BBa_K877000 1 miniA Mini CipA coding region 2012-09-28T11:00:00Z 2015-05-08T01:13:39Z This part was isolated from the cipA scaffoldin from Clostridium thermocellum. Two sets of primers were used - one to isolate the type 2 dockerin region (MiniA2) and another to isolate three type-one cohesin regions and a signaling peptide for export of the protein out of the cell (MiniA1). Therefore, this part does not have the cellulose binding region of C. thermocellum's cellulosome or 6 of the type one cohesin regions. This decreased the nucleotide sequence from 7kb to ~1kb. This part codes for a portion of the scaffoldin protein (cipA) from Clostridium thermocellum cellulosome. Two regions of cipA were ligated together to form this part: three type one cohesin regions and a type two dockerin region. A signaling peptide for export of the protein out of the cell is also coded in this part. This part is unfinished; it contains 6 pst1 sites within its coding sequence. false false _1139_ 0 10647 9 It's complicated true The size of the original genomic sequence from Clostridium thermocellum was reduced from 7kb to ~1kb. false Alie Abele, David Pohlman BBa_K877000_sequence 1 atgagaaaagtcatcagtatgctcttagttgtggctatgctgacgacgatttttgcggcgatgataccgcagacagtatcggcggccacaatgacagtcgagatcggcaaagttacagcagccgttggatcaaaagtagaaatacctataaccctgaaaggagtgccatccaaaggaatggccaattgcgacttcgtattgggttatgatccaaatgtgctggaagtaacagaagtaaaaccaggaagcataataaaagatccggatcctagcaagagctttgatagcgcaatatatccggatcgaaagatgattgtatttctgtttgcagaagacagtggaagaggaacgtatgcaataactcaggatggagtatttgcaacaattgtagccactgtcaaatcagctgcagcggcaccgattactttgcttgaagtaggtgcatttgcggacaacgatttagtagaaataagcacaacttttgtcgcgggcggagtaaatcttggtagttccgtaccgacaacacagccaaatgttccgtcagacggtgtggtagtagaaattggcaaagttacgggatctgttggaactacagttgaaatacctgtatatttcagaggagttccatccaaaggaatagcaaactgcgactttgtgttcagatatgatccgaatgtattggaaattatagggatagatcccggagacataatagttgacccgaatcctaccaagagctttgatactgcaatatatcctgacagaaagataatagtattcctgtttgcggaagacagcggaacaggagcgtatgcaataactaaagacggagtatttgcaaaaataagagcaactgtaaaatcaagtgctccgggctatattactttcgacgaagtaggtggatttgcagataatgacctggtagaacagaaggtatcatttatagacggtggtgttaacgttggctacaacatcgccgacaacaacaccgccagagccgacgataactccgaacaagttgacacttaagataggcagagcagaaggaagacctggagacacggtggaaataccggttaacttgtatggagtacctcaaaaaggaatagcaagcggtgacttcgtagtaagctatgacccgaatgtacttgagataatagagatagaaccgggagaattgatagttgacccgaatcctaccaagagctttgatactgctgcagtatatcctgacagaaagatgatagtattcctgtttgcggaagacagcggaacaggagcgtatgcaataactgaagatggagtatttgctacgatagtagcgaaagtaaaagaaggagcacctgaaggattcagtgcaatagaaatttctgagtttggtgcatttgcagataatgatctggtagaagtggaaactgaccttatcaatggtggagtacttgtaactaataaacctgtaatagaaggatataaagtatccggatacattttgccagacttctccttcgacgctactgttgcaccacttgtaaaggccggattcaaagttgaaatagtaggaacagaattgtatgcagtaacagatgcaaacggatactttgaaataaccggagtacctgcaaatgcaagcggatatacattgaagatttcaagagcaacttacttggacagagtaattgcaaatgttgtagtaacgggagatacttcagtttcaacttcacaggctccaataatgatgtgggtaggagacatagtgaaagacaattctatcaacctgttggacgttgcagaagttatccgttgcttcaacgctactaaaggaagcgcaaactacgtagaagaacttgacattaatagaaacggcgcaattaacatgcaagacataatgattgttcataagcactttggagctacatcaagtgattacgacgcacagtaaatattaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z