LOCUS       BBa_K879917             2072 bp    DNA     linear       22-Dec-2024
DEFINITION  LacO ori
ACCESSION   BBa_K879917
VERSION     BBa_K879917.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    2053..2072
                     /label=BioBrick prefix
     stem_loop       22..95
                     /label=E coli his operon terminator
     primer_bind     159..178
                     /label=VR
     misc_feature    263..877
                     /label=pMB1 derived replication origin
     stem_loop       1044..1149
                     /label=T0 terminator
     CDS             1162..1821
                     /label=Chloramphenicol resistance
     primer_bind     1935..1954
                     /label=VF2
     misc_feature    277..826
                     /label=RNA II
     promoter        836..841
                     /label=-10
     misc_feature    842..858
                     /label=LacO operator
     promoter        859..864
                     /label=-35
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 attgttatcc gctcacaatc tcaatgcatt cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1081 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1141 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     1201 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1261 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1321 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1381 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1441 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1501 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1561 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     1621 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1681 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1741 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1801 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1861 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     1921 gatcaactcg agtgccactt gacgtctaag aaaccattat tatcatgaca ttaacctata
     1981 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2041 atgatttctg gaattcgcgg ccgcttctag ag
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