BBa_K880001 1 BBa_K880001 Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K 2012-09-28T11:00:00Z 2015-05-08T01:13:40Z Created from a smattering of DNA junk and HbiF/FimE recognition sequences -IRR K137008 + Sequence with AgeI cut-site K165006 + IRL K137010(MI262) -Asymmetrically digestible reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases. K880001 is a utility for characterizing the functionality of fim based recombinases such as FimB, FimE, and HbiF and consists of K137008 and K137010 flanking a ???junk??? DNA sequence K165006 containing an off-center AgeI restriction site proximal to the BioBrick prefix; inversion of the fragment will cause the site to move towards the suffix. Digestion of the unflipped reporter with EcoRI and AgeI will yield a ~70bp fragment when in a FimE-flippable orientation and a ~220bp fragment when in an HbiF flippable orientation. Alternatively, digestion with AgeI and PstI will yield fragments of roughly opposite orientation. The reporter in the registry sequence is FimE flippable. false false _1142_ 0 9403 9 It's complicated false N/A false Josh Atkinson, Mike Ferguson, and Ben Parker component2204469 1 BBa_K137010 component2204465 1 BBa_K137008 component2204466 1 BBa_K165006 annotation2204469 1 BBa_K137010 range2204469 1 304 338 annotation2204465 1 BBa_K137008 range2204465 1 1 35 annotation2204466 1 BBa_K165006 range2204466 1 44 295 BBa_K165006 1 BBa_K165006 Zif268-HIV DNA-binding domain 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - - false false _267_ 0 2512 58 It's complicated true - false John Szymanski BBa_K137010 1 fimE IRL fimE IRL 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z pFIP plasmid fimE inverted repeat left recombination site false false _187_ 0 3112 9 It's complicated false none false Allen Lin annotation1963844 1 IRL out range1963844 1 19 35 annotation1963843 1 IRL in range1963843 1 1 17 BBa_K137008 1 IRR fimE IRR 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z pFIP plasmid fimE inverted repeat right recombination site false false _187_ 0 3112 9 It's complicated false none false Allen Lin annotation1963842 1 IRR in range1963842 1 19 35 annotation1963841 1 IRR out range1963841 1 1 17 BBa_K137010_sequence 1 tctatgagtcaaaatggccccaattgtcttgtatt BBa_K165006_sequence 1 cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaa BBa_K137008_sequence 1 aagatgaaacatttggggccaaactgtccatatta BBa_K880001_sequence 1 aagatgaaacatttggggccaaactgtccatattatactagagcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaatactagagtctatgagtcaaaatggccccaattgtcttgtatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z