##gff-version 3
##sequence-region BBa_K887003 1 9626
BBa_K887003	.	promoter	7762	7815	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K887003	.	engineered_region	7762	7815	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K887003	.	promoter	7781	7786	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K887003	.	promoter	7804	7809	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K887003	.	non_covalent_binding_site	7762	7780	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K887003	.	non_covalent_binding_site	7787	7805	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K887003	.	CDS	5903	7753	.	+	0	ID=BBa_K539712;Name=BBa_K539712
BBa_K887003	.	CDS	5903	7753	.	+	0	ID=annotation2136209;Name=ilvD;Parent=BBa_K539712
BBa_K887003	.	ribosome_entry_site	2133	2144	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887003	.	conserved_region	2137	2140	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887003	.	polypeptide_domain	2153	2440	.	+	0	ID=BBa_K323107;Name=BBa_K323107
BBa_K887003	.	terminator	9498	9626	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K887003	.	terminator	9586	9626	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K887003	.	stem_loop	9593	9612	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K887003	.	stop_codon	9619	9619	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K887003	.	polyA_site	9613	9626	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K887003	.	engineered_region	9586	9626	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K887003	.	terminator	9498	9577	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K887003	.	stem_loop	9509	9552	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K887003	.	engineered_region	9498	9577	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K887003	.	ribosome_entry_site	89	100	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887003	.	conserved_region	93	96	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887003	.	CDS	2447	3922	.	+	0	ID=BBa_K539711;Name=BBa_K539711
BBa_K887003	.	CDS	2447	3922	.	+	0	ID=annotation2136206;Name=ilvC coding sequence;Parent=BBa_K539711
BBa_K887003	.	polypeptide_domain	109	402	.	+	0	ID=BBa_K323105;Name=BBa_K323105
BBa_K887003	.	ribosome_entry_site	3931	3942	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887003	.	conserved_region	3935	3938	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887003	.	CDS	7843	9489	.	+	0	ID=BBa_K539714;Name=BBa_K539714
BBa_K887003	.	CDS	7843	9489	.	+	0	ID=annotation2136208;Name=kivd coding sequence;Parent=BBa_K539714
BBa_K887003	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K887003	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K887003	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K887003	.	ribosome_entry_site	7824	7836	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K887003	.	engineered_region	7824	7836	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K887003	.	ribosome_entry_site	7830	7833	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K887003	.	ribosome_entry_site	7824	7836	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K887003	.	engineered_region	3951	5896	.	+	0	ID=BBa_K323128;Name=BBa_K323128
BBa_K887003	.	sequence_feature	4231	5895	.	+	0	ID=annotation2099955;Name=crtO;Parent=BBa_K323128
BBa_K887003	.	ribosome_entry_site	3956	3962	.	+	0	ID=annotation2099881;Name=Elowitz RBS;Parent=BBa_K323128
BBa_K887003	.	start_codon	3970	3973	.	+	0	ID=annotation2099882;Name=ATG;Parent=BBa_K323128
BBa_K887003	.	engineered_region	3973	4224	.	+	0	ID=annotation2099883;Name=Bba_K165006;Parent=BBa_K323128
BBa_K887003	.	CDS	409	2124	.	+	0	ID=BBa_K539713;Name=BBa_K539713
BBa_K887003	.	CDS	409	2124	.	+	0	ID=annotation2136210;Name=AlsS;Parent=BBa_K539713
>BBa_K887003
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagaaagaggagaaatactagagccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttt
tagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgt
aattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtg
gtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcagg
tggtagttactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgtt
gattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttac
aagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaac
tggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacact
gaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggata
atgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaa
tgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcaca
aatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaa
aaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaa
gcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaa
tattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcg
gctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattat
cgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacac
gatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagc
aggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatca
tgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacatta
atgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaa
aagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcacc
aattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatct
gcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccag
accagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtga
taacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctagtactagaga
aagaggagaaatactagagccgggtgaaaaaccgtatgcatgtcctgaatgtggtaaaagctttagccagcgtgcaaat
ctgcgtgcacatcagcgtacccatacaggtgaaaagccttataaatgcccagaatgcggcaaaagcttttcacgtagcg
atcatctgaccacccatcagcgcacacatactggcgagaaaccttacaaatgtccagagtgtggtaaatcatttagccg
tagtgatgttctggttcgtcatcagagaacccacacgggtggtggtagcggtggtggttcaggtggtagttactagatg
gctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaat
ttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcct
gaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgt
aaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgc
cggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacgg
tttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcacc
gaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcg
aaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgc
ggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgac
aagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcac
tgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaaca
gctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatg
gcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgc
agtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttga
actggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctg
attgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatc
tgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattcc
ggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaag
aaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatactagagaaagaggagaaatactagag
gaaagaggagaaatactagaatgcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacag
gcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttg
cgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagca
acctggggcggcacctaaaaacccacacaggagaaaaaactagaggcgggtcaggcgggggctcaggcgggtcaactag
aatgattaccaccgatgttgttattattggcgcaggtcataatggtctggtttgtgcagcatatctgctgcaacgtggt
ctgggtgttaccctgctggaaaaacgtgaagttccgggtggtgcagcaaccaccgaagcactgatgccggaactgagtc
cgcagtttcgttttaatcgttgtgccattgatcatgaatttatttttctgggtccggttctgcaagaactgaatctggc
acagtatggtctggaatacctgttttgtgatccgagcgttttttgtccgggtctggatggtcaggcatttatgagctat
cgtagcctggaaaaaacctgtgcacatattgcaacctatagtccgcgtgatgcagaaaaatatcgccagtttgtgaatt
attggaccgatctgctgaatgcagttcagcctgcatttaatgcacctccgcaggcactgctggatctggcactgaatta
tggttgggaaaatctgaaaagcgttctggcaattgcaggtagcaaaaccaaagcactggattttattcgcaccatgatt
ggttcaccggaagatgttctgaatgaatggtttgatagcgaacgtgttaaagcaccgctggcacgtctgtgtagcgaaa
ttggtgcacctccgagccagaaaggtagcagcagcggtatgatgatggttgcaatgcgtcatctggaaggtattgcacg
tccgaaaggtggtacaggtgcactgaccgaagccctggttaaactggttcaggcacagggtggtaaaattctgaccgat
cagaccgttaaacgtgttctggttgaaaataatcaggccattggtgttgaagttgcaaatggtgaacagtatcgtgcca
aaaaaggcgtgattagcaatattgatgcccgtcgtctgtttctgcaactggttgaaccgggtgcactggcaaaagttaa
tcagaatctgggtgaacgtctggaacgtcgtaccgttaataataatgaagccattctgaaaattgattgtgcactgagc
ggtctgccgcattttaccgcaatggcaggtcctgaagatctgaccggcaccattctgattgcagatagcgttcgtcatg
ttgaagaagcacacgcactgattgcactgggtcagattccagatgcaaatccgagcctgtatctggatattccgaccgt
tctggatccgaccatggcacctccgggtcagcataccctgtggattgaattttttgcaccgtatcgtattgcaggtctg
gaaggtacaggtctgatgggtacaggttggaccgatgaactgaaagaaaaagttgccgatcgcgtgattgataaactga
ccgattatgcaccgaatctgaaaagtctgattattggtcgtcgtgttgaaagtccggcagaactggcacagcgtctggg
tagctataatggtaatgtttatcatctggatatgagcctggatcagatgatgtttctgcgtccgctgccggaaattgca
aattatcagaccccgattaaaaatctgtatctgacaggtgcaggcacccatccgggtggtagcattagcggtatgccgg
gtcgtaattgtgcacgtgtttttctgaaacagcagcgtcgtttttggtaatactagatgcctaagtaccgttccgccac
caccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaag
ccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcg
ccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggcca
cggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgcc
gacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccgg
tgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcga
tgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgc
ggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacg
gctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacg
ttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctg
gatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcacca
tgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatgga
agatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaa
aacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaata
tgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacga
tcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaacttt
gcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacg
aaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaagg
cccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgt
gcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcg
gcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaag
cgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgt
caggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaac
tggggggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag
agtcacacaggaaagtactagatgtatacagtaggagattacctattagaccgattacacgagttaggaattgaagaaa
tttttggagtccctggagactataacttacaatttttagatcaaattatttcccgcaaggatatgaaatgggtcggaaa
tgctaatgaattaaatgcttcatatatggctgatggctatgctcgtactaaaaaagctgccgcatttcttacaaccttt
ggagtaggtgaattgagtgcagttaatggattagcaggaagttacgccgaaaatttaccagtagtagaaatagtgggat
cacctacatcaaaagttcaaaatgaaggaaaatttgttcatcatacgctggctgacggtgattttaaacactttatgaa
aatgcacgaacctgttacagcagctcgaactttactgacagcagaaaatgcaaccgttgaaattgaccgagtactttct
gcactattaaaagaaagaaaacctgtctatatcaacttaccagttgatgttgctgctgcaaaagcagagaaaccctcac
tccctttgaaaaaagaaaactcaacttcaaatacaagtgaccaagagatcttgaacaaaattcaagaaagcttgaaaaa
tgccaaaaaaccaatcgtgattacaggacatgaaataattagttttggcttagaaaaaacagtctctcaatttatttca
aagacaaaactacctattacgacattaaactttggaaaaagttcagttgatgaagctctcccttcatttttaggaatct
ataatggtaaactctcagagcctaatcttaaagaattcgtggaatcagccgacttcatcctgatgcttggagttaaact
cacagactcttcaacaggagccttcactcatcatttaaatgaaaataaaatgatttcactgaatatagatgaaggaaaa
atatttaacgaaagcatccaaaattttgattttgaatccctcatctcctctctcttagacctaagcgaaatagaataca
aaggaaaatatatcgataaaaagcaagaagactttgttccatcaaatgcgcttttatcacaagaccgcctatggcaagc
agttgaaaacctaactcaaagcaatgaaacaatcgttgctgaacaagggacatcattctttggcgcttcatcaattttc
ttaaaaccaaagagtcattttattggtcaacccttatggggatcaattggatatacattcccagcagcattaggaagcc
aaattgcagataaagaaagcagacaccttttatttattggtgatggttcacttcaacttacggtgcaagaattaggatt
agcaatcagagaaaaaattaatccaatttgctttattatcaataatgatggttatacagtcgaaagagaaattcatgga
ccaaatcaaagctacaatgatattccaatgtggaattactcaaaattaccagaatcatttggagcaacagaagaacgag
tagtctcgaaaatcgttagaactgaaaatgaatttgtgtctgtcatgaaagaagctcaagcagatccaaatagaatgta
ctggattgagttaattttggcaaaagaagatgcaccaaaagtactgaaaaaaatgggcaaactatttgctgaacaaaat
aaatcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata