LOCUS BBa_K876052 1731 bp DNA linear 22-Nov-2024 DEFINITION plasR-luxI-RBS-YFP-Term-Term ACCESSION BBa_K876052 VERSION BBa_K876052.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 743..774 /label=LVA CDS 164..742 /label=luxI misc_feature 164..781 /label=BBa_C0061 misc_feature 782..806 /label=Help:Barcodes CDS 164..781 /label=BBa_C0061 misc_feature 819..822 /label=conserved RBS 815..826 /label=BBa_B0034 CDS 833..1594 /label=YFP (LVA) misc_feature 1589..1594 /label=2 misc_feature 1550..1588 /label=SsrA misc_feature 1074..1074 /label=A (Q->L) misc_feature 833..1594 /label=BBa_E0032 misc_feature 833..1594 /label=BBa_E0032 stem_loop 1614..1657 /label=stem_loop misc_feature 1603..1682 /label=BBa_B0010 terminator 1603..1682 /label=BBa_B0010 stem_loop 1698..1717 /label=T7 TE misc_feature 1724..1724 /label=stop polyA_site 1718..1731 /label=polya misc_feature 1691..1731 /label=BBa_B0012 terminator 1691..1731 /label=BBa_B0012 misc_feature 815..1731 /label=BBa_E0432 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagatgacta taatgataaa 181 aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta 241 tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga 301 tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag 361 tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga 421 attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc 481 tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt 541 tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc 601 aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa 661 agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca 721 gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata 781 actctgatag tgctagtgta gatctctact agagaaagag gagaaatact agatggtgag 841 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 901 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 961 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 1021 caccttcggc tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga 1081 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 1141 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 1201 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 1261 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 1321 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 1381 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 1441 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 1501 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctgctgc 1561 aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat caaataaaac 1621 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 1681 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //