LOCUS       BBa_K876060             1219 bp    DNA     linear       22-Jan-2026
DEFINITION  Term-Term-plasR-RBS-CFP-Term-Term
ACCESSION   BBa_K876060
VERSION     BBa_K876060.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     misc_feature    307..310
                     /label=conserved
     RBS             303..314
                     /label=BBa_B0034
     CDS             321..1082
                     /label=CFP (LVA)
     misc_feature    1039..1076
                     /label=SsrA
     misc_feature    1077..1082
                     /label=2
     misc_feature    389..389
                     /label=A
     misc_feature    321..1082
                     /label=BBa_E0022
     misc_feature    321..1082
                     /label=BBa_E0022
     stem_loop       1102..1145
                     /label=stem_loop
     misc_feature    1091..1170
                     /label=BBa_B0010
     terminator      1091..1170
                     /label=BBa_B0010
     stem_loop       1186..1205
                     /label=T7 TE
     misc_feature    1212..1212
                     /label=stop
     polyA_site      1206..1219
                     /label=polya
     misc_feature    1179..1219
                     /label=BBa_B0012
     terminator      1179..1219
                     /label=BBa_B0012
     misc_feature    303..1219
                     /label=BBa_E0422
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      361 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      421 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      481 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      541 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      601 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      661 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      721 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      781 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      841 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      901 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      961 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1021 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat
     1081 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1141 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1201 gggcctttct gcgtttata
//