LOCUS BBa_K887070 3600 bp DNA linear 22-Nov-2024 DEFINITION Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC ACCESSION BBa_K887070 VERSION BBa_K887070.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 misc_feature 93..96 /label=conserved RBS 89..100 /label=BBa_B0034 CDS 107..1822 /label=AlsS CDS 107..1822 /label=BBa_K539713 misc_feature 1831..2118 /label=BBa_K323107 CDS 2125..3600 /label=ilvC coding sequence CDS 2125..3600 /label=BBa_K539711 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagaa agaggagaaa tactagatgt tgacaaaagc 121 aacaaaagaa caaaaatccc ttgtgaaaaa cagaggggcg gagcttgttg ttgattgctt 181 agtggagcaa ggtgtcacac atgtatttgg cattccaggt gcaaaaattg atgcggtatt 241 tgacgcttta caagataaag gacctgaaat tatcgttgcc cggcacgaac aaaacgcagc 301 attcatggcc caagcagtcg gccgtttaac tggaaaaccg ggagtcgtgt tagtcacatc 361 aggaccgggt gcctctaact tggcaacagg cctgctgaca gcgaacactg aaggagaccc 421 tgtcgttgcg cttgctggaa acgtgatccg tgcagatcgt ttaaaacgga cacatcaatc 481 tttggataat gcggcgctat tccagccgat tacaaaatac agtgtagaag ttcaagatgt 541 aaaaaatata ccggaagctg ttacaaatgc atttaggata gcgtcagcag ggcaggctgg 601 ggccgctttt gtgagctttc cgcaagatgt tgtgaatgaa gtcacaaata cgaaaaacgt 661 gcgtgctgtt gcagcgccaa aactcggtcc agcagcagat gatgcaatca gtgcggccat 721 agcaaaaatc caaacagcaa aacttcctgt cgttttggtc ggcatgaaag gcggaagacc 781 ggaagcaatt aaggcggttc gcaagctttt gaaaaaggtt cagcttccat ttgttgaaac 841 atatcaagct gccggtaccc tttccagaga tttagaggat caatattttg gccgtatcgg 901 tttgttccgc aaccagcctg gcgatttact gctagagcag gcagatgttg ttctgacgat 961 cggctatgac ccgattgaat atgatccgaa attctggaat atcaatggag accggacaat 1021 tatccattta gacgagatta tcgctgacat tgatcatgct taccagcctg atcttgaatt 1081 gatcggtgac attccgtcca cgatcaatca tatcgaacac gatgctgtga aagtggaatt 1141 tgcagagcgt gagcagaaaa tcctttctga tttaaaacaa tatatgcatg aaggtgagca 1201 ggtgccagca gattggaaat cagacagagc gcaccctctt gaaatcgtta aagagttgcg 1261 taatgcagtc gatgatcatg ttacagtaac ttgcgatatc ggttcgcacg ccatttggat 1321 gtcacgttat ttccgcagct acgagccgtt aacattaatg atcagtaacg gtatgcaaac 1381 actcggcgtt gcgcttcctt gggcaatcgg cgcttcattg gtgaaaccgg gagaaaaagt 1441 ggtttctgtc tctggtgacg gcggtttctt attctcagca atggaattag agacagcagt 1501 tcgactaaaa gcaccaattg tacacattgt atggaacgac agcacatatg acatggttgc 1561 attccagcaa ttgaaaaaat ataaccgtac atctgcggtc gatttcggaa atatcgatat 1621 cgtgaaatat gcggaaagct tcggagcaac tggcttgcgc gtagaatcac cagaccagct 1681 ggcagatgtt ctgcgtcaag gcatgaacgc tgaaggtcct gtcatcatcg atgtcccggt 1741 tgactacagt gataacatta atttagcaag tgacaagctt ccgaaagaat ttggggaact 1801 catgaaaacg aaagctctct agtactagag ccgggtgaaa aaccgtatgc atgtcctgaa 1861 tgtggtaaaa gctttagcca gcgtgcaaat ctgcgtgcac atcagcgtac ccatacaggt 1921 gaaaagcctt ataaatgccc agaatgcggc aaaagctttt cacgtagcga tcatctgacc 1981 acccatcagc gcacacatac tggcgagaaa ccttacaaat gtccagagtg tggtaaatca 2041 tttagccgta gtgatgttct ggttcgtcat cagagaaccc acacgggtgg tggtagcggt 2101 ggtggttcag gtggtagtta ctagatggct aactacttca atacactgaa tctgcgccag 2161 cagctggcac agctgggcaa atgtcgcttt atgggccgcg atgaatttgc cgatggcgcg 2221 agctaccttc agggtaaaaa agtagtcatc gtcggctgtg gcgcacaggg tctgaaccag 2281 ggcctgaaca tgcgtgattc tggtctcgat atctcctacg ctctgcgtaa agaagcgatt 2341 gccgagaagc gcgcgtcctg gcgtaaagcg accgaaaatg gttttaaagt gggtacttac 2401 gaagaactga tcccacaggc ggatctggtg attaacctga cgccggacaa gcagcactct 2461 gatgtagtgc gcaccgtaca gccactgatg aaagacggcg cggcgctggg ctactcgcac 2521 ggtttcaaca tcgtcgaagt gggcgagcag atccgtaaag atatcaccgt agtgatggtt 2581 gcgccgaaat gcccaggcac cgaagtgcgt gaagagtaca aacgtgggtt cggcgtaccg 2641 acgctgattg ccgttcaccc ggaaaacgat ccgaaaggcg aaggcatggc gattgccaaa 2701 gcctgggcgg ctgcaaccgg tggtcaccgt gcgggtgtgc tggaatcgtc cttcgttgcg 2761 gaagtgaaat ctgatctgat gggcgagcaa accatcctgt gcggtatgtt gcaggctggc 2821 tctctgctgt gcttcgacaa gctggtggaa gaaggtaccg atccagcata cgcagaaaaa 2881 ctgattcagt tcggttggga aaccatcacc gaagcactga aacagggcgg catcaccctg 2941 atgatggacc gtctctctaa cccggcgaaa ctgcgtgctt atgcgctttc tgaacagctg 3001 aaagagatca tggcacccct gttccagaaa catatggacg acatcatctc cggcgaattt 3061 tcttccggta tgatggcgga ctgggccaac gatgataaga aactgctgac ctggcgtgaa 3121 gagaccggca aaaccgcgtt tgaaaccgcg ccgcagtatg aaggcaaaat cggcgagcag 3181 gagtacttcg ataaaggcgt actgatgatt gcgatggtga aagcgggcgt tgaactggcg 3241 ttcgaaacca tggtcgattc cggcatcatt gaagagtctg catattatga atcactgcac 3301 gagctgccgc tgattgccaa caccatcgcc cgtaagcgtc tgtacgaaat gaacgtggtt 3361 atctctgata ccgctgagta cggtaactat ctgttctctt acgcttgtgt gccgttgctg 3421 aaaccgttta tggcagagct gcaaccgggc gacctgggta aagctattcc ggaaggcgcg 3481 gtagataacg ggcaactgcg tgatgtgaac gaagcgattc gcagccatgc gattgagcag 3541 gtaggtaaga aactgcgcgg ctatatgaca gatatgaaac gtattgctgt tgcgggttaa //