LOCUS BBa_K876071 3640 bp DNA linear 15-Jun-2024 DEFINITION pLac-TetR-RBS-RFP-pTetR-LacI-RBS-GFP-Term ACCESSION BBa_K876071 VERSION BBa_K876071.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 CDS 210..826 /label=tetR misc_feature 827..860 /label=SsrA misc_feature 867..891 /label=Help:Barcodes CDS 207..891 /label=BBa_C0040 misc_feature 904..907 /label=conserved RBS 900..911 /label=BBa_B0034 CDS 918..1592 /label=mrfp1 misc_feature 1599..1623 /label=Help:Barcodes CDS 918..1623 /label=BBa_E1010 misc_feature 900..1623 /label=BBa_K093005 misc_feature 1632..1650 /label=TetR 1 misc_feature 1632..1685 /label=BBa_R0040 promoter 1651..1656 /label=-35 misc_feature 1657..1675 /label=TetR 2 promoter 1674..1679 /label=-10 promoter 1632..1685 /label=BBa_R0040 misc_feature 2781..2819 /label=LVA CDS 1692..2819 /label=lacI-LVA misc_feature 1692..2819 /label=BBa_C0012 misc_feature 2820..2844 /label=Help:Barcodes CDS 1692..2819 /label=BBa_C0012 RBS 2853..2867 /label=RBS-1\Strong RBS 2860..2864 /label=RBS misc_feature 2853..2867 /label=BBa_B0030 RBS 2853..2867 /label=BBa_B0030 CDS 2874..3593 /label=GFP protein CDS 2874..3593 /label=BBa_E0040 misc_feature 3602..3640 /label=B1006 stem_loop 3611..3632 /label=modified thr terminator polyA_site 3633..3640 /label=PolyA polyA_site 3602..3610 /label=PolyA terminator 3602..3640 /label=BBa_B1006 misc_feature 2853..3640 /label=BBa_K081012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagatgt ccagattaga taaaagtaaa gtgattaaca 241 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc 301 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc 361 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag 421 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa 481 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa 541 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat 601 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc 661 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac 721 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat 781 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg 841 aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca ctactagaga 901 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca 961 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag 1021 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc 1081 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac 1141 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac 1201 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag 1261 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg 1321 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg 1381 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg 1441 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa 1501 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg 1561 aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat 1621 cgctactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 1681 agcactacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 1741 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 1801 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 1861 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 1921 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 1981 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 2041 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 2101 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 2161 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 2221 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 2281 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 2341 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 2401 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 2461 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 2521 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 2581 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 2641 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 2701 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 2761 cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac 2821 tctgatagtg ctagtgtaga tctctactag agattaaaga ggagaaatac tagatgcgta 2881 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 2941 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 3001 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 3061 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact 3121 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg 3181 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa 3241 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat 3301 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag 3361 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 3421 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca 3481 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt 3541 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga 3601 gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt //