##gff-version 3
##sequence-region BBa_K876073 1 3344
BBa_K876073	.	engineered_region	1	978	.	+	0	ID=BBa_K876057;Name=BBa_K876057
BBa_K876073	.	terminator	850	978	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K876057
BBa_K876073	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K876073	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876073	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876073	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K876073	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876073	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876073	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876073	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876073	.	CDS	61	841	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_K876057
BBa_K876073	.	tag	778	810	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K876073	.	CDS	61	777	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K876073	.	sequence_alteration	540	540	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K876073	.	engineered_tag	817	841	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K876073	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K876057
BBa_K876073	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K876073	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K876073	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K876073	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K876073	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K876073	.	CDS	2660	3344	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K876073	.	tag	3280	3313	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K876073	.	engineered_tag	3320	3344	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K876073	.	CDS	2663	3279	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K876073	.	promoter	987	1143	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K876073	.	promoter	1103	1108	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K876073	.	non_covalent_binding_site	1032	1049	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K876073	.	promoter	1126	1131	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K876073	.	non_covalent_binding_site	1092	1111	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K876073	.	engineered_region	1674	2590	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_K876073	.	terminator	2550	2590	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_K876073	.	engineered_region	2550	2590	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K876073	.	stop_codon	2583	2583	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K876073	.	polyA_site	2577	2590	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K876073	.	stem_loop	2557	2576	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K876073	.	engineered_region	1692	2453	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_K876073	.	CDS	1692	2453	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K876073	.	tag	2410	2447	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K876073	.	stop_codon	2448	2453	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K876073	.	engineered_region	1692	2453	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K876073	.	point_mutation	1760	1760	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K876073	.	terminator	2462	2541	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_K876073	.	engineered_region	2462	2541	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K876073	.	stem_loop	2473	2516	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K876073	.	ribosome_entry_site	1674	1685	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_K876073	.	conserved_region	1678	1681	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K876073	.	promoter	2599	2653	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K876073	.	non_covalent_binding_site	2599	2618	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K876073	.	promoter	2618	2623	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K876073	.	engineered_region	2599	2653	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K876073	.	promoter	2640	2645	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K876073	.	start_codon	2651	2651	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K876073	.	CDS	1150	1665	.	+	0	ID=BBa_K091109;Name=BBa_K091109
>BBa_K876073
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccttggttgacggtt
ttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctc
gaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcc
tggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccga
ttttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgta
tgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggcc
gaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggac
tggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcgg
caagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcgg
aagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagc
tgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctgg
caggtttggccgcgggttctttttggtacacgaaagctactagatgccgttgttagatagcttcacagtcgatcatacc
cggatggaagcgcctgctgttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgc
gcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcg
taaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctg
attggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcaga
atcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcg
tagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcac
atctaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcct
ggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaag
ctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcg
tgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtcca
ggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtg
aaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatca
gccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgagga
cggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccac
tacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccg
ccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagctta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtagg
atcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagatgtccagattagataaaagtaaagtg
attaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtg
tagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagatag
gcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgt
gctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaa
atcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttac
tttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccg
ccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatca
tatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaata
acactgatagtgctagtgtagatcac