LOCUS       BBa_K883705             1127 bp    DNA     linear       22-Nov-2024
DEFINITION  IPTG induced promoter + GFP with a His-tag fused to a coiled coil
ACCESSION   BBa_K883705
VERSION     BBa_K883705.1  
FEATURES             Location/Qualifiers
     promoter        1..220
                     /label=IPTG induced promoter
     CDS             253..343
                     /label=coiled coil
     CDS             344..1111
                     /label=GFP
     misc_feature    1112..1127
                     /label=His tag
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagaaag atgaattcgc
      241 ggccgcttat agagatggaa attgcggcgc tggaaaaaga gattgcagcg cttgagaaag
      301 agatcgccgc cctggagaag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      361 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      421 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      481 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      541 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtgt
      601 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
      661 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      721 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
      781 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
      841 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
      901 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      961 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1021 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     1081 gattacacat ggcatggatg aactatacaa acatcaccat caccatc
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