BBa_K883160
1
BBa_K883160
CCMV_Negative coat protein
2012-09-18T11:00:00Z
2015-05-08T01:13:40Z
Modified from CCMV wild-type.
This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues.
The full protocol for this construct can be found on the Wageningen_UR 2012 Wiki.
false
false
_1146_
0
9618
9
It's complicated
false
In nature, wild-type CCMV Virus Particles have a positive interior to which RNA molecules attach. To be able to load the CCMV VLPs with positively charged molecules, e.g. metals, we have re-designed the N-Terminus of the core antigen subunits to contain 9 negatively charged amino acids instead of 9 positively charged amino acids.
false
Hugo de Vries
annotation2187438
1
CCMV with negative N-terminus
range2187438
1
1
578
BBa_K883160_sequence
1
atgggtacagtcggaacaggggaacttacggaagctcaagaagaagctgctgctgaagaaaacgaagaaaacacggaagtggtccaacctgttattgtagaacccatcgcttcaggccaaggcaaggctattaaagcatggaccggttacagcgtatcgaagtggaccgcctcttgtgcggctgccgaagctaaagtaacctcggctataactatctctctccctaatgagctatcgtccgaaaggaacaagcagctcaaggtaggtagagttttattatggcttgggttgcttcccagtgttagtggcacagtgaaatcctgtgttacagagacgcagactactgctgctgcctcctttcaggtggcattagctgtggccgacaactcgaaagatgttgtcgctgctatgtaccccgaggcgtttaagggtataacccttgaacaactcaccgcggatttaacgatctacttgtacagcagtgcggctctcactgagggcgacgtcatcgtgcatttggaggttgagcatgtcagacctacgtttgacgactctttcactccggtgtattagtgaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z