BBa_K883201 1 BBa_K883201 Hepatitits B core antigen with IPTG induced promotor 2012-09-16T11:00:00Z 2015-05-08T01:13:40Z The parts has been amplified from a plasmid used for the production of HepBcAg in plants: pEAQ-HT-wtHBcAg-EL This construct produces Hepatitis B core antigen subunits. These subunits can be used to make HepBcAg Virus-Like Particles. The promotor and RBS that have been added to the gene allow induced expression of the subunits which increases the yield. The HepBcAg subunits can be easily modified in an external loop. false false _1146_ 0 12653 9 It's complicated true The sequence was checked for illegal restriction sites. There were none present. The primers used to amplify the gene contained the prefix and suffix used in the standard 10 assembly. false Kees van der Ark annotation2184131 1 B0034 range2184131 1 209 220 annotation2184132 1 Hepatitis B core Antigen range2184132 1 221 823 annotation2184122 1 R0010 range2184122 1 1 200 BBa_K883201_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggatatcgacccatataaagaatttggcgcgacggttgagctgctgagctttctgccaagcgatttctttccgagcgtccgcgacctgctggataccgccagcgcactgtatcgtgaagccctggagagcccggaacattgcagcccgcatcatacggccctgcgtcaggcaatcctgtgctggggcgaactgatgaccctggcaacctgggtcggcaataatctggtcgacgctggtggaggtcctaggggtgggtctggtattaatgacccagcaagccgtgatctggttgttaattacgtgaacaccaacatgggcctgaagatccgccaactgctgtggtttcatatcagctgtctgacgtttggccgcgagacggtgctggaatacctggttagctttggcgtttggattcgtacgccaccggcctaccgcccaccaaacgcaccgattctgagcacgctgccggaaacgacggttgttcgtcgccgtgatcgcggccgtagcccgcgccgccgtacgccgagcccacgtcgtcgccgcagccagagcccgcgccgccgtcgcagccagagccgtgaaagccaatgttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z