LOCUS BBa_K887003 9626 bp DNA linear 15-Dec-2024 DEFINITION Plac+B0034+alsS+zif268+B0034+PBS II+ilvC+B0034+HIVC+ilvD+Ptet+B0032 +kivD+B0015 ACCESSION BBa_K887003 VERSION BBa_K887003.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 misc_feature 93..96 /label=conserved RBS 89..100 /label=BBa_B0034 misc_feature 109..402 /label=BBa_K323105 CDS 409..2124 /label=AlsS CDS 409..2124 /label=BBa_K539713 misc_feature 2137..2140 /label=conserved RBS 2133..2144 /label=BBa_B0034 misc_feature 2153..2440 /label=BBa_K323107 CDS 2447..3922 /label=ilvC coding sequence CDS 2447..3922 /label=BBa_K539711 misc_feature 3935..3938 /label=conserved RBS 3931..3942 /label=BBa_B0034 RBS 3956..3962 /label=Elowitz RBS misc_feature 3970..3973 /label=ATG misc_feature 3973..4224 /label=Bba_K165006 misc_feature 4231..5895 /label=crtO misc_feature 3951..5896 /label=BBa_K323128 CDS 5903..7753 /label=ilvD CDS 5903..7753 /label=BBa_K539712 misc_feature 7762..7780 /label=TetR 1 misc_feature 7762..7815 /label=BBa_R0040 promoter 7781..7786 /label=-35 misc_feature 7787..7805 /label=TetR 2 promoter 7804..7809 /label=-10 promoter 7762..7815 /label=BBa_R0040 RBS 7824..7836 /label=RBS-3\Weak RBS 7830..7833 /label=RBS misc_feature 7824..7836 /label=BBa_B0032 RBS 7824..7836 /label=BBa_B0032 CDS 7843..9489 /label=kivd coding sequence CDS 7843..9489 /label=BBa_K539714 stem_loop 9509..9552 /label=stem_loop misc_feature 9498..9577 /label=BBa_B0010 terminator 9498..9577 /label=BBa_B0010 stem_loop 9593..9612 /label=T7 TE misc_feature 9619..9619 /label=stop polyA_site 9613..9626 /label=polya misc_feature 9586..9626 /label=BBa_B0012 terminator 9586..9626 /label=BBa_B0012 terminator 9498..9626 /label=BBa_B0015 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagaa agaggagaaa tactagagcc gggtgaaaaa 121 ccgtatgcat gtccggttga aagctgtgat cgtcgtttta gccgtagtga tgaactgacc 181 cgtcatattc gtattcatac aggtcagaaa ccgtttcagt gtcgtatttg catgcgtaat 241 tttagccgtt cagatcatct gaccacccat attcgtaccc atactggcga aaaaccgttt 301 gcctgtgata tttgtggtcg taaatttgca cgttccgatg aacgtaaacg ccataccaaa 361 attcatacgg gtggtggtag cggtggtggt tcaggtggta gttactagat gttgacaaaa 421 gcaacaaaag aacaaaaatc ccttgtgaaa aacagagggg cggagcttgt tgttgattgc 481 ttagtggagc aaggtgtcac acatgtattt ggcattccag gtgcaaaaat tgatgcggta 541 tttgacgctt tacaagataa aggacctgaa attatcgttg cccggcacga acaaaacgca 601 gcattcatgg cccaagcagt cggccgttta actggaaaac cgggagtcgt gttagtcaca 661 tcaggaccgg gtgcctctaa cttggcaaca ggcctgctga cagcgaacac tgaaggagac 721 cctgtcgttg cgcttgctgg aaacgtgatc cgtgcagatc gtttaaaacg gacacatcaa 781 tctttggata atgcggcgct attccagccg attacaaaat