BBa_K887003
type: ComponentDefinition
persistent identity: BBa K887003
display id: BBa_K887003
version: 1
was generated by: iGEM to SBOL conversion
description: Plac+B0034+alsS+zif268+B0034+PBS II+ilvC+B0034+HIVC+ilvD+Ptet+B0032+kivD+B0015
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: The circuit is optimized from 2011 NCTU_FORMOSA.
owned by: james
owned by: myers
mutable description: This part contain four enzymes. Each of the former three enzymes(alsS, ilvC& ilvD) linked with a zinc finger. The three enzymes could convert pyruvate into 2-ketoisoalerate(the Intermediate of isobutanol). The final enzyme(kivD)could change 2-ketoisoalerate into isobutyraldehyde, which would be converted into isobutanol by ADH in E.coli. This is a complete circuit to produce isobutanol.
discontinued: true
dominant: false
experience: experience/None
group u list: _1150_
m user id: 0
owner id: 14175
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: We add zinc fingers in front of enzymes in the circuit, and therefore the enzymes could bind the DNA program in order. It help enhance the efficiency of isobutanol production .
star: false
creator: WU, CHENG-HSUAN
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K539712
component: BBa_K323128
component: BBa_B0034
component: BBa_K539711
component: BBa_K323107
component: BBa_K539713
component: BBa_K323105
component: BBa_B0015
component: BBa_K539714
component: BBa_B0032
component: BBa_R0040
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K323105
sequence annotation: BBa_K539713
sequence annotation: BBa_K323107
sequence annotation: BBa_K539711
sequence annotation: BBa_K323128
sequence annotation: BBa_K539712
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_K539714
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K887003_sequence/1

BBa_K539712
type: Component
persistent identity: component2189220
display id: component2189220
version: 1
top level: BBa_K887003
definition: BBa_K539712
access: public

BBa_K323128
type: Component
persistent identity: component2189218
display id: component2189218
version: 1
top level: BBa_K887003
definition: BBa_K323128
access: public

BBa_B0034
type: Component
persistent identity: component2189213
display id: component2189213
version: 1
top level: BBa_K887003
definition: BBa_B0034
access: public

BBa_K539711
type: Component
persistent identity: component2189211
display id: component2189211
version: 1
top level: BBa_K887003
definition: BBa_K539711
access: public

BBa_K323107
type: Component
persistent identity: component2189209
display id: component2189209
version: 1
top level: BBa_K887003
definition: BBa_K323107
access: public

BBa_B0034
type: Component
persistent identity: component2189208
display id: component2189208
version: 1
top level: BBa_K887003
definition: BBa_B0034
access: public

BBa_K539713
type: Component
persistent identity: component2189206
display id: component2189206
version: 1
top level: BBa_K887003
definition: BBa_K539713
access: public

BBa_K323105
type: Component
persistent identity: component2189204
display id: component2189204
version: 1
top level: BBa_K887003
definition: BBa_K323105
access: public

BBa_B0015
type: Component
persistent identity: component2189237
display id: component2189237
version: 1
top level: BBa_K887003
definition: BBa_B0015
access: public

BBa_K539714
type: Component
persistent identity: component2189230
display id: component2189230
version: 1
top level: BBa_K887003
definition: BBa_K539714
access: public

BBa_B0032
type: Component
persistent identity: component2189227
display id: component2189227
version: 1
top level: BBa_K887003
definition: BBa_B0032
access: public

BBa_R0040
type: Component
persistent identity: component2189221
display id: component2189221
version: 1
top level: BBa_K887003
definition: p(tetR)
access: public

BBa_B0034
type: Component
persistent identity: component2189203
display id: component2189203
version: 1
top level: BBa_K887003
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2189200
display id: component2189200
version: 1
top level: BBa_K887003
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2189200
display id: annotation2189200
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189200/range2189200/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K887003/annotation2189200/range2189200/1
type: Range
persistent identity: range2189200
display id: range2189200
version: 1
top level: BBa_K887003
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2189203
display id: annotation2189203
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189203/range2189203/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887003/annotation2189203/range2189203/1
type: Range
persistent identity: range2189203
display id: range2189203
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 89
end: 100
orientation: inline

BBa_K323105
type: SequenceAnnotation
persistent identity: annotation2189204
display id: annotation2189204
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189204/range2189204/1
component: BBa_K323105

https://synbiohub.org/public/igem/BBa_K887003/annotation2189204/range2189204/1
type: Range
persistent identity: range2189204
display id: range2189204
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 109
end: 402
orientation: inline

BBa_K539713
type: SequenceAnnotation
persistent identity: annotation2189206
display id: annotation2189206
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189206/range2189206/1
component: BBa_K539713

https://synbiohub.org/public/igem/BBa_K887003/annotation2189206/range2189206/1
type: Range
persistent identity: range2189206
display id: range2189206
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 409
end: 2124
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2189208
display id: annotation2189208
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189208/range2189208/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887003/annotation2189208/range2189208/1
type: Range
persistent identity: range2189208
display id: range2189208
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 2133
end: 2144
orientation: inline

BBa_K323107
type: SequenceAnnotation
persistent identity: annotation2189209
display id: annotation2189209
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189209/range2189209/1
component: BBa_K323107

https://synbiohub.org/public/igem/BBa_K887003/annotation2189209/range2189209/1
type: Range
persistent identity: range2189209
display id: range2189209
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 2153
end: 2440
orientation: inline

BBa_K539711
type: SequenceAnnotation
persistent identity: annotation2189211
display id: annotation2189211
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189211/range2189211/1
component: BBa_K539711

https://synbiohub.org/public/igem/BBa_K887003/annotation2189211/range2189211/1
type: Range
persistent identity: range2189211
display id: range2189211
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 2447
end: 3922
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2189213
display id: annotation2189213
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189213/range2189213/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K887003/annotation2189213/range2189213/1
type: Range
persistent identity: range2189213
display id: range2189213
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 3931
end: 3942
orientation: inline

BBa_K323128
type: SequenceAnnotation
persistent identity: annotation2189218
display id: annotation2189218
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189218/range2189218/1
component: BBa_K323128

https://synbiohub.org/public/igem/BBa_K887003/annotation2189218/range2189218/1
type: Range
persistent identity: range2189218
display id: range2189218
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 3951
end: 5896
orientation: inline

BBa_K539712
type: SequenceAnnotation
persistent identity: annotation2189220
display id: annotation2189220
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189220/range2189220/1
component: BBa_K539712

https://synbiohub.org/public/igem/BBa_K887003/annotation2189220/range2189220/1
type: Range
persistent identity: range2189220
display id: range2189220
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 5903
end: 7753
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2189221
display id: annotation2189221
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189221/range2189221/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K887003/annotation2189221/range2189221/1
type: Range
persistent identity: range2189221
display id: range2189221
version: 1
top level: BBa_K887003
direction: direction/forward
start: 7762
end: 7815
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2189227
display id: annotation2189227
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189227/range2189227/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K887003/annotation2189227/range2189227/1
type: Range
persistent identity: range2189227
display id: range2189227
version: 1
top level: BBa_K887003
direction: direction/forward
start: 7824
end: 7836
orientation: inline

BBa_K539714
type: SequenceAnnotation
persistent identity: annotation2189230
display id: annotation2189230
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189230/range2189230/1
component: BBa_K539714

https://synbiohub.org/public/igem/BBa_K887003/annotation2189230/range2189230/1
type: Range
persistent identity: range2189230
display id: range2189230
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 7843
end: 9489
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2189237
display id: annotation2189237
version: 1
top level: BBa_K887003
location: https://synbiohub.org/public/igem/BBa_K887003/annotation2189237/range2189237/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K887003/annotation2189237/range2189237/1
type: Range
persistent identity: range2189237
display id: range2189237
version: 1
top level: BBa_K887003
direction: direction/reverse
start: 9498
end: 9626
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K323107
type: ComponentDefinition
persistent identity: BBa K323107
display id: BBa_K323107
version: 1
was generated by: iGEM to SBOL conversion
description: PBSII zinc finger + linker
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Tina Lebar
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K323107_sequence/1

BBa_K323105
type: ComponentDefinition
persistent identity: BBa K323105
display id: BBa_K323105
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268 zinc finger + linker
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Tina Lebar
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K323105_sequence/1

BBa_K539713
type: ComponentDefinition
persistent identity: BBa K539713
display id: BBa_K539713
version: 1
was generated by: iGEM to SBOL conversion
description: AlsS (alpha-acetolactate synthase protein)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Bacillus subtilis.
owned by: james
owned by: myers
mutable description: This is the coding region of AlsS from Bacillus subtilis. It encodes alpha-acetolactate synthase protein which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AlsS
sequence: https://synbiohub.org/public/igem/BBa_K539713_sequence/1

AlsS
type: SequenceAnnotation
persistent identity: annotation2136210
display id: annotation2136210
version: 1
top level: BBa_K539713
location: https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
type: Range
persistent identity: range2136210
display id: range2136210
version: 1
top level: BBa_K539713
direction: direction/forward
start: 1
end: 1716
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K323128
type: ComponentDefinition
persistent identity: BBa K323128
display id: BBa_K323128
version: 1
was generated by: iGEM to SBOL conversion
description: HivC::crtO
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Tina Ilc
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: Elowitz RBS
sequence annotation: ATG
sequence annotation: Bba_K165006
sequence annotation: crtO
sequence: https://synbiohub.org/public/igem/BBa_K323128_sequence/1

Elowitz RBS
type: SequenceAnnotation
persistent identity: annotation2099881
display id: annotation2099881
version: 1
top level: BBa_K323128
location: https://synbiohub.org/public/igem/BBa_K323128/annotation2099881/range2099881/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K323128/annotation2099881/range2099881/1
type: Range
persistent identity: range2099881
display id: range2099881
version: 1
top level: BBa_K323128
direction: direction/forward
start: 6
end: 12
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2099882
display id: annotation2099882
version: 1
top level: BBa_K323128
location: https://synbiohub.org/public/igem/BBa_K323128/annotation2099882/range2099882/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K323128/annotation2099882/range2099882/1
type: Range
persistent identity: range2099882
display id: range2099882
version: 1
top level: BBa_K323128
direction: direction/forward
start: 20
end: 23
orientation: inline

Bba_K165006
type: SequenceAnnotation
persistent identity: annotation2099883
display id: annotation2099883
version: 1
top level: BBa_K323128
location: https://synbiohub.org/public/igem/BBa_K323128/annotation2099883/range2099883/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K323128/annotation2099883/range2099883/1
type: Range
persistent identity: range2099883
display id: range2099883
version: 1
top level: BBa_K323128
direction: direction/forward
start: 23
end: 274
orientation: inline

crtO
type: SequenceAnnotation
persistent identity: annotation2099955
display id: annotation2099955
version: 1
top level: BBa_K323128
location: https://synbiohub.org/public/igem/BBa_K323128/annotation2099955/range2099955/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K323128/annotation2099955/range2099955/1
type: Range
persistent identity: range2099955
display id: range2099955
version: 1
top level: BBa_K323128
direction: direction/forward
start: 281
end: 1945
orientation: inline

BBa_K539714
type: ComponentDefinition
persistent identity: BBa K539714
display id: BBa_K539714
version: 1
was generated by: iGEM to SBOL conversion
description: kivd (alpha-ketoisovalerate decarboxylase)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:38Z
mutable provenance: It is cloned form Lactococcus lactis subsp. lactis KF147
owned by: james
owned by: myers
mutable description: kivd is the coding sequence from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This is part of the isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to design the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region.However, there is one RE site, EcoR1, in kivd. The RE site in kivd has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: kivd coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K539714_sequence/1

kivd coding sequence
type: SequenceAnnotation
persistent identity: annotation2136208
display id: annotation2136208
version: 1
top level: BBa_K539714
location: https://synbiohub.org/public/igem/BBa_K539714/annotation2136208/range2136208/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539714/annotation2136208/range2136208/1
type: Range
persistent identity: range2136208
display id: range2136208
version: 1
top level: BBa_K539714
direction: direction/forward
start: 1
end: 1647
orientation: inline

BBa_K539711
type: ComponentDefinition
persistent identity: BBa K539711
display id: BBa_K539711
version: 1
was generated by: iGEM to SBOL conversion
description: ilvC (ketol-acid reductoisomerase NAD(P)-binding)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding region of ilvC from Escherichia coli str. K-12 substr(MG1655). It encodes ketol-acid reductoisomerase NAD(P)-binding which is part of the isobutanol biosynthesis pathway.
discontinued: false
dominant: false
experience: experience/None
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding gene, we have to delete those RE site in kivd. The RE site in the part has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ilvC coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K539711_sequence/1

ilvC coding sequence
type: SequenceAnnotation
persistent identity: annotation2136206
display id: annotation2136206
version: 1
top level: BBa_K539711
location: https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
type: Range
persistent identity: range2136206
display id: range2136206
version: 1
top level: BBa_K539711
direction: direction/forward
start: 1
end: 1476
orientation: inline

BBa_K539712
type: ComponentDefinition
persistent identity: BBa K539712
display id: BBa_K539712
version: 1
was generated by: iGEM to SBOL conversion
description: ilvD (dihydroxyacid dehydratase)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding sequence of ilvD form Escherichia coli str. K-12 substr. MG1655. It encodes dihydroxyacid dehydratase which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There is one Pst1 in original sequence of ilvD. However, the RE site in ilvD has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ilvD
sequence: https://synbiohub.org/public/igem/BBa_K539712_sequence/1

ilvD
type: SequenceAnnotation
persistent identity: annotation2136209
display id: annotation2136209
version: 1
top level: BBa_K539712
location: https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
type: Range
persistent identity: range2136209
display id: range2136209
version: 1
top level: BBa_K539712
direction: direction/forward
start: 1
end: 1851
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K887003_sequence/1
type: Sequence
persistent identity: BBa K887003 sequence
display id: BBa_K887003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaagaggagaaatactagagccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcaggtggtagttactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K323107_sequence/1
type: Sequence
persistent identity: BBa K323107 sequence
display id: BBa_K323107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgggtgaaaaaccgtatgcatgtcctgaatgtggtaaaagctttagccagcgtgcaaatctgcgtgcacatcagcgtacccatacaggtgaaaagccttataaatgcccagaatgcggcaaaagcttttcacgtagcgatcatctgaccacccatcagcgcacacatactggcgagaaaccttacaaatgtccagagtgtggtaaatcatttagccgtagtgatgttctggttcgtcatcagagaacccacacgggtggtggtagcggtggtggttcaggtggtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K323105_sequence/1
type: Sequence
persistent identity: BBa K323105 sequence
display id: BBa_K323105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgggtgaaaaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattcatacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcgtacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccataccaaaattcatacgggtggtggtagcggtggtggttcaggtggtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539713_sequence/1
type: Sequence
persistent identity: BBa K539713 sequence
display id: BBa_K539713_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K323128_sequence/1
type: Sequence
persistent identity: BBa K323128 sequence
display id: BBa_K323128_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaagaggagaaatactagaatgcccttccagtgtcgcatttgcatgcggaacttttcgctcaggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttctccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgcaacttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaaactagaggcgggtcaggcgggggctcaggcgggtcaactagaatgattaccaccgatgttgttattattggcgcaggtcataatggtctggtttgtgcagcatatctgctgcaacgtggtctgggtgttaccctgctggaaaaacgtgaagttccgggtggtgcagcaaccaccgaagcactgatgccggaactgagtccgcagtttcgttttaatcgttgtgccattgatcatgaatttatttttctgggtccggttctgcaagaactgaatctggcacagtatggtctggaatacctgttttgtgatccgagcgttttttgtccgggtctggatggtcaggcatttatgagctatcgtagcctggaaaaaacctgtgcacatattgcaacctatagtccgcgtgatgcagaaaaatatcgccagtttgtgaattattggaccgatctgctgaatgcagttcagcctgcatttaatgcacctccgcaggcactgctggatctggcactgaattatggttgggaaaatctgaaaagcgttctggcaattgcaggtagcaaaaccaaagcactggattttattcgcaccatgattggttcaccggaagatgttctgaatgaatggtttgatagcgaacgtgttaaagcaccgctggcacgtctgtgtagcgaaattggtgcacctccgagccagaaaggtagcagcagcggtatgatgatggttgcaatgcgtcatctggaaggtattgcacgtccgaaaggtggtacaggtgcactgaccgaagccctggttaaactggttcaggcacagggtggtaaaattctgaccgatcagaccgttaaacgtgttctggttgaaaataatcaggccattggtgttgaagttgcaaatggtgaacagtatcgtgccaaaaaaggcgtgattagcaatattgatgcccgtcgtctgtttctgcaactggttgaaccgggtgcactggcaaaagttaatcagaatctgggtgaacgtctggaacgtcgtaccgttaataataatgaagccattctgaaaattgattgtgcactgagcggtctgccgcattttaccgcaatggcaggtcctgaagatctgaccggcaccattctgattgcagatagcgttcgtcatgttgaagaagcacacgcactgattgcactgggtcagattccagatgcaaatccgagcctgtatctggatattccgaccgttctggatccgaccatggcacctccgggtcagcataccctgtggattgaattttttgcaccgtatcgtattgcaggtctggaaggtacaggtctgatgggtacaggttggaccgatgaactgaaagaaaaagttgccgatcgcgtgattgataaactgaccgattatgcaccgaatctgaaaagtctgattattggtcgtcgtgttgaaagtccggcagaactggcacagcgtctgggtagctataatggtaatgtttatcatctggatatgagcctggatcagatgatgtttctgcgtccgctgccggaaattgcaaattatcagaccccgattaaaaatctgtatctgacaggtgcaggcacccatccgggtggtagcattagcggtatgccgggtcgtaattgtgcacgtgtttttctgaaacagcagcgtcgtttttggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539714_sequence/1
type: Sequence
persistent identity: BBa K539714 sequence
display id: BBa_K539714_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtatacagtaggagattacctattagaccgattacacgagttaggaattgaagaaatttttggagtccctggagactataacttacaatttttagatcaaattatttcccgcaaggatatgaaatgggtcggaaatgctaatgaattaaatgcttcatatatggctgatggctatgctcgtactaaaaaagctgccgcatttcttacaacctttggagtaggtgaattgagtgcagttaatggattagcaggaagttacgccgaaaatttaccagtagtagaaatagtgggatcacctacatcaaaagttcaaaatgaaggaaaatttgttcatcatacgctggctgacggtgattttaaacactttatgaaaatgcacgaacctgttacagcagctcgaactttactgacagcagaaaatgcaaccgttgaaattgaccgagtactttctgcactattaaaagaaagaaaacctgtctatatcaacttaccagttgatgttgctgctgcaaaagcagagaaaccctcactccctttgaaaaaagaaaactcaacttcaaatacaagtgaccaagagatcttgaacaaaattcaagaaagcttgaaaaatgccaaaaaaccaatcgtgattacaggacatgaaataattagttttggcttagaaaaaacagtctctcaatttatttcaaagacaaaactacctattacgacattaaactttggaaaaagttcagttgatgaagctctcccttcatttttaggaatctataatggtaaactctcagagcctaatcttaaagtattcgtggaatcagccgacttcatcctgatgcttggagttaaactcacagactcttcaacaggagccttcactcatcatttaaatgaaaataaaatgatttcactgaatatagatgaaggaaaaatatttaacgaaagcatccaaaattttgattttgaatccctcatctcctctctcttagacctaagcgaaatagaatacaaaggaaaatatatcgataaaaagcaagaagactttgttccatcaaatgcgcttttatcacaagaccgcctatggcaagcagttgaaaacctaactcaaagcaatgaaacaatcgttgctgaacaagggacatcattctttggcgcttcatcaattttcttaaaaccaaagagtcattttattggtcaacccttatggggatcaattggatatacattcccagcagcattaggaagccaaattgcagataaagaaagcagacaccttttatttattggtgatggttcacttcaacttacggtgcaagaattaggattagcaatcagagaaaaaattaatccaatttgctttattatcaataatgatggttatacagtcgaaagagaaattcatggaccaaatcaaagctacaatgatattccaatgtggaattactcaaaattaccagaatcatttggagcaacagaagaacgagtagtctcgaaaatcgttagaactgaaaatgaatttgtgtctgtcatgaaagaagctcaagcagatccaaatagaatgtactggattgagttaattttggcaaaagaagatgcaccaaaagtactgaaaaaaatgggcaaactatttgctgaacaaaataaatcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539711_sequence/1
type: Sequence
persistent identity: BBa K539711 sequence
display id: BBa_K539711_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539712_sequence/1
type: Sequence
persistent identity: BBa K539712 sequence
display id: BBa_K539712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z