LOCUS BBa_K887002 8066 bp DNA linear 25-Nov-2024 DEFINITION Plac+B0034+alsS+B0034+ilvC+B0034+ilvD+37℃RBS+tetR+double terminator+Ptet+B0032+kivD+B0015 ACCESSION BBa_K887002 VERSION BBa_K887002.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1942 /label=AlsS CDS 227..1942 /label=BBa_K539713 misc_feature 1955..1958 /label=conserved RBS 1951..1962 /label=BBa_B0034 CDS 1969..3444 /label=ilvC coding sequence CDS 1969..3444 /label=BBa_K539711 misc_feature 3457..3460 /label=conserved RBS 3453..3464 /label=BBa_B0034 CDS 3471..5321 /label=ilvD CDS 3471..5321 /label=BBa_K539712 RBS 5376..5381 /label=rbs misc_feature 5352..5357 /label=scar adaptation stem_loop 5330..5347 /label=stem_loop stem_loop 5353..5381 /label=predicted stem-loop extending to one position before start codon misc_feature 5330..5381 /label=BBa_K115002 CDS 5391..6007 /label=tetR misc_feature 6008..6041 /label=SsrA misc_feature 6048..6072 /label=Help:Barcodes CDS 5388..6072 /label=BBa_C0040 terminator 6081..6193 /label=BBa_J61048 misc_feature 6202..6220 /label=TetR 1 misc_feature 6202..6255 /label=BBa_R0040 promoter 6221..6226 /label=-35 misc_feature 6227..6245 /label=TetR 2 promoter 6244..6249 /label=-10 promoter 6202..6255 /label=BBa_R0040 RBS 6264..6276 /label=RBS-3\Weak RBS 6270..6273 /label=RBS misc_feature 6264..6276 /label=BBa_B0032 RBS 6264..6276 /label=BBa_B0032 CDS 6283..7929 /label=kivd coding sequence CDS 6283..7929 /label=BBa_K539714 stem_loop 7949..7992 /label=stem_loop misc_feature 7938..8017 /label=BBa_B0010 terminator 7938..8017 /label=BBa_B0010 stem_loop 8033..8052 /label=T7 TE misc_feature 8059..8059 /label=stop polyA_site 8053..8066 /label=polya misc_feature 8026..8066 /label=BBa_B0012 terminator 8026..8066 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt tgacaaaagc 241 aacaaaagaa caaaaatccc ttgtgaaaaa cagaggggcg gagcttgttg ttgattgctt 301 agtggagcaa ggtgtcacac atgtatttgg cattccaggt gcaaaaattg atgcggtatt 361 tgacgcttta caagataaag gacctgaaat tatcgttgcc cggcacgaac aaaacgcagc 421 attcatggcc caagcagtcg gccgtttaac tggaaaaccg ggagtcgtgt tagtcacatc 481 aggaccgggt gcctctaact tggcaacagg cctgctgaca gcgaacactg aaggagaccc 541 tgtcgttgcg cttgctggaa acgtgatccg tgcagatcgt ttaaaacgga cacatcaatc 601 tttggataat gcggcgctat tccagccgat tacaaaatac agtgtagaag ttcaagatgt 661 aaaaaatata ccggaagctg ttacaaatgc atttaggata gcgtcagcag ggcaggctgg 721 ggccgctttt gtgagctttc cgcaagatgt tgtgaatgaa gtcacaaata cgaaaaacgt 781 gcgtgctgtt gcagcgccaa aactcggtcc agcagcagat gatgcaatca gtgcggccat 841 agcaaaaatc caaacagcaa aacttcctgt cgttttggtc ggcatgaaag gcggaagacc 901 ggaagcaatt aaggcggttc gcaagctttt gaaaaaggtt cagcttccat ttgttgaaac 961 atatcaagct gccggtaccc tttccagaga tttagaggat caatattttg gccgtatcgg 1021 tttgttccgc aaccagcctg gcgatttact