LOCUS BBa_K876066 5126 bp DNA linear 25-Nov-2024 DEFINITION plac-luxR-Term-Term-pluxR-araC-lasR-Term-Term-pBad-tetR-Term-Term-p lasR.TetO-RBS-CFP-Term-Term ACCESSION BBa_K876066 VERSION BBa_K876066.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 207..208 /label=prefix misc_feature 380..380 /label=T misc_feature 698..698 /label=A CDS 207..956 /label=luxR misc_feature 207..962 /label=BBa_C0062 misc_feature 963..987 /label=Help:Barcodes CDS 207..962 /label=BBa_C0062 stem_loop 1007..1050 /label=stem_loop misc_feature 996..1075 /label=BBa_B0010 terminator 996..1075 /label=BBa_B0010 stem_loop 1091..1110 /label=T7 TE misc_feature 1117..1117 /label=stop polyA_site 1111..1124 /label=polya misc_feature 1084..1124 /label=BBa_B0012 terminator 1084..1124 /label=BBa_B0012 terminator 996..1124 /label=BBa_B0015 misc_feature 1133..1152 /label=LuxR/HSL promoter 1152..1157 /label=-35 promoter 1174..1179 /label=-10 misc_feature 1185..1185 /label=start misc_feature 1133..1187 /label=BBa_R0062 promoter 1133..1187 /label=BBa_R0062 CDS 1194..2069 /label=araC misc_feature 2070..2102 /label=LVA misc_feature 2109..2133 /label=Help:Barcodes CDS 1194..2133 /label=BBa_C0080 CDS 2140..2856 /label=lasR misc_feature 2857..2889 /label=LVA misc_feature 2619..2619 /label=G misc_feature 2896..2920 /label=Help:Barcodes CDS 2140..2920 /label=BBa_C0079 stem_loop 2940..2983 /label=stem_loop misc_feature 2929..3008 /label=BBa_B0010 terminator 2929..3008 /label=BBa_B0010 stem_loop 3024..3043 /label=T7 TE misc_feature 3050..3050 /label=stop polyA_site 3044..3057 /label=polya misc_feature 3017..3057 /label=BBa_B0012 terminator 3017..3057 /label=BBa_B0012 terminator 2929..3057 /label=BBa_B0015 promoter 3066..3195 /label=BBa_I13453 CDS 3205..3821 /label=tetR misc_feature 3822..3855 /label=SsrA misc_feature 3862..3886 /label=Help:Barcodes CDS 3202..3886 /label=BBa_C0040 stem_loop 3906..3949 /label=stem_loop misc_feature 3895..3974 /label=BBa_B0010 terminator 3895..3974 /label=BBa_B0010 stem_loop 3990..4009 /label=T7 TE misc_feature 4016..4016 /label=stop polyA_site 4010..4023 /label=polya misc_feature 3983..4023 /label=BBa_B0012 terminator 3983..4023 /label=BBa_B0012 terminator 3895..4023 /label=BBa_B0015 misc_feature 4032..4182 /label=R0079 misc_feature 4183..4201 /label=TetR promoter 4171..4176 /label=-10 promoter 4148..4153 /label=-35 promoter 4032..4201 /label=BBa_I14015 misc_feature 4214..4217 /label=conserved RBS 4210..4221 /label=BBa_B0034 CDS 4228..4989 /label=CFP (LVA) misc_feature 4946..4983 /label=SsrA misc_feature 4984..4989 /label=2 misc_feature 4296..4296 /label=A misc_feature 4228..4989 /label=BBa_E0022 misc_feature 4228..4989 /label=BBa_E0022 stem_loop 5009..5052 /label=stem_loop misc_feature 4998..5077 /label=BBa_B0010 terminator 4998..5077 /label=BBa_B0010 stem_loop 5093..5112 /label=T7 TE misc_feature 5119..5119 /label=stop polyA_site 5113..5126 /label=polya misc_feature 5086..5126 /label=BBa_B0012 terminator 5086..5126 /label=BBa_B0012 misc_feature 4210..5126 /label=BBa_E0422 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagatga aaaacataaa tgccgacgac acatacagaa 241 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata 301 tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg 361 ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa tattatgatg 421 acgctaattt aataaaatat gatcctatag tagattattc taactccaat cattcaccaa 481 ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag 541 aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg 601 gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt 661 tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa 721 taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag 781 cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt tgcagtgagc 841 gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc 901 aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaat 961 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc 1021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1081 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 1141 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagatggctg 1201 aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa cgcccatctg gtggcgggtt 1261 taacgccgat tgaggccaat ggttatctcg atttttttat cgaccgaccg ctgggaatga 1321 aaggttatat tctcaatctc accattcgcg gtcagggggt ggtgaaaaat cagggacgag 1381 aatttgtctg ccgaccgggt gatattttgc tgttcccgcc aggagagatt catcactacg 1441 gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt ttactttcgt ccgcgcgcct 1501 actggcatga atggcttaac tggccgtcaa tatttgccaa tacgggtttc tttcgcccgg 1561 atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaac gccgggcaag 1621 gggaagggcg ctattcggag ctgctggcga taaatctgct tgagcaattg ttactgcggc 1681 gcatggaagc gattaacgag tcgctccatc caccgatgga taatcgggta cgcgaggctt 1741 gtcagtacat cagcgatcac ctggcagaca gcaattttga tatcgccagc gtcgcacagc 1801 atgtttgcct gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta gggattagcg 1861 tcttaagctg gcgcgaggac caacgcatca gccaggcgaa gctgcttttg agcactaccc 1921 ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc tatttctcgc 1981 gagtatttaa aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa 2041 aagtgaatga tgtagccgtc aagttgtcag ctgcaaacga cgaaaactac gctttagtag 2101 cttaataaca ctgatagtgc tagtgtagat cactactaga tggccttggt tgacggtttt 2161 cttgagctgg aacgctcaag tggaaaattg gagtggagcg ccatcctcca gaagatggcg 2221 agcgaccttg gattctcgaa gatcctgttc ggcctgttgc ctaaggacag ccaggactac 2281 gagaacgcct tcatcgtcgg caactacccg gccgcctggc gcgagcatta cgaccgggct 2341 ggctacgcgc gggtcgaccc gacggtcagt cactgtaccc agagcgtact gccgattttc 2401 tgggaaccgt ccatctacca gacgcgaaag cagcacgagt tcttcgagga agcctcggcc 2461 gccggcctgg tgtatgggct gaccatgccg ctgcatggtg ctcgcggcga actcggcgcg 2521 ctgagcctca gcgtggaagc ggaaaaccgg gccgaggcca accgtttcat agagtcggtc 2581 ctgccgaccc tgtggatgct caaggactac gcactgcaaa gcggtgccgg actggccttc 2641 gaacatccgg tcagcaaacc ggtggttctg accagccggg agaaggaagt gttgcagtgg 2701 tgcgccatcg gcaagaccag ttgggagata tcggttatct gcaactgctc ggaagccaat 2761 gtgaacttcc atatgggaaa tattcggcgg aagttcggtg tgacctcccg ccgcgtagcg 2821 gccattatgg ccgttaattt gggtcttatt actctcgctg caaacgacga aaactacgct 2881 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa 2941 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3001 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 3061 tagagacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat 3121 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccg 3181 tttttttggg ctagctacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 3241 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 3301 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 3361 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 3421 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 3481 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 3541 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 3601 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 3661 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 3721 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 3781 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 3841 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 3901 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3961 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 4021 atatactaga ggcccctcgc tgagcgcgtc ccggagctgg gggcaaccta gctgccacct 4081 gcttttctgc tagctattcc agcgaaaaca tacagatttc cggcgaaatc aaggctacct 4141 gccagttctg gcaggtttgg ccgcgggttc tttttggtac actccctatc agtgatagag 4201 atactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg 4261 gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt cagcgtgtcc 4321 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4381 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc 4441 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4501 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 4561 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 4621 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc 4681 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac 4741 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 4801 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac 4861 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 4921 ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta 4981 gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 5041 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 5101 ttcgggtggg cctttctgcg tttata //