acagtgtaga agttcaagat 841 gtaaaaaata taccggaagc tgttacaaat gcatttagga tagcgtcagc agggcaggct 901 ggggccgctt ttgtgagctt tccgcaagat gttgtgaatg aagtcacaaa tacgaaaaac 961 gtgcgtgctg ttgcagcgcc aaaactcggt ccagcagcag atgatgcaat cagtgcggcc 1021 atagcaaaaa tccaaacagc aaaacttcct gtcgttttgg tcggcatgaa aggcggaaga 1081 ccggaagcaa ttaaggcggt tcgcaagctt ttgaaaaagg ttcagcttcc atttgttgaa 1141 acatatcaag ctgccggtac cctttccaga gatttagagg atcaatattt tggccgtatc 1201 ggtttgttcc gcaaccagcc tggcgattta ctgctagagc aggcagatgt tgttctgacg 1261 atcggctatg acccgattga atatgatccg aaattctgga atatcaatgg agaccggaca 1321 attatccatt tagacgagat tatcgctgac attgatcatg cttaccagcc tgatcttgaa 1381 ttgatcggtg acattccgtc cacgatcaat catatcgaac acgatgctgt gaaagtggaa 1441 tttgcagagc gtgagcagaa aatcctttct gatttaaaac aatatatgca tgaaggtgag 1501 caggtgccag cagattggaa atcagacaga gcgcaccctc ttgaaatcgt taaagagttg 1561 cgtaatgcag tcgatgatca tgttacagta acttgcgata tcggttcgca cgccatttgg 1621 atgtcacgtt atttccgcag ctacgagccg ttaacattaa tgatcagtaa cggtatgcaa 1681 acactcggcg ttgcgcttcc ttgggcaatc ggcgcttcat tggtgaaacc gggagaaaaa 1741 gtggtttctg tctctggtga cggcggtttc ttattctcag caatggaatt agagacagca 1801 gttcgactaa aagcaccaat tgtacacatt gtatggaacg acagcacata tgacatggtt 1861 gcattccagc aattgaaaaa atataaccgt acatctgcgg tcgatttcgg aaatatcgat 1921 atcgtgaaat atgcggaaag cttcggagca actggcttgc gcgtagaatc accagaccag 1981 ctggcagatg ttctgcgtca aggcatgaac gctgaaggtc ctgtcatcat cgatgtcccg 2041 gttgactaca gtgataacat taatttagca agtgacaagc ttccgaaaga atttggggaa 2101 ctcatgaaaa cgaaagctct ctagtactag agaaagagga gaaatactag agccgggtga 2161 aaaaccgtat gcatgtcctg aatgtggtaa aagctttagc cagcgtgcaa atctgcgtgc 2221 acatcagcgt acccatacag gtgaaaagcc ttataaatgc ccagaatgcg gcaaaagctt 2281 ttcacgtagc gatcatctga ccacccatca gcgcacacat actggcgaga aaccttacaa 2341 atgtccagag tgtggtaaat catttagccg tagtgatgtt ctggttcgtc atcagagaac 2401 ccacacgggt ggtggtagcg gtggtggttc aggtggtagt tactagatgg ctaactactt 2461 caatacactg aatctgcgcc agcagctggc acagctgggc aaatgtcgct ttatgggccg 2521 cgatgaattt gccgatggcg cgagctacct tcagggtaaa aaagtagtca tcgtcggctg 2581 tggcgcacag ggtctgaacc agggcctgaa catgcgtgat tctggtctcg atatctccta 2641 cgctctgcgt aaagaagcga ttgccgagaa gcgcgcgtcc tggcgtaaag cgaccgaaaa 2701 tggttttaaa gtgggtactt acgaagaact gatcccacag gcggatctgg tgattaacct 2761 gacgccggac aagcagcact ctgatgtagt gcgcaccgta cagccactga tgaaagacgg 2821 cgcggcgctg ggctactcgc acggtttcaa catcgtcgaa gtgggcgagc agatccgtaa 2881 agatatcacc gtagtgatgg ttgcgccgaa atgcccaggc accgaagtgc gtgaagagta 2941 caaacgtggg ttcggcgtac cgacgctgat tgccgttcac ccggaaaacg atccgaaagg 3001 cgaaggcatg gcgattgcca