LOCUS       BBa_K876057              978 bp    DNA     linear       22-Dec-2024
DEFINITION  pTetR-lasR-Term-Term
ACCESSION   BBa_K876057
VERSION     BBa_K876057.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             61..777
                     /label=lasR
     misc_feature    778..810
                     /label=LVA
     misc_feature    540..540
                     /label=G
     misc_feature    817..841
                     /label=Help:Barcodes
     CDS             61..841
                     /label=BBa_C0079
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     terminator      850..978
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc
      121 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg
      181 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg
      241 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc
      301 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag
      361 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt
      421 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc
      481 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa
      541 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg
      601 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc
      661 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt
      721 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctcgct
      781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttata
//