gctagagcag gcagatgttg ttctgacgat 1081 cggctatgac ccgattgaat atgatccgaa attctggaat atcaatggag accggacaat 1141 tatccattta gacgagatta tcgctgacat tgatcatgct taccagcctg atcttgaatt 1201 gatcggtgac attccgtcca cgatcaatca tatcgaacac gatgctgtga aagtggaatt 1261 tgcagagcgt gagcagaaaa tcctttctga tttaaaacaa tatatgcatg aaggtgagca 1321 ggtgccagca gattggaaat cagacagagc gcaccctctt gaaatcgtta aagagttgcg 1381 taatgcagtc gatgatcatg ttacagtaac ttgcgatatc ggttcgcacg ccatttggat 1441 gtcacgttat ttccgcagct acgagccgtt aacattaatg atcagtaacg gtatgcaaac 1501 actcggcgtt gcgcttcctt gggcaatcgg cgcttcattg gtgaaaccgg gagaaaaagt 1561 ggtttctgtc tctggtgacg gcggtttctt attctcagca atggaattag agacagcagt 1621 tcgactaaaa gcaccaattg tacacattgt atggaacgac agcacatatg acatggttgc 1681 attccagcaa ttgaaaaaat ataaccgtac atctgcggtc gatttcggaa atatcgatat 1741 cgtgaaatat gcggaaagct tcggagcaac tggcttgcgc gtagaatcac cagaccagct 1801 ggcagatgtt ctgcgtcaag gcatgaacgc tgaaggtcct gtcatcatcg atgtcccggt 1861 tgactacagt gataacatta atttagcaag tgacaagctt ccgaaagaat ttggggaact 1921 catgaaaacg aaagctctct agtactagag aaagaggaga aatactagat ggctaactac 1981 ttcaatacac tgaatctgcg ccagcagctg gcacagctgg gcaaatgtcg ctttatgggc 2041 cgcgatgaat ttgccgatgg cgcgagctac cttcagggta aaaaagtagt catcgtcggc 2101 tgtggcgcac agggtctgaa ccagggcctg aacatgcgtg attctggtct cgatatctcc 2161 tacgctctgc gtaaagaagc gattgccgag aagcgcgcgt cctggcgtaa agcgaccgaa 2221 aatggtttta aagtgggtac ttacgaagaa ctgatcccac aggcggatct ggtgattaac 2281 ctgacgccgg acaagcagca ctctgatgta gtgcgcaccg tacagccact gatgaaagac 2341 ggcgcggcgc tgggctactc gcacggtttc aacatcgtcg aagtgggcga gcagatccgt 2401 aaagatatca ccgtagtgat ggttgcgccg aaatgcccag gcaccgaagt gcgtgaagag 2461 tacaaacgtg ggttcggcgt accgacgctg attgccgttc acccggaaaa cgatccgaaa 2521 ggcgaaggca tggcgattgc caaagcctgg gcggctgcaa ccggtggtca ccgtgcgggt 2581 gtgctggaat cgtccttcgt tgcggaagtg aaatctgatc tgatgggcga gcaaaccatc 2641 ctgtgcggta tgttgcaggc tggctctctg ctgtgcttcg acaagctggt ggaagaaggt 2701 accgatccag catacgcaga aaaactgatt cagttcggtt gggaaaccat caccgaagca 2761 ctgaaacagg gcggcatcac cctgatgatg gaccgtctct ctaacccggc gaaactgcgt 2821 gcttatgcgc tttctgaaca gctgaaagag atcatggcac ccctgttcca gaaacatatg 2881 gacgacatca tctccggcga attttcttcc ggtatgatgg cggactgggc caacgatgat 2941 aagaaactgc tgacctggcg tgaagagacc ggcaaaaccg cgtttgaaac cgcgccgcag 3001 tatgaaggca aaatcggcga gcaggagtac ttcgataaag gcgtactgat gattgcgatg 3061 gtgaaagcgg gcgttgaact ggcgttcgaa accatggtcg attccggcat cattgaagag 3121 tctgcatatt atgaatcact gcacgagctg ccgctgattg ccaacaccat cgcccgtaag 3181 cgtctgtacg aaatgaacgt ggttatctct gataccgctg agtacggtaa ctatctgttc 3241 tcttacgctt gtgtgccgtt gctgaaaccg tttatggcag agctgcaacc gggcgacctg 3301 ggtaaagcta ttccggaagg cgcggtagat aacgggcaac tgcgtgatgt gaacgaagcg 3361 attcgcagcc atgcgattga gcaggtaggt aagaaactgc gcggctatat gacagatatg 3421 aaacgtattg ctgttgcggg ttaatactag agaaagagga gaaatactag atgcctaagt 3481 accgttccgc caccaccact catggtcgta atatggcggg tgctcgtgcg ctgtggcgcg 3541 ccaccggaat gaccgacgcc gatttcggta agccgattat cgcggttgtg aactcgttca 3601 cccaatttgt accgggtcac gtccatctgc gcgatctcgg taaactggtc gccgaacaaa 3661 ttgaagcggc tggcggcgtt gccaaagagt tcaacaccat tgcggtggat gatgggattg 3721 ccatgggcca cggggggatg ctttattcac tgccatctcg cgaactgatc gctgattccg 3781 ttgagtatat ggtcaacgcc cactgcgccg acgccatggt ctgcatctct aactgcgaca 3841 aaatcacccc ggggatgctg atggcttccc tgcgcctgaa tattccggtg atctttgttt 3901 ccggcggccc gatggaggcc gggaaaacca aactttccga tcagatcatc aagctcgatc 3961 tggtcgatgc gatgatccag ggcgcagacc cgaaagtatc tgactcccag agcgatcagg 4021 ttgaacgttc cgcgtgtccg acctgcggtt cctgctccgg gatgtttacc gctaactcaa 4081 tgaactgcct gaccgaagcg ctgggcctgt cgcagccggg caacggctcg ctgctggcaa 4141 cccacgccga ccgtaagcag ctgttcctta atgctggtaa acgcattgtt gaattgacca 4201 aacgttatta cgagcaaaac gacgaaagtg cactgccgcg taatatcgcc agtaaggcgg 4261 cgtttgaaaa cgccatgacg ctggatatcg cgatgggtgg atcgactaac accgtacttc 4321 acctgctggc ggcggcgcag gaagcggaaa tcgacttcac catgagtgat atcgataagc 4381 tttcccgcaa ggttccacag ctgtgtaaag ttgcgccgag cacccagaaa taccatatgg 4441 aagatgttca ccgtgctggt ggtgttatcg gtattctcgg cgaactggat cgcgcggggt 4501 tactgaaccg tgatgtgaaa aacgtacttg gcctgacgtt gccgcaaacg ctggaacaat 4561 acgacgttat gctgacccag gatgacgcgg taaaaaatat gttccgcgca ggtccagcag 4621 gcattcgtac cacacaggca ttctcgcaag attgccgttg ggatacgctg gacgacgatc 4681 gcgccaatgg ctgtatccgc tcgctggaac acgcctacag caaagacggc ggcctggcgg 4741 tgctctacgg taactttgcg gaaaacggct gcatcgtgaa aacggcaggc gtcgatgaca 4801 gcatcctcaa attcaccggc ccggcgaaag tgtacgaaag ccaggacgat gcggtagaag 4861 cgattctcgg cggtaaagtt gtcgccggag atgtggtagt aattcgctat gaaggcccga 4921 aaggcggtcc ggggatgcag gaaatgctct acccaaccag cttcctgaaa tcaatgggtc 4981 tcggcaaagc ctgtgcgctg atcaccgacg gtcgtttctc tggtggcacc tctggtcttt 5041 ccatcggcca cgtctcaccg gaagcggcaa gcggcggcag cattggcctg attgaagatg 5101 gtgacctgat cgctatcgac atcccgaacc gtggcattca gttacaggta agcgatgccg 5161 aactggcggc gcgtcgtgaa gcgcaggacg ctcgaggtga caaagcctgg acgccgaaaa 5221 atcgtgaacg tcaggtctcc tttgccctgc gtgcttatgc cagcctggca accagcgccg 5281 acaaaggcgc ggtgcgcgat aaatcgaaac tggggggtta atactagagg gacaagcaat 5341 gcttgccttg aatagtaact tttgaatagt gattcaggag gtactagatg tccagattag 5401 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt 5461 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg 5521 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata 5581 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa 5641 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc 5701 