aagcctgggc ggctgcaacc ggtggtcacc gtgcgggtgt 3061 gctggaatcg tccttcgttg cggaagtgaa atctgatctg atgggcgagc aaaccatcct 3121 gtgcggtatg ttgcaggctg gctctctgct gtgcttcgac aagctggtgg aagaaggtac 3181 cgatccagca tacgcagaaa aactgattca gttcggttgg gaaaccatca ccgaagcact 3241 gaaacagggc ggcatcaccc tgatgatgga ccgtctctct aacccggcga aactgcgtgc 3301 ttatgcgctt tctgaacagc tgaaagagat catggcaccc ctgttccaga aacatatgga 3361 cgacatcatc tccggcgaat tttcttccgg tatgatggcg gactgggcca acgatgataa 3421 gaaactgctg acctggcgtg aagagaccgg caaaaccgcg tttgaaaccg cgccgcagta 3481 tgaaggcaaa atcggcgagc aggagtactt cgataaaggc gtactgatga ttgcgatggt 3541 gaaagcgggc gttgaactgg cgttcgaaac catggtcgat tccggcatca ttgaagagtc 3601 tgcatattat gaatcactgc acgagctgcc gctgattgcc aacaccatcg cccgtaagcg 3661 tctgtacgaa atgaacgtgg ttatctctga taccgctgag tacggtaact atctgttctc 3721 ttacgcttgt gtgccgttgc tgaaaccgtt tatggcagag ctgcaaccgg gcgacctggg 3781 taaagctatt ccggaaggcg cggtagataa cgggcaactg cgtgatgtga acgaagcgat 3841 tcgcagccat gcgattgagc aggtaggtaa gaaactgcgc ggctatatga cagatatgaa 3901 acgtattgct gttgcgggtt aatactagag aaagaggaga aatactagag gaaagaggag 3961 aaatactaga atgcccttcc agtgtcgcat ttgcatgcgg aacttttcgc tcaggacgga 4021 ccttgacagg catacccgta ctcataccgg tgaaaaaccg tttcagtgtc ggatctgtat 4081 gcgaaatttc tccctcagcc agaccttgcg ccgccatcta cgtacgcaca ccggcgagaa 4141 gccattccaa tgccgaatat gcatgcgcaa cttcagtctc aggagcaacc tggggcggca 4201 cctaaaaacc cacacaggag aaaaaactag aggcgggtca ggcgggggct caggcgggtc 4261 aactagaatg attaccaccg atgttgttat tattggcgca ggtcataatg gtctggtttg 4321 tgcagcatat ctgctgcaac gtggtctggg tgttaccctg ctggaaaaac gtgaagttcc 4381 gggtggtgca gcaaccaccg aagcactgat gccggaactg agtccgcagt ttcgttttaa 4441 tcgttgtgcc attgatcatg aatttatttt tctgggtccg gttctgcaag aactgaatct 4501 ggcacagtat ggtctggaat acctgttttg tgatccgagc gttttttgtc cgggtctgga 4561 tggtcaggca tttatgagct atcgtagcct ggaaaaaacc tgtgcacata ttgcaaccta 4621 tagtccgcgt gatgcagaaa aatatcgcca gtttgtgaat tattggaccg atctgctgaa 4681 tgcagttcag cctgcattta atgcacctcc gcaggcactg ctggatctgg cactgaatta 4741 tggttgggaa aatctgaaaa gcgttctggc aattgcaggt agcaaaacca aagcactgga 4801 ttttattcgc accatgattg gttcaccgga agatgttctg aatgaatggt ttgatagcga 4861 acgtgttaaa gcaccgctgg cacgtctgtg tagcgaaatt ggtgcacctc cgagccagaa 4921 aggtagcagc agcggtatga tgatggttgc aatgcgtcat ctggaaggta ttgcacgtcc 4981 gaaaggtggt acaggtgcac tgaccgaagc cctggttaaa ctggttcagg cacagggtgg 5041 taaaattctg accgatcaga ccgttaaacg tgttctggtt gaaaataatc aggccattgg 5101 tgttgaagtt gcaaatggtg aacagtatcg tgccaaaaaa ggcgtgatta gcaatattga 5161 tgcccgtcgt ctgtttctgc aactggttga accgggtgca ctggcaaaag ttaatcagaa 5221 