BBa_K877001
1
BBa_K877001
LPP-OmpA and CtCoh2 Combination
2012-09-30T11:00:00Z
2015-05-08T01:13:39Z
The type 2 cohesin domain of this sequence comes from "Clostridium thermocellum" genome sequence.
This part is a combination of the transmembrane anchoring protein, LPP-OmpA, from the 2008 Warsaw submission and the type 2 "Clostridium thermocellum" cohesin module.
false
false
_1139_
0
10647
9
In stock
false
The unstructuralized amino acid linker of LPP-OmpA in combination with a similar structure naturally occurring in the type 2 cohesin domain allowed the two domains to be combined without compromising functionality. The type cohesin amino acid linker was at the 5' end of the sequence while the LPP-OmpA amino acid linker was at the 3' end, because of this the sac1 restriction site added to the cohesin domain was added to the 5' end of its sequence.
false
David Pohlman, Alie Abele
annotation2205282
1
ATG
range2205282
1
4
6
annotation2205284
1
SacI
range2205284
1
459
464
annotation2205281
1
OmpA
range2205281
1
4
438
annotation2205283
1
linker
range2205283
1
439
464
annotation2205280
1
NdeI
range2205280
1
1
7
annotation2205285
1
BBa_K103006
range2205285
1
1
464
BBa_K877001_sequence
1
catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z