##gff-version 3
##sequence-region BBa_K876065 1 2717
BBa_K876065	.	promoter	987	1143	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K876065	.	non_covalent_binding_site	1092	1111	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K876065	.	non_covalent_binding_site	1032	1049	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K876065	.	promoter	1103	1108	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K876065	.	promoter	1126	1131	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K876065	.	CDS	61	816	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K876065	.	engineered_tag	817	841	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K876065	.	engineered_region	61	816	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K876065	.	engineered_region	61	62	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K876065	.	point_mutation	234	234	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K876065	.	CDS	61	810	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K876065	.	point_mutation	552	552	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K876065	.	CDS	1150	1767	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K876065	.	CDS	1150	1728	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K876065	.	engineered_tag	1768	1792	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K876065	.	engineered_region	1150	1767	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K876065	.	tag	1729	1760	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K876065	.	terminator	850	978	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876065	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876065	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876065	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876065	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876065	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876065	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876065	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876065	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876065	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K876065	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K876065	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K876065	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K876065	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K876065	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K876065	.	engineered_region	1801	2717	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_K876065	.	terminator	2677	2717	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_K876065	.	stop_codon	2710	2710	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876065	.	polyA_site	2704	2717	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876065	.	stem_loop	2684	2703	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876065	.	engineered_region	2677	2717	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876065	.	ribosome_entry_site	1801	1812	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_K876065	.	conserved_region	1805	1808	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K876065	.	engineered_region	1819	2580	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_K876065	.	stop_codon	2575	2580	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_K876065	.	tag	2536	2574	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_K876065	.	engineered_region	1819	2580	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_K876065	.	sequence_alteration	2060	2060	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_K876065	.	CDS	1819	2580	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_K876065	.	terminator	2589	2668	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_K876065	.	engineered_region	2589	2668	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876065	.	stem_loop	2600	2643	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K876065
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgaaaaacataaatgccg
acgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgac
taaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatccta
gataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattcta
actccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaaga
agcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagtttt
gcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctc
tagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgttt
agcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccat
ttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattg
attgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagc
tgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctgg
caggtttggccgcgggttctttttggtacacgaaagctactagatgactataatgataaaaaaatcggattttttggca
attccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttg
tagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgt
aagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacag
agtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctg
ctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagt
aacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgta
ttaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacg
acgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacat
gaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagca
gaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata