##gff-version 3
##sequence-region BBa_K887002 1 8066
BBa_K887002	.	promoter	6202	6255	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K887002	.	engineered_region	6202	6255	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K887002	.	promoter	6221	6226	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K887002	.	promoter	6244	6249	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K887002	.	non_covalent_binding_site	6202	6220	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K887002	.	non_covalent_binding_site	6227	6245	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K887002	.	ribosome_entry_site	6264	6276	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K887002	.	engineered_region	6264	6276	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K887002	.	ribosome_entry_site	6270	6273	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K887002	.	ribosome_entry_site	6264	6276	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K887002	.	CDS	3471	5321	.	+	0	ID=BBa_K539712;Name=BBa_K539712
BBa_K887002	.	CDS	3471	5321	.	+	0	ID=annotation2136209;Name=ilvD;Parent=BBa_K539712
BBa_K887002	.	CDS	1969	3444	.	+	0	ID=BBa_K539711;Name=BBa_K539711
BBa_K887002	.	CDS	1969	3444	.	+	0	ID=annotation2136206;Name=ilvC coding sequence;Parent=BBa_K539711
BBa_K887002	.	ribosome_entry_site	1951	1962	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887002	.	conserved_region	1955	1958	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887002	.	terminator	7938	8017	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K887002	.	stem_loop	7949	7992	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K887002	.	engineered_region	7938	8017	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K887002	.	CDS	227	1942	.	+	0	ID=BBa_K539713;Name=BBa_K539713
BBa_K887002	.	CDS	227	1942	.	+	0	ID=annotation2136210;Name=AlsS;Parent=BBa_K539713
BBa_K887002	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887002	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887002	.	mature_transcript_region	5330	5381	.	+	0	ID=BBa_K115002;Name=BBa_K115002
BBa_K887002	.	sequence_alteration	5352	5357	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K887002	.	ribosome_entry_site	5376	5381	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K887002	.	stem_loop	5330	5347	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K887002	.	stem_loop	5353	5381	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K887002	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K887002	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K887002	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K887002	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K887002	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K887002	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K887002	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K887002	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K887002	.	CDS	6283	7929	.	+	0	ID=BBa_K539714;Name=BBa_K539714
BBa_K887002	.	CDS	6283	7929	.	+	0	ID=annotation2136208;Name=kivd coding sequence;Parent=BBa_K539714
BBa_K887002	.	ribosome_entry_site	3453	3464	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887002	.	conserved_region	3457	3460	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887002	.	terminator	8026	8066	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K887002	.	stem_loop	8033	8052	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K887002	.	stop_codon	8059	8059	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K887002	.	polyA_site	8053	8066	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K887002	.	engineered_region	8026	8066	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K887002	.	terminator	6081	6193	.	+	0	ID=BBa_J61048;Name=BBa_J61048
BBa_K887002	.	CDS	5388	6072	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K887002	.	tag	6008	6041	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K887002	.	engineered_tag	6048	6072	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K887002	.	CDS	5391	6007	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
>BBa_K887002
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgttgacaaa
agcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtc
acacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcg
ttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagt
cacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgctt
gctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgatta
caaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagg
gcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgtt
gcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctg
tcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttcc
atttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttc
cgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatc
cgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttacca
gcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgca
gagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcag
acagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcgg
ttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaaca
ctcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacg
gcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacga
cagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgat
atcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtc
aaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaa
gcttccgaaagaatttggggaactcatgaaaacgaaagctctctagtactagagaaagaggagaaatactagatggcta
actacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgc
cgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaac
atgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaag
cgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccgga
caagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttc
aacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaag
tgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaagg
catggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaa
gtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagc
tggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaa
acagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctg
aaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcgg
actgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagta
tgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactg
gcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattg
ccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgtt
ctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaa
ggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaac
tgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatactagagaaagaggagaaatactagatgcct
aagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccg
acgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcga
tctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgat
gggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatgg
tcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccct
gcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatc
aagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgtt
ccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcct
gtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcatt
gttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttg
aaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagc
ggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacc
cagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttac
tgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccagga
tgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgg
gatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcgg
tgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccgg
cccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggta
gtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgg
gtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctc
accggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggc
attcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgc
cgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggt
gcgcgataaatcgaaactggggggttaatactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtga
ttcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaa
tcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataa
gcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagc
tggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatt
taggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcact
agagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtc
gctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcacc
aaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccg
gcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaa
tgactgtccacgacgctatacccaaaagaaatactagagtccctatcagtgatagagattgacatccctatcagtgata
gagatactgagcactactagagtcacacaggaaagtactagatgtatacagtaggagattacctattagaccgattaca
cgagttaggaattgaagaaatttttggagtccctggagactataacttacaatttttagatcaaattatttcccgcaag
gatatgaaatgggtcggaaatgctaatgaattaaatgcttcatatatggctgatggctatgctcgtactaaaaaagctg
ccgcatttcttacaacctttggagtaggtgaattgagtgcagttaatggattagcaggaagttacgccgaaaatttacc
agtagtagaaatagtgggatcacctacatcaaaagttcaaaatgaaggaaaatttgttcatcatacgctggctgacggt
gattttaaacactttatgaaaatgcacgaacctgttacagcagctcgaactttactgacagcagaaaatgcaaccgttg
aaattgaccgagtactttctgcactattaaaagaaagaaaacctgtctatatcaacttaccagttgatgttgctgctgc
aaaagcagagaaaccctcactccctttgaaaaaagaaaactcaacttcaaatacaagtgaccaagagatcttgaacaaa
attcaagaaagcttgaaaaatgccaaaaaaccaatcgtgattacaggacatgaaataattagttttggcttagaaaaaa
cagtctctcaatttatttcaaagacaaaactacctattacgacattaaactttggaaaaagttcagttgatgaagctct
cccttcatttttaggaatctataatggtaaactctcagagcctaatcttaaagtattcgtggaatcagccgacttcatc
ctgatgcttggagttaaactcacagactcttcaacaggagccttcactcatcatttaaatgaaaataaaatgatttcac
tgaatatagatgaaggaaaaatatttaacgaaagcatccaaaattttgattttgaatccctcatctcctctctcttaga
cctaagcgaaatagaatacaaaggaaaatatatcgataaaaagcaagaagactttgttccatcaaatgcgcttttatca
caagaccgcctatggcaagcagttgaaaacctaactcaaagcaatgaaacaatcgttgctgaacaagggacatcattct
ttggcgcttcatcaattttcttaaaaccaaagagtcattttattggtcaacccttatggggatcaattggatatacatt
cccagcagcattaggaagccaaattgcagataaagaaagcagacaccttttatttattggtgatggttcacttcaactt
acggtgcaagaattaggattagcaatcagagaaaaaattaatccaatttgctttattatcaataatgatggttatacag
tcgaaagagaaattcatggaccaaatcaaagctacaatgatattccaatgtggaattactcaaaattaccagaatcatt
tggagcaacagaagaacgagtagtctcgaaaatcgttagaactgaaaatgaatttgtgtctgtcatgaaagaagctcaa
gcagatccaaatagaatgtactggattgagttaattttggcaaaagaagatgcaccaaaagtactgaaaaaaatgggca
aactatttgctgaacaaaataaatcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata