BBa_K887000
type: ComponentDefinition
persistent identity: BBa K887000
display id: BBa_K887000
version: 1
was generated by: iGEM to SBOL conversion
description: Plac+B0034+zif268+alsS+B0034+PBSII+ilvC+B0034+HIVC+ilvD+37℃ induced RBS+tetR+double terminator
created: 2012-08-18T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: 123
owned by: james
owned by: myers
mutable description: 123
discontinued: false
dominant: false
experience: experience/Works
group u list: _1150_
m user id: 0
owner id: 10239
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: 123
star: false
creator: LIN I
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_K115002
component: BBa_K539712
component: BBa_R0010
component: BBa_K323105
component: BBa_J61048
component: BBa_C0040
component: BBa_K539713
component: BBa_K539711
component: BBa_K323107
component: BBa_K165006
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K323105
sequence annotation: BBa_K539713
sequence annotation: BBa_K323107
sequence annotation: BBa_K539711
sequence annotation: BBa_K165006
sequence annotation: BBa_K539712
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K887000_sequence/1

BBa_B0034
type: Component
persistent identity: component2301213
display id: component2301213
version: 1
top level: BBa_K887000
definition: BBa_B0034
access: public

BBa_K115002
type: Component
persistent identity: component2301221
display id: component2301221
version: 1
top level: BBa_K887000
definition: BBa_K115002
access: public

BBa_K539712
type: Component
persistent identity: component2301216
display id: component2301216
version: 1
top level: BBa_K887000
definition: BBa_K539712
access: public

BBa_R0010
type: Component
persistent identity: component2301195
display id: component2301195
version: 1
top level: BBa_K887000
definition: LacI
access: public

BBa_K323105
type: Component
persistent identity: component2301204
display id: component2301204
version: 1
top level: BBa_K887000
definition: BBa_K323105
access: public

BBa_B0034
type: Component
persistent identity: component2301203
display id: component2301203
version: 1
top level: BBa_K887000
definition: BBa_B0034
access: public

BBa_J61048
type: Component
persistent identity: component2301226
display id: component2301226
version: 1
top level: BBa_K887000
definition: BBa_J61048
access: public

BBa_C0040
type: Component
persistent identity: component2301225
display id: component2301225
version: 1
top level: BBa_K887000
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component2301208
display id: component2301208
version: 1
top level: BBa_K887000
definition: BBa_B0034
access: public

BBa_K539713
type: Component
persistent identity: component2301206
display id: component2301206
version: 1
top level: BBa_K887000
definition: BBa_K539713
access: public

BBa_K539711
type: Component
persistent identity: component2301211
display id: component2301211
version: 1
top level: BBa_K887000
definition: BBa_K539711
access: public

BBa_K323107
type: Component
persistent identity: component2301209
display id: component2301209
version: 1
top level: BBa_K887000
definition: BBa_K323107
access: public

BBa_K165006
type: Component
persistent identity: component2301214
display id: component2301214
version: 1
top level: BBa_K887000
definition: BBa_K165006
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2301195
display id: annotation2301195
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301195/range2301195/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K887000/annotation2301195/range2301195/1
type: Range
persistent identity: range2301195
display id: range2301195
version: 1
top level: BBa_K887000
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2301203
display id: annotation2301203
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301203/range2301203/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887000/annotation2301203/range2301203/1
type: Range
persistent identity: range2301203
display id: range2301203
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K323105
type: SequenceAnnotation
persistent identity: annotation2301204
display id: annotation2301204
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301204/range2301204/1
component: BBa_K323105

https://synbiohub.org/public/igem/BBa_K887000/annotation2301204/range2301204/1
type: Range
persistent identity: range2301204
display id: range2301204
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 229
end: 522
orientation: inline

BBa_K539713
type: SequenceAnnotation
persistent identity: annotation2301206
display id: annotation2301206
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301206/range2301206/1
component: BBa_K539713

https://synbiohub.org/public/igem/BBa_K887000/annotation2301206/range2301206/1
type: Range
persistent identity: range2301206
display id: range2301206
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 529
end: 2244
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2301208
display id: annotation2301208
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301208/range2301208/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887000/annotation2301208/range2301208/1
type: Range
persistent identity: range2301208
display id: range2301208
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 2253
end: 2264
orientation: inline

