LOCUS       BBa_K876052             1731 bp    DNA     linear       25-Apr-2024
DEFINITION  plasR-luxI-RBS-YFP-Term-Term
ACCESSION   BBa_K876052
VERSION     BBa_K876052.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    743..774
                     /label=LVA
     CDS             164..742
                     /label=luxI
     misc_feature    164..781
                     /label=BBa_C0061
     misc_feature    782..806
                     /label=Help:Barcodes
     CDS             164..781
                     /label=BBa_C0061
     misc_feature    819..822
                     /label=conserved
     RBS             815..826
                     /label=BBa_B0034
     CDS             833..1594
                     /label=YFP (LVA)
     misc_feature    1589..1594
                     /label=2
     misc_feature    1550..1588
                     /label=SsrA
     misc_feature    1074..1074
                     /label=A (Q->L)
     misc_feature    833..1594
                     /label=BBa_E0032
     misc_feature    833..1594
                     /label=BBa_E0032
     stem_loop       1614..1657
                     /label=stem_loop
     misc_feature    1603..1682
                     /label=BBa_B0010
     terminator      1603..1682
                     /label=BBa_B0010
     stem_loop       1698..1717
                     /label=T7 TE
     misc_feature    1724..1724
                     /label=stop
     polyA_site      1718..1731
                     /label=polya
     misc_feature    1691..1731
                     /label=BBa_B0012
     terminator      1691..1731
                     /label=BBa_B0012
     misc_feature    815..1731
                     /label=BBa_E0432
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagatgacta taatgataaa
      181 aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta
      241 tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga
      301 tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag
      361 tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga
      421 attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc
      481 tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt
      541 tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc
      601 aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa
      661 agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca
      721 gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata
      781 actctgatag tgctagtgta gatctctact agagaaagag gagaaatact agatggtgag
      841 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      901 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      961 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1021 caccttcggc tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga
     1081 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1141 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1201 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1261 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1321 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1381 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1441 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1501 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctgctgc
     1561 aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat caaataaaac
     1621 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1681 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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