LOCUS BBa_K876073 3344 bp DNA linear 29-Mar-2024 DEFINITION pTetR-LasR-Term-Term-pLasR-LuxS-RBS-CFP-Term-Term-pLuxR-TetR ACCESSION BBa_K876073 VERSION BBa_K876073.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 CDS 61..777 /label=lasR misc_feature 778..810 /label=LVA misc_feature 540..540 /label=G misc_feature 817..841 /label=Help:Barcodes CDS 61..841 /label=BBa_C0079 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 terminator 850..978 /label=BBa_B0015 misc_feature 1..978 /label=BBa_K876057 misc_feature 1032..1049 /label=OP2 misc_feature 1092..1111 /label=OP1 promoter 1103..1108 /label=-35 promoter 1126..1131 /label=-10 promoter 987..1143 /label=BBa_R0079 CDS 1150..1665 /label=BBa_K091109 misc_feature 1678..1681 /label=conserved RBS 1674..1685 /label=BBa_B0034 CDS 1692..2453 /label=CFP (LVA) misc_feature 2410..2447 /label=SsrA misc_feature 2448..2453 /label=2 misc_feature 1760..1760 /label=A misc_feature 1692..2453 /label=BBa_E0022 misc_feature 1692..2453 /label=BBa_E0022 stem_loop 2473..2516 /label=stem_loop misc_feature 2462..2541 /label=BBa_B0010 terminator 2462..2541 /label=BBa_B0010 stem_loop 2557..2576 /label=T7 TE misc_feature 2583..2583 /label=stop polyA_site 2577..2590 /label=polya misc_feature 2550..2590 /label=BBa_B0012 terminator 2550..2590 /label=BBa_B0012 misc_feature 1674..2590 /label=BBa_E0422 misc_feature 2599..2618 /label=LuxR/HSL promoter 2618..2623 /label=-35 promoter 2640..2645 /label=-10 misc_feature 2651..2651 /label=start misc_feature 2599..2653 /label=BBa_R0062 promoter 2599..2653 /label=BBa_R0062 CDS 2663..3279 /label=tetR misc_feature 3280..3313 /label=SsrA misc_feature 3320..3344 /label=Help:Barcodes CDS 2660..3344 /label=BBa_C0040 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc 121 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg 181 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg 241 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc 301 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag 361 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt 421 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc 481 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa 541 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg 601 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc 661 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt 721 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctcgct 781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagaggccc ctcgctgagc gcgtcccgga gctgggggca 1021 acctagctgc cacctgcttt tctgctagct attccagcga aaacatacag atttccggcg 1081 aaatcaaggc tacctgccag ttctggcagg tttggccgcg ggttcttttt ggtacacgaa 1141 agctactaga tgccgttgtt agatagcttc acagtcgatc atacccggat ggaagcgcct 1201 gctgttcggg tggcgaaaac aatgaacacc ccgcatggcg acgcaatcac cgtgttcgat 1261 ctgcgcttct gcgtgccgaa caaagaagtg atgccagaaa gagggatcca taccctggag 1321 cacctgtttg ctggttttat gcgtaaccat cttaacggta atggtgtaga gattatcgat 1381 atctcgccaa tgggctgccg caccggtttt tatatgagtc tgattggtac gccagatgag 1441 cagcgtgttg ctgatgcctg gaaagcggca atggaagacg tgctgaaagt gcaggatcag 1501 aatcagatcc cggaactgaa cgtctaccag tgtggcactt accagatgca ctcgttgcag 1561 gaagcgcagg atattgcgcg tagcattctg gaacgtgacg tacgcatcaa cagcaacgaa 1621 gaactggcac tgccgaaaga gaagttgcag gaactgcaca tctaatacta gagaaagagg 1681 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 1741 tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg 1801 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 1861 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg 1921 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 1981 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 2041 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 2101 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca 2161 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg 2221 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 2281 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 2341 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 2401 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga 2461 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2521 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2581 tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt 2641 atagtcgaat aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 2701 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 2761 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 2821 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 2881 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 2941 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 3001 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 3061 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 3121 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 3181 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 3241 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 3301 cgctttagta gcttaataac actgatagtg ctagtgtaga tcac //