BBa_K323107
type: SequenceAnnotation
persistent identity: annotation2301209
display id: annotation2301209
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301209/range2301209/1
component: BBa_K323107

https://synbiohub.org/public/igem/BBa_K887000/annotation2301209/range2301209/1
type: Range
persistent identity: range2301209
display id: range2301209
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 2273
end: 2560
orientation: inline

BBa_K539711
type: SequenceAnnotation
persistent identity: annotation2301211
display id: annotation2301211
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301211/range2301211/1
component: BBa_K539711

https://synbiohub.org/public/igem/BBa_K887000/annotation2301211/range2301211/1
type: Range
persistent identity: range2301211
display id: range2301211
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 2567
end: 4042
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2301213
display id: annotation2301213
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301213/range2301213/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887000/annotation2301213/range2301213/1
type: Range
persistent identity: range2301213
display id: range2301213
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 4051
end: 4062
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation2301214
display id: annotation2301214
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301214/range2301214/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K887000/annotation2301214/range2301214/1
type: Range
persistent identity: range2301214
display id: range2301214
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 4071
end: 4322
orientation: inline

BBa_K539712
type: SequenceAnnotation
persistent identity: annotation2301216
display id: annotation2301216
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301216/range2301216/1
component: BBa_K539712

https://synbiohub.org/public/igem/BBa_K887000/annotation2301216/range2301216/1
type: Range
persistent identity: range2301216
display id: range2301216
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 4329
end: 6179
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2301221
display id: annotation2301221
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301221/range2301221/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K887000/annotation2301221/range2301221/1
type: Range
persistent identity: range2301221
display id: range2301221
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 6188
end: 6239
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2301225
display id: annotation2301225
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301225/range2301225/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K887000/annotation2301225/range2301225/1
type: Range
persistent identity: range2301225
display id: range2301225
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 6246
end: 6930
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2301226
display id: annotation2301226
version: 1
top level: BBa_K887000
location: https://synbiohub.org/public/igem/BBa_K887000/annotation2301226/range2301226/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K887000/annotation2301226/range2301226/1
type: Range
persistent identity: range2301226
display id: range2301226
version: 1
top level: BBa_K887000
direction: direction/reverse
start: 6939
end: 7051
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K323107
type: ComponentDefinition
persistent identity: BBa K323107
display id: BBa_K323107
version: 1
was generated by: iGEM to SBOL conversion
description: PBSII zinc finger + linker
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Tina Lebar
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K323107_sequence/1

BBa_K323105
type: ComponentDefinition
persistent identity: BBa K323105
display id: BBa_K323105
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268 zinc finger + linker
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Tina Lebar
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K323105_sequence/1

BBa_K539711
type: ComponentDefinition
persistent identity: BBa K539711
display id: BBa_K539711
version: 1
was generated by: iGEM to SBOL conversion
description: ilvC (ketol-acid reductoisomerase NAD(P)-binding)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding region of ilvC from Escherichia coli str. K-12 substr(MG1655). It encodes ketol-acid reductoisomerase NAD(P)-binding which is part of the isobutanol biosynthesis pathway.
discontinued: false
dominant: false
experience: experience/None
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding gene, we have to delete those RE site in kivd. The RE site in the part has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ilvC coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K539711_sequence/1

ilvC coding sequence
type: SequenceAnnotation
persistent identity: annotation2136206
display id: annotation2136206
version: 1
top level: BBa_K539711
location: https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
type: Range
persistent identity: range2136206
display id: range2136206
version: 1
top level: BBa_K539711
direction: direction/forward
start: 1
end: 1476
orientation: inline

BBa_K539713
type: ComponentDefinition
persistent identity: BBa K539713
display id: BBa_K539713
version: 1
was generated by: iGEM to SBOL conversion
description: AlsS (alpha-acetolactate synthase protein)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Bacillus subtilis.
owned by: james
owned by: myers
mutable description: This is the coding region of AlsS from Bacillus subtilis. It encodes alpha-acetolactate synthase protein which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AlsS
sequence: https://synbiohub.org/public/igem/BBa_K539713_sequence/1

