##gff-version 3 ##sequence-region BBa_K876060 1 1219 BBa_K876060 . engineered_region 303 1219 . + 0 ID=BBa_E0422;Name=BBa_E0422 BBa_K876060 . engineered_region 321 1082 . + 0 ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422 BBa_K876060 . tag 1039 1076 . + 0 ID=annotation2153;Name=SsrA;Parent=BBa_E0022 BBa_K876060 . CDS 321 1082 . + 0 ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022 BBa_K876060 . engineered_region 321 1082 . + 0 ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022 BBa_K876060 . point_mutation 389 389 . + 0 ID=annotation2155;Name=A;Parent=BBa_E0022 BBa_K876060 . stop_codon 1077 1082 . + 0 ID=annotation2154;Name=2;Parent=BBa_E0022 BBa_K876060 . terminator 1091 1170 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422 BBa_K876060 . stem_loop 1102 1145 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K876060 . engineered_region 1091 1170 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K876060 . terminator 1179 1219 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422 BBa_K876060 . engineered_region 1179 1219 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K876060 . stop_codon 1212 1212 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K876060 . polyA_site 1206 1219 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K876060 . stem_loop 1186 1205 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K876060 . ribosome_entry_site 303 314 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422 BBa_K876060 . conserved_region 307 310 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K876060 . terminator 1 129 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K876060 . terminator 1 80 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K876060 . stem_loop 12 55 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K876060 . engineered_region 1 80 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K876060 . terminator 89 129 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K876060 . engineered_region 89 129 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K876060 . stop_codon 122 122 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K876060 . polyA_site 116 129 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K876060 . stem_loop 96 115 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K876060 . promoter 138 294 . + 0 ID=BBa_R0079;Name=BBa_R0079 BBa_K876060 . promoter 277 282 . + 0 ID=annotation318496;Name=-10;Parent=BBa_R0079 BBa_K876060 . non_covalent_binding_site 243 262 . + 0 ID=annotation300992;Name=OP1;Parent=BBa_R0079 BBa_K876060 . non_covalent_binding_site 183 200 . + 0 ID=annotation300985;Name=OP2;Parent=BBa_R0079 BBa_K876060 . promoter 254 259 . + 0 ID=annotation318495;Name=-35;Parent=BBa_R0079 >BBa_K876060 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtccc ggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatca aggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaata ctagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggc cacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccg gcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgacca catgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgac ggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgact tcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaa gcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactac cagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagca aagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacga gctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaa acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact ggctcaccttcgggtgggcctttctgcgtttata