LOCUS       BBa_K883703             1021 bp    DNA     linear       22-Dec-2024
DEFINITION  IPTG induced promoter + GFP with a His-tag fused to a coiled coil
ACCESSION   BBa_K883703
VERSION     BBa_K883703.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=IPTG induced promoter
     RBS             208..220
                     /label=RBS
     CDS             221..1000
                     /label=GFP
     misc_feature    1001..1021
                     /label=His tag
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa atggaaattg cggcgctgga
      241 aaaagagatt gcagcgcttg agaaagagat cgccgccctg gagaagatgc gtaaaggaga
      301 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      361 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      421 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      481 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      541 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      601 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      661 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      721 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      781 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      841 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      901 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      961 agctgctggg attacacatg gcatggatga actatacaaa catcaccatc accatcacta
     1021 g
//