AlsS
type: SequenceAnnotation
persistent identity: annotation2136210
display id: annotation2136210
version: 1
top level: BBa_K539713
location: https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
type: Range
persistent identity: range2136210
display id: range2136210
version: 1
top level: BBa_K539713
direction: direction/forward
start: 1
end: 1716
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_K115002
type: ComponentDefinition
persistent identity: BBa K115002
display id: BBa_K115002
version: 1
was generated by: iGEM to SBOL conversion
description: RNA thermometer (FourU)
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
mutable provenance: Salmonella Enterica Tyhpy (CP000886.1)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Part of the sequence is altered because of the scar...
star: true
creator: Bastiaan van den Berg
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: stem_loop
sequence annotation: scar adaptation
sequence annotation: predicted stem-loop extending to one position before start codon
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K115002_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1966930
display id: annotation1966930
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
type: Range
persistent identity: range1966930
display id: range1966930
version: 1
top level: BBa_K115002
direction: direction/forward
start: 1
end: 18
orientation: inline

scar adaptation
type: SequenceAnnotation
persistent identity: annotation1966899
display id: annotation1966899
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
type: Range
persistent identity: range1966899
display id: range1966899
version: 1
top level: BBa_K115002
direction: direction/forward
start: 23
end: 28
orientation: inline

predicted stem-loop extending to one position before start codon
type: SequenceAnnotation
persistent identity: annotation1966931
display id: annotation1966931
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
type: Range
persistent identity: range1966931
display id: range1966931
version: 1
top level: BBa_K115002
direction: direction/forward
start: 24
end: 52
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1966897
display id: annotation1966897
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
type: Range
persistent identity: range1966897
display id: range1966897
version: 1
top level: BBa_K115002
direction: direction/forward
start: 47
end: 52
orientation: inline

BBa_K165006
type: ComponentDefinition
persistent identity: BBa K165006
display id: BBa_K165006
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV DNA-binding domain
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K165006_sequence/1

BBa_K539712
type: ComponentDefinition
persistent identity: BBa K539712
display id: BBa_K539712
version: 1
was generated by: iGEM to SBOL conversion
description: ilvD (dihydroxyacid dehydratase)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding sequence of ilvD form Escherichia coli str. K-12 substr. MG1655. It encodes dihydroxyacid dehydratase which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There is one Pst1 in original sequence of ilvD. However, the RE site in ilvD has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ilvD
sequence: https://synbiohub.org/public/igem/BBa_K539712_sequence/1

ilvD
type: SequenceAnnotation
persistent identity: annotation2136209
display id: annotation2136209
version: 1
top level: BBa_K539712
location: https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
type: Range
persistent identity: range2136209
display id: range2136209
version: 1
top level: BBa_K539712
direction: direction/forward
start: 1
end: 1851
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: iGEM to SBOL conversion
description: [rnpB-T1] Terminator
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

https://synbiohub.org/public/igem/BBa_K887000_sequence/1
type: Sequence
persistent identity: BBa K887000 sequence
display id: BBa_K887000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcaggtggtagttactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K323107_sequence/1
type: Sequence
persistent identity: BBa K323107 sequence
display id: BBa_K323107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgggtgaaaaaccgtatgcatgtcctgaatgtggtaaaagctttagccagcgtgcaaatctgcgtgcacatcagcgtacccatacaggtgaaaagccttataaatgcccagaatgcggcaaaagcttttcacgtagcgatcatctgaccacccatcagcgcacacatactggcgagaaaccttacaaatgtccagagtgtggtaaatcatttagccgtagtgatgttctggttcgtcatcagagaacccacacgggtggtggtagcggtggtggttcaggtggtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K323105_sequence/1
type: Sequence
persistent identity: BBa K323105 sequence
display id: BBa_K323105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcaggtggtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539711_sequence/1
type: Sequence
persistent identity: BBa K539711 sequence
display id: BBa_K539711_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539713_sequence/1
type: Sequence
persistent identity: BBa K539713 sequence
display id: BBa_K539713_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115002_sequence/1
type: Sequence
persistent identity: BBa K115002 sequence
display id: BBa_K115002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165006_sequence/1
type: Sequence
persistent identity: BBa K165006 sequence
display id: BBa_K165006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539712_sequence/1
type: Sequence
persistent identity: BBa K539712 sequence
display id: BBa_K539712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61048_sequence/1
type: Sequence
persistent identity: BBa J61048 sequence
display id: BBa_J61048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z