LOCUS       BBa_K879918             2074 bp    DNA     linear       22-Nov-2024
DEFINITION  TetO ori
ACCESSION   BBa_K879918
VERSION     BBa_K879918.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     stem_loop       22..95
                     /label=E coli his operon terminator
     primer_bind     159..178
                     /label=VR
     misc_feature    263..877
                     /label=pMB1 derived replication origin
     misc_feature    277..826
                     /label=RNA II
     promoter        836..841
                     /label=-10
     misc_feature    842..858
                     /label=TetO operator
     promoter        859..864
                     /label=-35
     stem_loop       1044..1149
                     /label=T0 terminator
     CDS             1162..1821
                     /label=Chloramphenicol resistance
     primer_bind     1935..1954
                     /label=VF2
     misc_feature    2053..2072
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagagt
      841 atctctatca ctgataggga tgtcaatgca ttcctttgat cttttctacg gggtctgacg
      901 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
      961 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1021 aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac
     1081 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     1141 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     1201 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     1261 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     1321 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     1381 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     1441 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     1501 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     1561 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     1621 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     1681 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     1741 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     1801 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     1861 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     1921 ccgatcaact cgagtgccac ttgacgtcta agaaaccatt attatcatga cattaaccta
     1981 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     2041 ggatgatttc tggaattcgc ggccgcttct agag
//