tctgggtgaa cgtctggaac gtcgtaccgt taataataat gaagccattc tgaaaattga 5281 ttgtgcactg agcggtctgc cgcattttac cgcaatggca ggtcctgaag atctgaccgg 5341 caccattctg attgcagata gcgttcgtca tgttgaagaa gcacacgcac tgattgcact 5401 gggtcagatt ccagatgcaa atccgagcct gtatctggat attccgaccg ttctggatcc 5461 gaccatggca cctccgggtc agcataccct gtggattgaa ttttttgcac cgtatcgtat 5521 tgcaggtctg gaaggtacag gtctgatggg tacaggttgg accgatgaac tgaaagaaaa 5581 agttgccgat cgcgtgattg ataaactgac cgattatgca ccgaatctga aaagtctgat 5641 tattggtcgt cgtgttgaaa gtccggcaga actggcacag cgtctgggta gctataatgg 5701 taatgtttat catctggata tgagcctgga tcagatgatg tttctgcgtc cgctgccgga 5761 aattgcaaat tatcagaccc cgattaaaaa tctgtatctg acaggtgcag gcacccatcc 5821 gggtggtagc attagcggta tgccgggtcg taattgtgca cgtgtttttc tgaaacagca 5881 gcgtcgtttt tggtaatact agatgcctaa gtaccgttcc gccaccacca ctcatggtcg 5941 taatatggcg ggtgctcgtg cgctgtggcg cgccaccgga atgaccgacg ccgatttcgg 6001 taagccgatt atcgcggttg tgaactcgtt cacccaattt gtaccgggtc acgtccatct 6061 gcgcgatctc ggtaaactgg tcgccgaaca aattgaagcg gctggcggcg ttgccaaaga 6121 gttcaacacc attgcggtgg atgatgggat tgccatgggc cacgggggga tgctttattc 6181 actgccatct cgcgaactga tcgctgattc cgttgagtat atggtcaacg cccactgcgc 6241 cgacgccatg gtctgcatct ctaactgcga caaaatcacc ccggggatgc tgatggcttc 6301 cctgcgcctg aatattccgg tgatctttgt ttccggcggc ccgatggagg ccgggaaaac 6361 caaactttcc gatcagatca tcaagctcga tctggtcgat gcgatgatcc agggcgcaga 6421 cccgaaagta tctgactccc agagcgatca ggttgaacgt tccgcgtgtc cgacctgcgg 6481 ttcctgctcc gggatgttta ccgctaactc aatgaactgc ctgaccgaag cgctgggcct 6541 gtcgcagccg ggcaacggct cgctgctggc aacccacgcc gaccgtaagc agctgttcct 6601 taatgctggt aaacgcattg ttgaattgac caaacgttat tacgagcaaa acgacgaaag 6661 tgcactgccg cgtaatatcg ccagtaaggc ggcgtttgaa aacgccatga cgctggatat 6721 cgcgatgggt ggatcgacta acaccgtact tcacctgctg gcggcggcgc aggaagcgga 6781 aatcgacttc accatgagtg atatcgataa gctttcccgc aaggttccac agctgtgtaa 6841 agttgcgccg agcacccaga aataccatat ggaagatgtt caccgtgctg gtggtgttat 6901 cggtattctc ggcgaactgg atcgcgcggg gttactgaac cgtgatgtga aaaacgtact 6961 tggcctgacg ttgccgcaaa cgctggaaca atacgacgtt atgctgaccc aggatgacgc 7021 ggtaaaaaat atgttccgcg caggtccagc aggcattcgt accacacagg cattctcgca 7081 agattgccgt tgggatacgc tggacgacga tcgcgccaat ggctgtatcc gctcgctgga 7141 acacgcctac agcaaagacg gcggcctggc ggtgctctac ggtaactttg cggaaaacgg 7201 ctgcatcgtg aaaacggcag gcgtcgatga cagcatcctc aaattcaccg gcccggcgaa 7261 agtgtacgaa agccaggacg atgcggtaga agcgattctc ggcggtaaag ttgtcgccgg 7321 agatgtggta gtaattcgct atgaaggccc gaaaggcggt ccggggatgc aggaaatgct 7381 ctacccaacc agcttcctga aatcaatggg tctcggcaaa gcctgtgcgc tgatcaccga 7441 cggtcgtttc tctggtggca cctctggtct ttccatcggc cacgtctcac cggaagcggc 7501 aagcggcggc agcattggcc tgattgaaga tggtgacctg atcgctatcg acatcccgaa 7561 ccgtggcatt cagttacagg taagcgatgc cgaactggcg gcgcgtcgtg aagcgcagga 7621 cgctcgaggt gacaaagcct ggacgccgaa aaatcgtgaa cgtcaggtct cctttgccct 7681 gcgtgcttat gccagcctgg caaccagcgc cgacaaaggc gcggtgcgcg ataaatcgaa 7741 actggggggt taatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat 7801 agagatactg agcactacta gagtcacaca ggaaagtact agatgtatac agtaggagat 7861 tacctattag accgattaca cgagttagga attgaagaaa tttttggagt ccctggagac 7921 tataacttac aatttttaga tcaaattatt tcccgcaagg atatgaaatg ggtcggaaat 7981 gctaatgaat taaatgcttc atatatggct gatggctatg ctcgtactaa aaaagctgcc 8041 gcatttctta caacctttgg agtaggtgaa ttgagtgcag ttaatggatt agcaggaagt 8101 tacgccgaaa atttaccagt agtagaaata gtgggatcac ctacatcaaa agttcaaaat 8161 gaaggaaaat ttgttcatca tacgctggct gacggtgatt ttaaacactt tatgaaaatg 8221 cacgaacctg ttacagcagc tcgaacttta ctgacagcag aaaatgcaac cgttgaaatt 8281 gaccgagtac tttctgcact attaaaagaa agaaaacctg tctatatcaa cttaccagtt 8341 gatgttgctg ctgcaaaagc agagaaaccc tcactccctt tgaaaaaaga aaactcaact 8401 tcaaatacaa gtgaccaaga gatcttgaac aaaattcaag aaagcttgaa aaatgccaaa 8461 aaaccaatcg tgattacagg acatgaaata attagttttg gcttagaaaa aacagtctct 8521 caatttattt caaagacaaa actacctatt acgacattaa actttggaaa aagttcagtt 8581 gatgaagctc tcccttcatt tttaggaatc tataatggta aactctcaga gcctaatctt 8641 aaagaattcg tggaatcagc cgacttcatc ctgatgcttg gagttaaact cacagactct 8701 tcaacaggag ccttcactca tcatttaaat gaaaataaaa tgatttcact gaatatagat 8761 gaaggaaaaa tatttaacga aagcatccaa aattttgatt ttgaatccct catctcctct 8821 ctcttagacc taagcgaaat agaatacaaa ggaaaatata tcgataaaaa gcaagaagac 8881 tttgttccat caaatgcgct tttatcacaa gaccgcctat ggcaagcagt tgaaaaccta 8941 actcaaagca atgaaacaat cgttgctgaa caagggacat cattctttgg cgcttcatca 9001 attttcttaa aaccaaagag tcattttatt ggtcaaccct tatggggatc aattggatat 9061 acattcccag cagcattagg aagccaaatt gcagataaag aaagcagaca ccttttattt 9121 attggtgatg gttcacttca acttacggtg caagaattag gattagcaat cagagaaaaa 9181 attaatccaa tttgctttat tatcaataat gatggttata cagtcgaaag agaaattcat 9241 ggaccaaatc aaagctacaa tgatattcca atgtggaatt actcaaaatt accagaatca 9301 tttggagcaa cagaagaacg agtagtctcg aaaatcgtta gaactgaaaa tgaatttgtg 9361 tctgtcatga aagaagctca agcagatcca aatagaatgt actggattga gttaattttg 9421 gcaaaagaag atgcaccaaa agtactgaaa aaaatgggca aactatttgc tgaacaaaat 9481 aaatcataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 9541 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 9601 ttcgggtggg cctttctgcg tttata //