##gff-version 3
##sequence-region BBa_K887004 1 8924
BBa_K887004	.	terminator	6939	7051	.	+	0	ID=BBa_J61048;Name=BBa_J61048
BBa_K887004	.	ribosome_entry_site	4051	4062	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887004	.	conserved_region	4055	4058	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887004	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K887004	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K887004	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K887004	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K887004	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K887004	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K887004	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K887004	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K887004	.	CDS	6246	6930	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K887004	.	engineered_tag	6906	6930	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K887004	.	tag	6866	6899	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K887004	.	CDS	6249	6865	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K887004	.	CDS	529	2244	.	+	0	ID=BBa_K539713;Name=BBa_K539713
BBa_K887004	.	CDS	529	2244	.	+	0	ID=annotation2136210;Name=AlsS;Parent=BBa_K539713
BBa_K887004	.	polypeptide_domain	229	522	.	+	0	ID=BBa_K323105;Name=BBa_K323105
BBa_K887004	.	promoter	7060	7113	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K887004	.	promoter	7102	7107	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K887004	.	promoter	7079	7084	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K887004	.	non_covalent_binding_site	7085	7103	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K887004	.	engineered_region	7060	7113	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K887004	.	non_covalent_binding_site	7060	7078	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K887004	.	terminator	8796	8924	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K887004	.	terminator	8796	8875	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K887004	.	stem_loop	8807	8850	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K887004	.	engineered_region	8796	8875	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K887004	.	terminator	8884	8924	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K887004	.	stem_loop	8891	8910	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K887004	.	stop_codon	8917	8917	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K887004	.	polyA_site	8911	8924	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K887004	.	engineered_region	8884	8924	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K887004	.	CDS	4329	6179	.	+	0	ID=BBa_K539712;Name=BBa_K539712
BBa_K887004	.	CDS	4329	6179	.	+	0	ID=annotation2136209;Name=ilvD;Parent=BBa_K539712
BBa_K887004	.	polypeptide_domain	4071	4322	.	+	0	ID=BBa_K165006;Name=BBa_K165006
BBa_K887004	.	mature_transcript_region	6188	6239	.	+	0	ID=BBa_K115002;Name=BBa_K115002
BBa_K887004	.	stem_loop	6188	6205	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K887004	.	ribosome_entry_site	6234	6239	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K887004	.	sequence_alteration	6210	6215	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K887004	.	stem_loop	6211	6239	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K887004	.	CDS	2567	4042	.	+	0	ID=BBa_K539711;Name=BBa_K539711
BBa_K887004	.	CDS	2567	4042	.	+	0	ID=annotation2136206;Name=ilvC coding sequence;Parent=BBa_K539711
BBa_K887004	.	CDS	7141	8787	.	+	0	ID=BBa_K539714;Name=BBa_K539714
BBa_K887004	.	CDS	7141	8787	.	+	0	ID=annotation2136208;Name=kivd coding sequence;Parent=BBa_K539714
BBa_K887004	.	ribosome_entry_site	7122	7134	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K887004	.	ribosome_entry_site	7122	7134	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K887004	.	engineered_region	7122	7134	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K887004	.	ribosome_entry_site	7128	7131	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K887004	.	ribosome_entry_site	2253	2264	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887004	.	conserved_region	2257	2260	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887004	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K887004	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K887004	.	polypeptide_domain	2273	2560	.	+	0	ID=BBa_K323107;Name=BBa_K323107
>BBa_K887004
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagccgggtgaa
aaaccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagtgatgaactgacccgtcatattcgtattc
atacaggtcagaaaccgtttcagtgtcgtatttgcatgcgtaattttagccgttcagatcatctgaccacccatattcg
tacccatactggcgaaaaaccgtttgcctgtgatatttgtggtcgtaaatttgcacgttccgatgaacgtaaacgccat
accaaaattcatacgggtggtggtagcggtggtggttcaggtggtagttactagatgttgacaaaagcaacaaaagaac
aaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttgg
cattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaa
caaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgg
gtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgat
ccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgta
gaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccg
cttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaact
cggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggc
atgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacat
atcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctgg
cgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaat
atcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaat
tgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaa
aatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccct
cttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccattt
ggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgct
tccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattc
tcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgaca
tggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgc
ggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgct
gaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaat
ttggggaactcatgaaaacgaaagctctctagtactagagaaagaggagaaatactagagccgggtgaaaaaccgtatg
catgtcctgaatgtggtaaaagctttagccagcgtgcaaatctgcgtgcacatcagcgtacccatacaggtgaaaagcc
ttataaatgcccagaatgcggcaaaagcttttcacgtagcgatcatctgaccacccatcagcgcacacatactggcgag
aaaccttacaaatgtccagagtgtggtaaatcatttagccgtagtgatgttctggttcgtcatcagagaacccacacgg
gtggtggtagcggtggtggttcaggtggtagttactagatggctaactacttcaatacactgaatctgcgccagcagct
ggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagta
gtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctc
tgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacga
agaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacag
ccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaag
atatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtacc
gacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaacc
ggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcc
tgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcaga
aaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctc
tctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacata
tggacgacatcatctccggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctg
gcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgat
aaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattg
aagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaat
gaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgttt
atggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtga
acgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgc
tgttgcgggttaatactagagaaagaggagaaatactagagcccttccagtgtcgcatttgcatgcggaacttttcgct
caggacggaccttgacaggcatacccgtactcataccggtgaaaaaccgtttcagtgtcggatctgtatgcgaaatttc
tccctcagccagaccttgcgccgccatctacgtacgcacaccggcgagaagccattccaatgccgaatatgcatgcgca
acttcagtctcaggagcaacctggggcggcacctaaaaacccacacaggagaaaaatactagatgcctaagtaccgttc
cgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttc
ggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaac
tggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccat
gggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccac
tgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaata
ttccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatct
ggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccg
acctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgg
gcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgac
caaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatg
acgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgact
tcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaatacca
tatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgat
gtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaa
aaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctgga
cgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggt
aactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaag
tgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgcta
tgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaa
gcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcgg
caagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttaca
ggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgt
gaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaat
cgaaactggggggttaatactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggta
ctagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttta
acaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgc
tcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattt
tttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacgg
cctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcat
tatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaaga
aagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagag
ccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaa
acgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtc
agtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccac
gacgctatacccaaaagaaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgag
cactactagagtcacacaggaaagtactagatgtatacagtaggagattacctattagaccgattacacgagttaggaa
ttgaagaaatttttggagtccctggagactataacttacaatttttagatcaaattatttcccgcaaggatatgaaatg
ggtcggaaatgctaatgaattaaatgcttcatatatggctgatggctatgctcgtactaaaaaagctgccgcatttctt
acaacctttggagtaggtgaattgagtgcagttaatggattagcaggaagttacgccgaaaatttaccagtagtagaaa
tagtgggatcacctacatcaaaagttcaaaatgaaggaaaatttgttcatcatacgctggctgacggtgattttaaaca
ctttatgaaaatgcacgaacctgttacagcagctcgaactttactgacagcagaaaatgcaaccgttgaaattgaccga
gtactttctgcactattaaaagaaagaaaacctgtctatatcaacttaccagttgatgttgctgctgcaaaagcagaga
aaccctcactccctttgaaaaaagaaaactcaacttcaaatacaagtgaccaagagatcttgaacaaaattcaagaaag
cttgaaaaatgccaaaaaaccaatcgtgattacaggacatgaaataattagttttggcttagaaaaaacagtctctcaa
tttatttcaaagacaaaactacctattacgacattaaactttggaaaaagttcagttgatgaagctctcccttcatttt
taggaatctataatggtaaactctcagagcctaatcttaaagtattcgtggaatcagccgacttcatcctgatgcttgg
agttaaactcacagactcttcaacaggagccttcactcatcatttaaatgaaaataaaatgatttcactgaatatagat
gaaggaaaaatatttaacgaaagcatccaaaattttgattttgaatccctcatctcctctctcttagacctaagcgaaa
tagaatacaaaggaaaatatatcgataaaaagcaagaagactttgttccatcaaatgcgcttttatcacaagaccgcct
atggcaagcagttgaaaacctaactcaaagcaatgaaacaatcgttgctgaacaagggacatcattctttggcgcttca
tcaattttcttaaaaccaaagagtcattttattggtcaacccttatggggatcaattggatatacattcccagcagcat
taggaagccaaattgcagataaagaaagcagacaccttttatttattggtgatggttcacttcaacttacggtgcaaga
attaggattagcaatcagagaaaaaattaatccaatttgctttattatcaataatgatggttatacagtcgaaagagaa
attcatggaccaaatcaaagctacaatgatattccaatgtggaattactcaaaattaccagaatcatttggagcaacag
aagaacgagtagtctcgaaaatcgttagaactgaaaatgaatttgtgtctgtcatgaaagaagctcaagcagatccaaa
tagaatgtactggattgagttaattttggcaaaagaagatgcaccaaaagtactgaaaaaaatgggcaaactatttgct
gaacaaaataaatcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata