##gff-version 3
##sequence-region BBa_K876066 1 5126
BBa_K876066	.	promoter	4032	4201	.	+	0	ID=BBa_I14015;Name=BBa_I14015
BBa_K876066	.	promoter	4148	4153	.	+	0	ID=annotation1026120;Name=-35;Parent=BBa_I14015
BBa_K876066	.	non_covalent_binding_site	4183	4201	.	+	0	ID=annotation1017910;Name=TetR;Parent=BBa_I14015
BBa_K876066	.	promoter	4171	4176	.	+	0	ID=annotation1026116;Name=-10;Parent=BBa_I14015
BBa_K876066	.	engineered_region	4032	4182	.	+	0	ID=annotation1017909;Name=R0079;Parent=BBa_I14015
BBa_K876066	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K876066	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K876066	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K876066	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K876066	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K876066	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K876066	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K876066	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K876066	.	promoter	3066	3195	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K876066	.	CDS	3202	3886	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K876066	.	engineered_tag	3862	3886	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K876066	.	tag	3822	3855	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K876066	.	CDS	3205	3821	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K876066	.	CDS	2140	2920	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_K876066	.	tag	2857	2889	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K876066	.	sequence_alteration	2619	2619	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K876066	.	engineered_tag	2896	2920	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K876066	.	CDS	2140	2856	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K876066	.	terminator	2929	3057	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876066	.	terminator	2929	3008	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876066	.	stem_loop	2940	2983	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876066	.	engineered_region	2929	3008	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876066	.	terminator	3017	3057	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876066	.	engineered_region	3017	3057	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876066	.	stop_codon	3050	3050	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876066	.	stem_loop	3024	3043	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876066	.	polyA_site	3044	3057	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876066	.	terminator	996	1124	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876066	.	terminator	996	1075	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876066	.	stem_loop	1007	1050	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876066	.	engineered_region	996	1075	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876066	.	terminator	1084	1124	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876066	.	engineered_region	1084	1124	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876066	.	stop_codon	1117	1117	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876066	.	stem_loop	1091	1110	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876066	.	polyA_site	1111	1124	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876066	.	terminator	3895	4023	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K876066	.	terminator	3895	3974	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876066	.	stem_loop	3906	3949	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876066	.	engineered_region	3895	3974	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876066	.	terminator	3983	4023	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876066	.	engineered_region	3983	4023	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876066	.	stop_codon	4016	4016	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876066	.	stem_loop	3990	4009	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876066	.	polyA_site	4010	4023	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876066	.	promoter	1133	1187	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K876066	.	non_covalent_binding_site	1133	1152	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K876066	.	promoter	1174	1179	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K876066	.	engineered_region	1133	1187	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K876066	.	start_codon	1185	1185	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K876066	.	promoter	1152	1157	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K876066	.	CDS	207	962	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K876066	.	CDS	207	956	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K876066	.	engineered_region	207	962	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K876066	.	engineered_tag	963	987	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K876066	.	point_mutation	380	380	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K876066	.	engineered_region	207	208	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K876066	.	point_mutation	698	698	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K876066	.	engineered_region	4210	5126	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_K876066	.	engineered_region	4228	4989	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_K876066	.	CDS	4228	4989	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K876066	.	tag	4946	4983	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K876066	.	engineered_region	4228	4989	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K876066	.	point_mutation	4296	4296	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K876066	.	stop_codon	4984	4989	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K876066	.	terminator	4998	5077	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_K876066	.	stem_loop	5009	5052	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876066	.	engineered_region	4998	5077	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876066	.	terminator	5086	5126	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_K876066	.	engineered_region	5086	5126	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876066	.	stop_codon	5119	5119	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876066	.	stem_loop	5093	5112	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876066	.	polyA_site	5113	5126	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876066	.	ribosome_entry_site	4210	4221	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_K876066	.	conserved_region	4214	4217	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K876066	.	CDS	1194	2133	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_K876066	.	CDS	1194	2069	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K876066	.	tag	2070	2102	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K876066	.	engineered_tag	2109	2133	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
>BBa_K876066
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgaaaaacataaatgccgacgacacataca
gaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtaca
ttgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccct
aaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcatt
caccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatc
aggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaa
aaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatt
atcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatg
cgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcg
caaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatact
ttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaata
aatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaac
gccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcacc
attcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccag
gagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgccta
ctggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcat
ttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgc
ttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggc
ttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccg
tcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccagg
cgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctattt
ctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgta
gccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
tactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccaga
agatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgcctt
catcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagt
cactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgagg
aagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcct
cagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggac
tacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaagg
aagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaa
cttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggt
cttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgca
cggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactc
tctactgtttctccataccgtttttttgggctagctactagatgtccagattagataaaagtaaagtgattaacagcgc
attagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcct
acattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactc
acttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaag
tcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcc
tttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacg
acaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatta
gaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagt
gctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
aggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaa
catacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactccct
atcagtgatagagatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtg
cccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacct
acggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgac
ctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggc
tacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgaca
ccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaa
ctacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaac
atcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccg
acaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagtt
cgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgcttta
gtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata