
                     /label=BBa_B0010
     stem_loop       1708..1727
                     /label=T7 TE
     misc_feature    1734..1734
                     /label=stop
     polyA_site      1728..1741
                     /label=polya
     misc_feature    1701..1741
                     /label=BBa_B0012
     terminator      1701..1741
                     /label=BBa_B0012
     misc_feature    825..1741
                     /label=BBa_E0422
     misc_feature    1..1741
                     /label=BBa_K876039
     misc_feature    1750..1769
                     /label=LuxR/HSL
     promoter        1769..1774
                     /label=-35
     promoter        1791..1796
                     /label=-10
     misc_feature    1802..1802
                     /label=start
     misc_feature    1750..1804
                     /label=BBa_R0062
     promoter        1750..1804
                     /label=BBa_R0062
     CDS             1814..2430
                     /label=tetR
     misc_feature    2431..2464
                     /label=SsrA
     misc_feature    2471..2495
                     /label=Help:Barcodes
     CDS             1811..2495
                     /label=BBa_C0040
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 atgccgttgt tagatagctt cacagtcgat catacccgga tggaagcgcc tgctgttcgg
      361 gtggcgaaaa caatgaacac cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc
      421 tgcgtgccga acaaagaagt gatgccagaa agagggatcc ataccctgga gcacctgttt
      481 gctggtttta tgcgtaacca tcttaacggt aatggtgtag agattatcga tatctcgcca
      541 atgggctgcc gcaccggttt ttatatgagt ctgattggta cgccagatga gcagcgtgtt
      601 gctgatgcct ggaaagcggc aatggaagac gtgctgaaag tgcaggatca gaatcagatc
      661 ccggaactga acgtctacca gtgtggcact taccagatgc actcgttgca ggaagcgcag
      721 gatattgcgc gtagcattct ggaacgtgac gtacgcatca acagcaacga agaactggca
      781 ctgccgaaag agaagttgca ggaactgcac atctaatact agagaaagag gagaaatact
      841 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      901 acggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      961 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1021 ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga
     1081 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1141 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1201 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1261 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga
     1321 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1381 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1441 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     1501 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     1561 ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat
     1621 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1681 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1741 atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa
     1801 taaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct
     1861 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
     1921 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     1981 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     2041 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     2101 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     2161 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     2221 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     2281 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     2341 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     2401 agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt
     2461 agcttaataa cactgatagt gctagtgtag atcac
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