LOCUS       BBa_K876072             1623 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-TetR-RBS-RFP
ACCESSION   BBa_K876072
VERSION     BBa_K876072.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     CDS             210..826
                     /label=tetR
     misc_feature    827..860
                     /label=SsrA
     misc_feature    867..891
                     /label=Help:Barcodes
     CDS             207..891
                     /label=BBa_C0040
     misc_feature    904..907
                     /label=conserved
     RBS             900..911
                     /label=BBa_B0034
     CDS             918..1592
                     /label=mrfp1
     misc_feature    1599..1623
                     /label=Help:Barcodes
     CDS             918..1623
                     /label=BBa_E1010
     misc_feature    900..1623
                     /label=BBa_K093005
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatgt ccagattaga taaaagtaaa gtgattaaca
      241 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
      301 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
      361 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
      421 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
      481 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
      541 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
      601 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
      661 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
      721 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
      781 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg
      841 aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca ctactagaga
      901 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca
      961 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag
     1021 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc
     1081 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac
     1141 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac
     1201 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag
     1261 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg
     1321 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg
     1381 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg
     1441 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa
     1501 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg
     1561 aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat
     1621 cgc
//