BBa_K883161 1 BBa_K883161 CCMV_Negative core antigen with IPTG induced promotor 2012-09-23T11:00:00Z 2015-05-08T01:13:40Z Cow Chlorotic Mottle Virus (CCMV) This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues. The promotor and RBS that have been added to the gene allow induced expression of the subunits which increases the yield. The full protocol for this construct can be found on the Wageningen_UR 2012 Wiki. false false _1146_ 0 9618 9 It's complicated false 6 Arginine and 3 Lysine residues at the N-Terminus were replaced with Glutamic acid residues. false Hugo de Vries annotation2195454 1 R0010 range2195454 1 3 202 annotation2195461 1 CCMV with negative N-terminus range2195461 1 229 806 annotation2195450 1 B0034 range2195450 1 211 222 BBa_K883161_sequence 1 agcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggtacagtcggaacaggggaacttacggaagctcaagaagaagctgctgctgaagaaaacgaagaaaacacggaagtggtccaacctgttattgtagaacccatcgcttcaggccaaggcaaggctattaaagcatggaccggttacagcgtatcgaagtggaccgcctcttgtgcggctgccgaagctaaagtaacctcggctataactatctctctccctaatgagctatcgtccgaaaggaacaagcagctcaaggtaggtagagttttattatggcttgggttgcttcccagtgttagtggcacagtgaaatcctgtgttacagagacgcagactactgctgctgcctcctttcaggtggcattagctgtggccgacaactcgaaagatgttgtcgctgctatgtaccccgaggcgtttaagggtataacccttgaacaactcaccgcggatttaacgatctacttgtacagcagtgcggctctcactgagggcgacgtcatcgtgcatttggaggttgagcatgtcagacctacgtttgacgactctttcactccggtgtattagtgaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z