##gff-version 3
##sequence-region BBa_K876071 1 3640
BBa_K876071	.	engineered_region	900	1623	.	+	0	ID=BBa_K093005;Name=BBa_K093005
BBa_K876071	.	ribosome_entry_site	900	911	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_K876071	.	conserved_region	904	907	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K876071	.	CDS	918	1623	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_K876071	.	CDS	918	1592	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K876071	.	engineered_tag	1599	1623	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K876071	.	promoter	1632	1685	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K876071	.	non_covalent_binding_site	1657	1675	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K876071	.	non_covalent_binding_site	1632	1650	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K876071	.	engineered_region	1632	1685	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K876071	.	promoter	1651	1656	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K876071	.	promoter	1674	1679	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K876071	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K876071	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K876071	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K876071	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K876071	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K876071	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K876071	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K876071	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K876071	.	CDS	207	891	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K876071	.	engineered_tag	867	891	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K876071	.	CDS	210	826	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K876071	.	tag	827	860	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K876071	.	engineered_region	2853	3640	.	+	0	ID=BBa_K081012;Name=BBa_K081012
BBa_K876071	.	CDS	2874	3593	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K081012
BBa_K876071	.	CDS	2874	3593	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K876071	.	ribosome_entry_site	2853	2867	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081012
BBa_K876071	.	engineered_region	2853	2867	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K876071	.	ribosome_entry_site	2860	2864	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K876071	.	ribosome_entry_site	2853	2867	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K876071	.	terminator	3602	3640	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081012
BBa_K876071	.	polyA_site	3633	3640	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K876071	.	engineered_region	3602	3640	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K876071	.	polyA_site	3602	3610	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K876071	.	stem_loop	3611	3632	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K876071	.	CDS	1692	2819	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K876071	.	engineered_region	1692	2819	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K876071	.	CDS	1692	2819	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K876071	.	engineered_tag	2820	2844	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K876071	.	tag	2781	2819	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
>BBa_K876071
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgtccagattagataaaagtaaagtgatta
acagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtaga
gcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcac
catactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctt
tactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatca
attagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttta
ggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccat
tattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatg
cggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacac
tgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagt
tcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtcc
gtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaag
gtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacgg
tgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggt
tgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaag
acggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaac
cgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcac
tccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatcc
ctatcagtgatagagatactgagcactactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgc
cggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaa
gcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcg
ttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgc
cagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgc
gtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccgg
cgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgt
ggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgt
ctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgt
ccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggc
gctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatacc
gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggacc
gcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccac
cctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccga
ctggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
tactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagaaaaaaaaaccccgcccctgacagggcggggtt
tttttt