BBa_K137010
1
fimE IRL
fimE IRL
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
pFIP plasmid
fimE inverted repeat left recombination site
false
false
_187_
0
3112
9
It's complicated
false
none
false
Allen Lin
annotation1963844
1
IRL out
range1963844
1
19
35
annotation1963843
1
IRL in
range1963843
1
1
17
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K137008
1
IRR
fimE IRR
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
pFIP plasmid
fimE inverted repeat right recombination site
false
false
_187_
0
3112
9
It's complicated
false
none
false
Allen Lin
annotation1963842
1
IRR in
range1963842
1
19
35
annotation1963841
1
IRR out
range1963841
1
1
17
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_I13504
1
BBa_I13504
Screening plasmid intermediate
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
Built by Josh as an intermediate in screening plasmid construction.
false
true
_11_
0
253
6
In stock
false
true
jkm
component1505067
1
BBa_B0034
component1505074
1
BBa_B0010
component1505084
1
BBa_B0012
component1505069
1
BBa_E0040
annotation1505074
1
BBa_B0010
range1505074
1
747
826
annotation1505067
1
BBa_B0034
range1505067
1
1
12
annotation1505069
1
BBa_E0040
range1505069
1
19
738
annotation1505084
1
BBa_B0012
range1505084
1
835
875
BBa_K880002
1
BBa_K880002
GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.
2012-09-28T11:00:00Z
2015-05-08T01:13:40Z
Enter source
Released HQ 2013
-IRR K137008 + inverted tetR promoter K137047 + IRL K137010 + GFP I13504 (MI275)
-GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases. Same components as K137058 with the inverted repeats reversed such that the external half sites are external to the flip region (IRR - ???flip region??? - IRL)
GFP fluorescent reporter capable of assaying the functionality of fim regulatory recombinases; based on K137058.
K137058???s invertible repeat sequences responsible for recombinase binding are ordered incorrectly; the regions are not identical, and have ???left??? and ???right??? components. K137008 (invertible repeat-right) and K137010 (invertible repeat-left) are mislabeled and belong in the order ???K137008-region to invert-K137010??? in accordance with the wild-type fim system.
Repeat sequence in this part exist in the correct (wild-type) orientation.
false
false
_1142_
0
9403
9
In stock
false
Enter design considerations
false
Josh Atkinson, Mike Ferguson, and Ben Parker
component2204548
1
BBa_K137010
component2204543
1
BBa_K137008
component2204545
1
BBa_K137047
component2204558
1
BBa_I13504
annotation2204545
1
BBa_K137047
range2204545
1
44
293
annotation2204558
1
BBa_I13504
range2204558
1
345
1219
annotation2204543
1
BBa_K137008
range2204543
1
1
35
annotation2204548
1
BBa_K137010
range2204548
1
302
336
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K137047
1
BBa_K137047
250 bp inverted tetR promoter
2008-07-20T11:00:00Z
2015-05-08T01:10:09Z
Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.
false
false
_187_
0
3112
9
It's complicated
false
This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
false
Allen Lin
annotation1968020
1
tetR promoter
range1968020
1
1
54
BBa_K137010_sequence
1
tctatgagtcaaaatggccccaattgtcttgtatt
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K137008_sequence
1
aagatgaaacatttggggccaaactgtccatatta
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K880002_sequence
1
aagatgaaacatttggggccaaactgtccatattatactagaggtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttttactagagtctatgagtcaaaatggccccaattgtcttgtatttactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_I13504_sequence
1
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K137047_sequence
1
gtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z