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt 5761 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg 5821 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata 5881 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag 5941 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa 6001 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 6061 agtgtagatc actactagag ccggcttatc ggtcagtttc acctgattta cgtaaaaacc 6121 cgcttcggcg ggtttttgct tttggagggg cagaaagatg aatgactgtc cacgacgcta 6181 tacccaaaag aaatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat 6241 agagatactg agcactacta gagtcacaca ggaaagtact agatgtatac agtaggagat 6301 tacctattag accgattaca cgagttagga attgaagaaa tttttggagt ccctggagac 6361 tataacttac aatttttaga tcaaattatt tcccgcaagg atatgaaatg ggtcggaaat 6421 gctaatgaat taaatgcttc atatatggct gatggctatg ctcgtactaa aaaagctgcc 6481 gcatttctta caacctttgg agtaggtgaa ttgagtgcag ttaatggatt agcaggaagt 6541 tacgccgaaa atttaccagt agtagaaata gtgggatcac ctacatcaaa agttcaaaat 6601 gaaggaaaat ttgttcatca tacgctggct gacggtgatt ttaaacactt tatgaaaatg 6661 cacgaacctg ttacagcagc tcgaacttta ctgacagcag aaaatgcaac cgttgaaatt 6721 gaccgagtac tttctgcact attaaaagaa agaaaacctg tctatatcaa cttaccagtt 6781 gatgttgctg ctgcaaaagc agagaaaccc tcactccctt tgaaaaaaga aaactcaact 6841 tcaaatacaa gtgaccaaga gatcttgaac aaaattcaag aaagcttgaa aaatgccaaa 6901 aaaccaatcg tgattacagg acatgaaata attagttttg gcttagaaaa aacagtctct 6961 caatttattt caaagacaaa actacctatt acgacattaa actttggaaa aagttcagtt 7021 gatgaagctc tcccttcatt tttaggaatc tataatggta aactctcaga gcctaatctt 7081 aaagtattcg tggaatcagc cgacttcatc ctgatgcttg gagttaaact cacagactct 7141 tcaacaggag ccttcactca tcatttaaat gaaaataaaa tgatttcact gaatatagat 7201 gaaggaaaaa tatttaacga aagcatccaa aattttgatt ttgaatccct catctcctct 7261 ctcttagacc taagcgaaat agaatacaaa ggaaaatata tcgataaaaa gcaagaagac 7321 tttgttccat caaatgcgct tttatcacaa gaccgcctat ggcaagcagt tgaaaaccta 7381 actcaaagca atgaaacaat cgttgctgaa caagggacat cattctttgg cgcttcatca 7441 attttcttaa aaccaaagag tcattttatt ggtcaaccct tatggggatc aattggatat 7501 acattcccag cagcattagg aagccaaatt gcagataaag aaagcagaca ccttttattt 7561 attggtgatg gttcacttca acttacggtg caagaattag gattagcaat cagagaaaaa 7621 attaatccaa tttgctttat tatcaataat gatggttata cagtcgaaag agaaattcat 7681 ggaccaaatc aaagctacaa tgatattcca atgtggaatt actcaaaatt accagaatca 7741 tttggagcaa cagaagaacg agtagtctcg aaaatcgtta gaactgaaaa tgaatttgtg 7801 tctgtcatga aagaagctca agcagatcca aatagaatgt actggattga gttaattttg 7861 gcaaaagaag atgcaccaaa agtactgaaa aaaatgggca aactatttgc tgaacaaaat 7921 aaatcataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 7981 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 8041 ttcgggtggg cctttctgcg tttata //