LOCUS       BBa_K887051             2398 bp    DNA     linear       13-May-2024
DEFINITION  Ptet(promoter)+B0032(RBS)+Gli1(zinc finger)+kivD(enzyme)+B0015(term
            inator)
ACCESSION   BBa_K887051
VERSION     BBa_K887051.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    84..608
                     /label=BBa_K165007
     CDS             615..2261
                     /label=kivd coding sequence
     CDS             615..2261
                     /label=BBa_K539714
     stem_loop       2281..2324
                     /label=stem_loop
     misc_feature    2270..2349
                     /label=BBa_B0010
     terminator      2270..2349
                     /label=BBa_B0010
     stem_loop       2365..2384
                     /label=T7 TE
     misc_feature    2391..2391
                     /label=stop
     polyA_site      2385..2398
                     /label=polya
     misc_feature    2358..2398
                     /label=BBa_B0012
     terminator      2358..2398
                     /label=BBa_B0012
     terminator      2270..2398
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gagaagcgtg agcctgaatc tgtgtatgaa actgactgcc
      121 gttgggatgg ctgtagccag gaatttgact cccaagagca gctggtgcac cacatcaaca
      181 gcgagcacat ccacggggag cggaaggagt tcgtgtgcca ctgggggggc tgctccaggg
      241 agctgaggcc cttcaaagcc cagtacatgc tggtggttca catgcgcaga cacactggcg
      301 agaagccaca caagtgcacg tttgaagggt gccggaagtc atactcacgc ctcgaaaacc
      361 tgaagacgca cctgcggtca cacacgggtg agaagccata catgtgtgag cacgagggct
      421 gtagtaaagc cttcagcaat gccagtgacc gagccaagca ccagaatcgg acccattcca
      481 atgagaagcc gtatgtatgt aagctccctg gctgcaccaa acgctataca gatcctagct
      541 cgctgcgaaa acatgtcaag acagtgcatg gtcctgacgc ccatgtgacc aaacggcacc
      601 gtggggatta ctagatgtat acagtaggag attacctatt agaccgatta cacgagttag
      661 gaattgaaga aatttttgga gtccctggag actataactt acaattttta gatcaaatta
      721 tttcccgcaa ggatatgaaa tgggtcggaa atgctaatga attaaatgct tcatatatgg
      781 ctgatggcta tgctcgtact aaaaaagctg ccgcatttct tacaaccttt ggagtaggtg
      841 aattgagtgc agttaatgga ttagcaggaa gttacgccga aaatttacca gtagtagaaa
      901 tagtgggatc acctacatca aaagttcaaa atgaaggaaa atttgttcat catacgctgg
      961 ctgacggtga ttttaaacac tttatgaaaa tgcacgaacc tgttacagca gctcgaactt
     1021 tactgacagc agaaaatgca accgttgaaa ttgaccgagt actttctgca ctattaaaag
     1081 aaagaaaacc tgtctatatc aacttaccag ttgatgttgc tgctgcaaaa gcagagaaac
     1141 cctcactccc tttgaaaaaa gaaaactcaa cttcaaatac aagtgaccaa gagatcttga
     1201 acaaaattca agaaagcttg aaaaatgcca aaaaaccaat cgtgattaca ggacatgaaa
     1261 taattagttt tggcttagaa aaaacagtct ctcaatttat ttcaaagaca aaactaccta
     1321 ttacgacatt aaactttgga aaaagttcag ttgatgaagc tctcccttca tttttaggaa
     1381 tctataatgg taaactctca gagcctaatc ttaaagtatt cgtggaatca gccgacttca
     1441 tcctgatgct tggagttaaa ctcacagact cttcaacagg agccttcact catcatttaa
     1501 atgaaaataa aatgatttca ctgaatatag atgaaggaaa aatatttaac gaaagcatcc
     1561 aaaattttga ttttgaatcc ctcatctcct ctctcttaga cctaagcgaa atagaataca
     1621 aaggaaaata tatcgataaa aagcaagaag actttgttcc atcaaatgcg cttttatcac
     1681 aagaccgcct atggcaagca gttgaaaacc taactcaaag caatgaaaca atcgttgctg
     1741 aacaagggac atcattcttt ggcgcttcat caattttctt aaaaccaaag agtcatttta
     1801 ttggtcaacc cttatgggga tcaattggat atacattccc agcagcatta ggaagccaaa
     1861 ttgcagataa agaaagcaga caccttttat ttattggtga tggttcactt caacttacgg
     1921 tgcaagaatt aggattagca atcagagaaa aaattaatcc aatttgcttt attatcaata
     1981 atgatggtta tacagtcgaa agagaaattc atggaccaaa tcaaagctac aatgatattc
     2041 caatgtggaa ttactcaaaa ttaccagaat catttggagc aacagaagaa cgagtagtct
     2101 cgaaaatcgt tagaactgaa aatgaatttg tgtctgtcat gaaagaagct caagcagatc
     2161 caaatagaat gtactggatt gagttaattt tggcaaaaga agatgcacca aaagtactga
     2221 aaaaaatggg caaactattt gctgaacaaa ataaatcata atactagagc caggcatcaa
     2281 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2341 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//