##gff-version 3
##sequence-region BBa_K876047 1 3570
BBa_K876047	.	engineered_region	2654	3570	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_K876047	.	engineered_region	2672	3433	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_K876047	.	tag	3389	3427	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_K876047	.	stop_codon	3428	3433	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_K876047	.	sequence_alteration	2913	2913	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_K876047	.	engineered_region	2672	3433	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_K876047	.	CDS	2672	3433	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_K876047	.	ribosome_entry_site	2654	2665	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_K876047	.	conserved_region	2658	2661	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K876047	.	terminator	3442	3521	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_K876047	.	engineered_region	3442	3521	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876047	.	stem_loop	3453	3496	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876047	.	terminator	3530	3570	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_K876047	.	stem_loop	3537	3556	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876047	.	stop_codon	3563	3563	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876047	.	engineered_region	3530	3570	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876047	.	polyA_site	3557	3570	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876047	.	promoter	1895	1996	.	+	0	ID=BBa_K117002;Name=BBa_K117002
BBa_K876047	.	ribosome_entry_site	2006	2017	.	+	0	ID=annotation1979372;Name=RBS BBa_B0034;Parent=BBa_K117002
BBa_K876047	.	engineered_region	1	1749	.	+	0	ID=BBa_K091159;Name=BBa_K091159
BBa_K876047	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091159
BBa_K876047	.	CDS	157	1749	.	+	0	ID=BBa_K091002;Name=BBa_K091002;Parent=BBa_K091159
BBa_K876047	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K091159
BBa_K876047	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K876047	.	terminator	1758	1886	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876047	.	terminator	1758	1837	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876047	.	engineered_region	1758	1837	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876047	.	stem_loop	1769	1812	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876047	.	terminator	1846	1886	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876047	.	stem_loop	1853	1872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876047	.	stop_codon	1879	1879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876047	.	engineered_region	1846	1886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876047	.	polyA_site	1873	1886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876047	.	CDS	2003	2620	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K876047	.	CDS	2003	2581	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K876047	.	tag	2582	2613	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K876047	.	engineered_region	2003	2620	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K876047	.	engineered_tag	2621	2645	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
>BBa_K876047
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggctcgactctttaccctttcagaatcaaagtactacctgatggcgctggatgcaggcaccggaagtattcgggctgtg
atattcgacctggaaggcaatcaaatagcagtgggacaggcggagtggcggcatctggcagtaccggacgttcctggtt
ctatggaatttgatctcaacaaaaactggcaactggcgtgtgagtgtatgcgccaggcgctgcacaacgccggcatagc
cccggagtatatcgctgccgtttcggcatgttcgatgcgtgaaggcattgttttatataataatgaaggagccccgatc
tgggcctgcgccaatgtggatgccagagcggcacgcgaagttagcgaacttaaagaactgcacaacaatacctttgaaa
acgaagtttatcgcgcgaccggacaaacactggctttaagtgccatccccagattactttggctggcgcaccatcgttc
cgatatttaccgtcaggcatcaaccatcaccatgatcagcgactggctggcctatatgctcagcggcgaactggcggtg
gatccctctaacgctggcaccacgggacttcttgatctaaccacccgtgactggaaacctgcattgctggatatggctg
gcctacgtgccgatattctttctcctgtcaaagaaaccggcacattgctgggcgtggtaagttcacaagcggcggaact
ctgcggtctgaaggcgggcactccggtggtcgttggaggaggcgacgtgcagcttggttgccttgggttaggcgttgtg
cgtccggcacaaaccgcggttcttggcggcacattctggcagcaagttgtaaatttagccgcgccggtgacagacccag
aaatgaacgtgcgcgttaatcctcatgttattcctggcatggtacaagctgaatctataagcttttttaccggactcac
catgcgctggttccgcgatgctttctgtgccgaagaaaaactgattgccgaacgtttaggcatcgacacctatacgctg
ctggaagagatggccagtcgggtgccgcctgggtcgtggggcgtaatgccgatcttctccgacagaatgcgctttaaaa
cctggtatcacgctgcgccttcctttattaacttgtccattgacccggataaatgtaacaaagcgacattgttccgtgc
gctggaagaaaatgcggcgattgtatcagcgtgtaacttgcagcaaattgctgatttctcgaatattcatccttcatcg
ttagtctttgcaggcggaggttcaaaagggaaattatggagtcaaattctcgctgatgtctcgggattacccgtcaata
ttccggtggtcaaagaagccactgcattaggatgtgccattgcagctggcgtcggtgccggaattttttcatcaatggc
agaaaccggagaacgcctggttcgctgggaacggacgcacacaccagacccggaaaagcatgaactttatcaggattca
cgcgataagtggcaggcagtttatcaggatcagctggggctggttgatcatggactgacgacgtcgttatggaaagcgc
ctgggttatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagca
caacatcaactgagtgcatgccttatttaatcatgtaactattcttacgacttcaaacgtcaaacagtcttaacactta
tttaattaaaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagt
ataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttga
atcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgt
ttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatc
ctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaat
gaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaata
gagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaat
cggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgcttt
agtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggc
gaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccg
gcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctg
gcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttc
ttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcg
ccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacat
cctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaag
gtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcg
gcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcg
cgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgct
gcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata