LOCUS       BBa_K887040             2244 bp    DNA     linear       26-Nov-2024
DEFINITION  Plac+B0034+zif268+alss
ACCESSION   BBa_K887040
VERSION     BBa_K887040.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    229..522
                     /label=BBa_K323105
     CDS             529..2244
                     /label=AlsS
     CDS             529..2244
                     /label=BBa_K539713
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagcc gggtgaaaaa
      241 ccgtatgcat gtccggttga aagctgtgat cgtcgtttta gccgtagtga tgaactgacc
      301 cgtcatattc gtattcatac aggtcagaaa ccgtttcagt gtcgtatttg catgcgtaat
      361 tttagccgtt cagatcatct gaccacccat attcgtaccc atactggcga aaaaccgttt
      421 gcctgtgata tttgtggtcg taaatttgca cgttccgatg aacgtaaacg ccataccaaa
      481 attcatacgg gtggtggtag cggtggtggt tcaggtggta gttactagat gttgacaaaa
      541 gcaacaaaag aacaaaaatc ccttgtgaaa aacagagggg cggagcttgt tgttgattgc
      601 ttagtggagc aaggtgtcac acatgtattt ggcattccag gtgcaaaaat tgatgcggta
      661 tttgacgctt tacaagataa aggacctgaa attatcgttg cccggcacga acaaaacgca
      721 gcattcatgg cccaagcagt cggccgttta actggaaaac cgggagtcgt gttagtcaca
      781 tcaggaccgg gtgcctctaa cttggcaaca ggcctgctga cagcgaacac tgaaggagac
      841 cctgtcgttg cgcttgctgg aaacgtgatc cgtgcagatc gtttaaaacg gacacatcaa
      901 tctttggata atgcggcgct attccagccg attacaaaat acagtgtaga agttcaagat
      961 gtaaaaaata taccggaagc tgttacaaat gcatttagga tagcgtcagc agggcaggct
     1021 ggggccgctt ttgtgagctt tccgcaagat gttgtgaatg aagtcacaaa tacgaaaaac
     1081 gtgcgtgctg ttgcagcgcc aaaactcggt ccagcagcag atgatgcaat cagtgcggcc
     1141 atagcaaaaa tccaaacagc aaaacttcct gtcgttttgg tcggcatgaa aggcggaaga
     1201 ccggaagcaa ttaaggcggt tcgcaagctt ttgaaaaagg ttcagcttcc atttgttgaa
     1261 acatatcaag ctgccggtac cctttccaga gatttagagg atcaatattt tggccgtatc
     1321 ggtttgttcc gcaaccagcc tggcgattta ctgctagagc aggcagatgt tgttctgacg
     1381 atcggctatg acccgattga atatgatccg aaattctgga atatcaatgg agaccggaca
     1441 attatccatt tagacgagat tatcgctgac attgatcatg cttaccagcc tgatcttgaa
     1501 ttgatcggtg acattccgtc cacgatcaat catatcgaac acgatgctgt gaaagtggaa
     1561 tttgcagagc gtgagcagaa aatcctttct gatttaaaac aatatatgca tgaaggtgag
     1621 caggtgccag cagattggaa atcagacaga gcgcaccctc ttgaaatcgt taaagagttg
     1681 cgtaatgcag tcgatgatca tgttacagta acttgcgata tcggttcgca cgccatttgg
     1741 atgtcacgtt atttccgcag ctacgagccg ttaacattaa tgatcagtaa cggtatgcaa
     1801 acactcggcg ttgcgcttcc ttgggcaatc ggcgcttcat tggtgaaacc gggagaaaaa
     1861 gtggtttctg tctctggtga cggcggtttc ttattctcag caatggaatt agagacagca
     1921 gttcgactaa aagcaccaat tgtacacatt gtatggaacg acagcacata tgacatggtt
     1981 gcattccagc aattgaaaaa atataaccgt acatctgcgg tcgatttcgg aaatatcgat
     2041 atcgtgaaat atgcggaaag cttcggagca actggcttgc gcgtagaatc accagaccag
     2101 ctggcagatg ttctgcgtca aggcatgaac gctgaaggtc ctgtcatcat cgatgtcccg
     2161 gttgactaca gtgataacat taatttagca agtgacaagc ttccgaaaga atttggggaa
     2221 ctcatgaaaa cgaaagctct ctag
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