LOCUS       BBa_K876064             1676 bp    DNA     linear       22-Nov-2024
DEFINITION  plsrA-luxI-RBS-YFP-Term-term
ACCESSION   BBa_K876064
VERSION     BBa_K876064.1  
FEATURES             Location/Qualifiers
     RBS             112..123
                     /label=RBS BBa_B0034
     promoter        1..102
                     /label=BBa_K117002
     misc_feature    688..719
                     /label=LVA
     CDS             109..687
                     /label=luxI
     misc_feature    109..726
                     /label=BBa_C0061
     misc_feature    727..751
                     /label=Help:Barcodes
     CDS             109..726
                     /label=BBa_C0061
     misc_feature    764..767
                     /label=conserved
     RBS             760..771
                     /label=BBa_B0034
     CDS             778..1539
                     /label=YFP (LVA)
     misc_feature    1534..1539
                     /label=2
     misc_feature    1495..1533
                     /label=SsrA
     misc_feature    1019..1019
                     /label=A (Q->L)
     misc_feature    778..1539
                     /label=BBa_E0032
     misc_feature    778..1539
                     /label=BBa_E0032
     stem_loop       1559..1602
                     /label=stem_loop
     misc_feature    1548..1627
                     /label=BBa_B0010
     terminator      1548..1627
                     /label=BBa_B0010
     stem_loop       1643..1662
                     /label=T7 TE
     misc_feature    1669..1669
                     /label=stop
     polyA_site      1663..1676
                     /label=polya
     misc_feature    1636..1676
                     /label=BBa_B0012
     terminator      1636..1676
                     /label=BBa_B0012
     misc_feature    760..1676
                     /label=BBa_E0432
ORIGIN
        1 cacaacatca actgagtgca tgccttattt aatcatgtaa ctattcttac gacttcaaac
       61 gtcaaacagt cttaacactt atttaattaa aaagaggaga aatactagat gactataatg
      121 ataaaaaaat cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt
      181 cgttatcaag tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa
      241 tcagatgagt atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat
      301 gtaagtggat gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt
      361 cctgaattgc ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt
      421 tttgctgtag gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa
      481 ctatttgaag ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta
      541 acatcaacag caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga
      601 gacaaagaaa ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat
      661 gaacagttta aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct
      721 taataactct gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg
      781 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      841 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      901 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      961 gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg
     1021 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1081 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1141 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1201 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1261 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1321 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1381 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1441 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct
     1501 gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat
     1561 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1621 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//